scholarly journals Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model

Critical Care ◽  
2010 ◽  
Vol 14 (4) ◽  
pp. R129 ◽  
Author(s):  
Matthias Lange ◽  
Rhykka Connelly ◽  
Daniel L Traber ◽  
Atsumori Hamahata ◽  
Yoshimitsu Nakano ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Time Course ◽  
Nitrosative Stress ◽  
Nitric Oxide Synthases ◽  
Adp Ribosylation ◽  
Sepsis Model

Time course and cellular localization of inducible nitric oxide synthases expression during cardiac allograft rejection

1999 ◽  
Vol 67 (3) ◽  
pp. 716-722 ◽  
Author(s):  
Neil K Worrall ◽  
Thomas P Misko ◽  
Mitchell D Botney ◽  
Patrick M Sullivan ◽  
Jia-J Hui ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Allograft Rejection ◽  
Cellular Localization ◽  
Time Course ◽  
Nitric Oxide Synthases ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection ◽  
Inducible Nitric Oxide

In vivo and in vitro approaches demonstrate proline is not directly involved in the protection against superoxide, nitric oxide, nitrogen dioxide and peroxynitrite

2016 ◽  
Vol 43 (9) ◽  
pp. 870 ◽  
Author(s):  
Santiago Signorelli ◽  
Camila Imparatta ◽  
Marta Rodríguez-Ruiz ◽  
Omar Borsani ◽  
Francisco J. Corpas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitrogen Dioxide ◽  
Time Course ◽  
Nitrosative Stress ◽  
Lotus Japonicus ◽  
Protein Nitration ◽  
Course Content

Plants accumulate proline under diverse types of stresses, and it has been suggested that this α-amino acid has the capacity to protect against oxidative stress. However, it is still controversial whether its protection is due to the direct scavenging of reactive oxygen species (ROS). To solve this issue and considering that nitrosative stress is directly related with an oxidative stress condition, we evaluated whether proline can protect against nitrosative damage. Using proteins of Lotus japonicus (Regel) K.Larsen leaves exposed to a peroxynitrite (ONOO–/ONOOH) generator in presence and absence of 100mM proline, the potential of proline to protect was analysed by the protein nitration profile and NADP-dependent isocitrate dehydrogenase activity, which is inhibited by nitration. In both cases, the presence of proline did not diminish the peroxynitrite effects. Additionally, proline biosynthesis Arabidopsis knockout (KO) mutant plants of Δ(1)-pyrroline-5-carboxylate synthetase1 (P5CS1) gene, designated as Atp5cs1-1 and Atp5cs1-4, showed similar protein nitration levels as wild-type plants under salinity-induced oxidative stress, despite mutants having higher levels of lipid oxidation, H2O2 and superoxide (O2·–). Finally, by a fluorometric assay using specific fluorescent probes, it was determined that the presence of 100mM proline did not affect the time-course content of peroxynitrite or nitric oxide generation in vitro. Our results reveal the relevance of proline accumulation in vivo under stress, but unequivocally demonstrate that proline is not a direct scavenger of peroxynitrite, superoxide, ·NO and nitrogen dioxide (·NO2).

scholarly journals Cerebral Microvascular Damage Occurs Early after Hypoxia–Ischemia via nNOS Activation in the Neonatal Brain

2014 ◽  
Vol 34 (4) ◽  
pp. 668-676 ◽  
Author(s):  
Yi-Ching Hsu ◽  
Ying-Chao Chang ◽  
Yung-Chieh Lin ◽  
Chun-I Sze ◽  
Chao-Ching Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Brain Damage ◽  
Cerebral Perfusion ◽  
Nitrosative Stress ◽  
Neuronal Damage ◽  
Cell Damage ◽  
Nitric Oxide Synthases ◽  
Neonatal Brain ◽  
Microvascular Damage ◽  
Vascular Lumen

Microvascular injury early after hypoxic ischemia (HI) may contribute to neonatal brain damage. N-methyl-D-aspartate receptor overstimulation activates neuronal nitric oxide synthases (nNOS). We hypothesized that microvascular damage occurs early post-HI via nNOS activation and contributes to brain injury. Postpartum day-7 rat pups were treated with 7-nitroindazole (7-NI) or aminoguanidine (AG) before or after HI. Electron microscopy was performed to measure neuronal and endothelial cell damage. There were vascular lumen narrowing at 1 hour, pyknotic neurons at 3 hours, and extensive neuronal damage and loss of vessels at 24 hours post HI. Early after reoxygenation, there were neurons with heterochromatic chromatin and endothelial cells with enlarged nuclei occluding the lumen. There was also increased 3-nitrotyrosin in the microvessels and decreased cerebral blood perfusion. 7-NI and AG treatment before hypoxia provided complete and partial neuroprotection, respectively. Early post-reoxygenation, the AG group showed significantly increased microvascular nitrosative stress, microvascular interruptions, swollen nuclei that narrowed the vascular lumen, and decreased cerebral perfusion. The 7-NI group showed significantly decreased microvascular nitrosative stress, patent vascular lumen, and increased cerebral perfusion. Our results indicate that microvascular damage occurs early and progressively post HI. Neuronal nitric oxide synthases activation contributes to microvascular damage and decreased cerebral perfusion early after reoxygenation and worsens brain damage.

Expression of Nitric Oxide Synthases (NOS) in rat uterus and cervix after LPS administration to pregnant and nonpregnant rats

1998 ◽  
Vol 5 (1) ◽  
pp. 145A-145A
Author(s):  
M ALI ◽  
I BUHIMSCHI ◽  
P ORISE ◽  
V JAIN ◽  
G SAADE ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthases ◽  
Rat Uterus
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