Divergent evolution of rice blast resistance Pi54 locus in the genus Oryza

Abstract MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of rice blast disease. Osa-miR162a targets Dicer-like 1 (DCL1) genes, which play vital roles in miRNA biogenesis and act as negative regulators in rice immunity. Here we demonstrate that Osa-miR162a improves rice immunity against M. oryzae and balances the trade-off between rice yield and resistance. Overexpression of Osa-miR162a compromises rice susceptibility to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H2O2). In contrast, blocking miR162 by overexpressing a target mimic of miR162 enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H2O2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in reduced yield per plant, whereas blocking miR162 leads to an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of miR162 had limited impact on the expression of OsDCL1. Together, our results indicate that Osa-miR162a improves rice blast resistance and plays a role in the balance of trade-off between resistance and yield.

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Screening of the Dominant Rice Blast Resistance Genes with PCR-based SNP and CAPS Marker in Aromatic Rice Germplasm

Korean Journal of Crop Science ◽

10.7740/kjcs.2011.56.4.329 ◽

2011 ◽

Vol 56 (4) ◽

pp. 329-341 ◽

Cited By ~ 1

Author(s):

Jeong-Soon Kim ◽

Sang-Nag Ahn ◽

Sung-Jun Hong ◽

Jin-Hyeuk Kwon ◽

Yeong-Ki Kim ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Aromatic Rice ◽

Caps Marker ◽

Rice Blast Resistance ◽

Rice Germplasm ◽

Blast Resistance Genes

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Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population

Cropp Breeding and Applied Biotechnology ◽

10.12702/1984-7033.v10n01a04 ◽

2010 ◽

Vol 10 (1) ◽

pp. 23-31 ◽

Cited By ~ 2

Author(s):

L.G. Araújo ◽

A.S. Prabhu ◽

P.A.A. Pereira ◽

G.B. Silva

Keyword(s):

Resistance Gene ◽

Marker Assisted Selection ◽

Rice Blast ◽

Blast Resistance ◽

Backcross Population ◽

Rice Blast Resistance ◽

Blast Resistance Gene ◽

Selection For

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Identification of rice blast (Pyricularia) resistance genes in the breeding samples of rice

E3S Web of Conferences ◽

10.1051/e3sconf/202017501008 ◽

2020 ◽

Vol 175 ◽

pp. 01008

Author(s):

Nataliya Vozhzhova ◽

Elena Krasnova ◽

Elena Ionova

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Agricultural Research ◽

Blast Resistance ◽

Genetic Material ◽

Climatic Conditions ◽

Rice Blast Resistance ◽

Rice Samples ◽

Functional Alleles ◽

Blast Resistance Genes

The current paper has considered the challenge of breeding of rice lines adapted to the climatic conditions of the Southern Federal District of Russia by pyramiding a complex of Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes which is efficient in the southern part of Russia. The control of the resistance genes present in the breeding material was carried out using a marker-associated selection (MAS). The purpose of the current study was to identify the Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b rice blast resistance genes in the rice samples developed in the laboratory of rice breeding and seed production of the FSBSI “Agricultural Research Center “Donskoy” to identify valuable genotypes. The screening of 689 breeding samples of rice resulted in identification of the allelic state of the Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes, and the genotypes with functional alleles of these genes and their combinations. There were identified 19 breeding samples of rice possessing a combination of 4 functional alleles of rice blast resistance genes in various combinations. The identified rice samples are valuable genetic material and are recommended to use as the sources of rice blast resistance in the breeding process.

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