Genetic diversity of Elaeis oleifera (HBK) Cortes populations using cross species SSRs: implication’s for germplasm utilization and conservation

The oil palm species of Elaeis oleifera have some superior characters not possessed by E. guineensis species. The cross between E. oleifera x E. guineensis (E. o x E. g.) Is carried out to insert the superior character of E. oleifera into the E. guineensis species and to obtain individuals who possess a superior characteristic blend of both species. This study aims to determine the genetic diversity within and between populations of E. oleifera, as well as relationships between E. oleifera, E. guineensis and its interspecific hybrids. A total of 8 germplasm populations used were E. oleifera originating from Suriname and Brazil, E. guineensis population of Dura Deli and SP540T, and interspecific crosses of hybrid E. guineensis x E. oleifera from Colombia, hybrid E. guineensis x E. oleifera which is presumably from Colombia, as well as hybrid E. guineensis x E. oleifera from Brazil. A total of sixteen Simple Sequence Repeat (SSR) markers were used to analyze 92 individuals from 8 populations. PCoA results show that 8 Elaeis populations cluster in each group. The hybrid population of E. guineensis x E. oleifera suspected of Colombia has a very close kinship relationship with hybrid E. guineensis x E. oleifera from Colombia and clumped in the same quadrant. This suggests that the hybrid is indeed a hybrid of E. guineensis x E. oleifera from Colombia. The population of E. oleifera from Suriname showed the lowest genetic diversity, with the number of different alleles, specific alleles, heterozygosity values, and the percentages of polymorphic loci sequentially were 1.37, 0.18, 0.09, and 37.50%. While the population of interspecific interspecific crossbreeds of the E. oleifera species showed the highest genetic diversity with values of 3.81, 0.43, 0.62 and 100%.

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Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2002000800008 ◽

2002 ◽

Vol 37 (8) ◽

pp. 1105-1114 ◽

Cited By ~ 31

Author(s):

Edson Barcelos ◽

Philippe Amblard ◽

Julien Berthaud ◽

Marc Seguin

Keyword(s):

Genetic Diversity ◽

Length Polymorphism ◽

Fragment Length ◽

Oil Palm ◽

Fragment Length Polymorphism ◽

Genetic Relationships ◽

Aflp Analysis ◽

Elaeis Oleifera ◽

French Guyana ◽

And Cluster Analysis

The objective of this work was to evaluate the genetic diversity, its organization and the genetic relationships within oil palm (Elaeis oleifera (Kunth) Cortés, from America, and E. guineensis (Jacq.), from Africa) germplasm using Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP). In complement to a previous RFLP study on 241 E. oleifera accessions, 38 E. guineensis accessions were analyzed using the same 37 cDNA probes. These accessions covered a large part of the geographical distribution areas of these species in America and Africa. In addition, AFLP analysis was performed on a sub-set of 40 accessions of E. oleifera and 22 of E. guineensis using three pairs of enzyme/primer combinations. Data were subjected to Factorial Analysis of Correspondence (FAC) and cluster analysis, with parameters of genetic diversity being also studied. Results appeared congruent between RFLP and AFLP. In the E. oleifera, AFLP confirmed the strong structure of genetic diversity revealed by RFLP, according to geographical origin of the studied material, with the identification of the same four distinct genetic groups: Brazil, French Guyana/Surinam, Peru, north of Colombia/Central America. Both markers revealed that genetic divergence between the two species is of the same magnitude as that among provenances of E. oleifera. This finding is in discrepancy with the supposed early tertiary separation of the two species.

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GENETIC VARIATION OF E. oleifera POPULATION AND POPULATION E. guineensis x E. oleifera ON NUTFAH PLASMA COLLECTION PPKS

Jurnal Penelitian Kelapa Sawit ◽

10.22302/10.22302/iopri.jur.jpks.v24i1.3 ◽

2018 ◽

Vol 24 (1) ◽

pp. 13-22

Author(s):

Rokhana Faizah ◽

Sri Wening ◽

Hernawan Yuli Rahmadi ◽

Abdul Razak Purba

Keyword(s):

Genetic Diversity ◽

Simple Sequence Repeat ◽

Interspecific Hybrids ◽

Hybrid Population ◽

Interspecific Crosses ◽

Sequence Repeat ◽

Elaeis Oleifera ◽

Palm Species ◽

Plasma Collection ◽

Simple Sequence

The oil palm species of Elaeis oleifera have some superior characters not possessed by E. guineensis species. The cross between E. oleifera x E. guineensis (E. o x E. g.) Is carried out to insert the superior character of E. oleifera into the E. guineensis species and to obtain individuals who possess a superior characteristic blend of both species. This study aims to determine the genetic diversity within and between populations of E. oleifera, as well as relationships between E. oleifera, E. guineensis and its interspecific hybrids. A total of 8 germplasm populations used were E. oleifera originating from Suriname and Brazil, E. guineensis population of Dura Deli and SP540T, and interspecific crosses of hybrid E. guineensis x E. oleifera from Colombia, hybrid E. guineensis x E. oleifera which is presumably from Colombia, as well as hybrid E. guineensis x E. oleifera from Brazil. A total of sixteen Simple Sequence Repeat (SSR) markers were used to analyze 92 individuals from 8 populations. PCoA results show that 8 Elaeis populations cluster in each group. The hybrid population of E. guineensis x E. oleifera suspected of Colombia has a very close kinship relationship with hybrid E. guineensis x E. oleifera from Colombia and clumped in the same quadrant. This suggests that the hybrid is indeed a hybrid of E. guineensis x E. oleifera from Colombia. The population of E. oleifera from Suriname showed the lowest genetic diversity, with the number of different alleles, specific alleles, heterozygosity values, and the percentages of polymorphic loci sequentially were 1.37, 0.18, 0.09, and 37.50%. While the population of interspecific interspecific crossbreeds of the E. oleifera species showed the highest genetic diversity with values of 3.81, 0.43, 0.62 and 100%.

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