Diversity and abundance of pollinators in different winter crops at Sher-e-Bangla Agricultural University Campus, Dhaka

The study was conducted at Sher-e-Bangla Agricultural University campus to document the diversity and abundance of pollinators of five commonly grown winter crops, viz., mustard, coriander, niger, black cumin and linseed from October 2020 to February 2021. A total number of fifteen species was identified under eleven genera, nine subfamilies, nine families and seven super families. The study revealed that honey bees, halictids, nymphalids, coccinellids, butterflies and dipterans of genera Apis, Halictus, Lasioglossum, Aglais, Coccinella, Pieris, Eurema, Musca, Syrphid and Calliphora belonging to the families Apidae, Halictidae, Nymphalidae, Coccinellidae, Pieridae, Muscidae, Syrphidae and Calliphoridae, respectively were present in the field. The species diversity was high in mustard with 15 species while it was low in linseed (5 species). The honey bees, Apis sp. and sweat bees, Halictus sp. were common pollinators of all five oilseed crops, while the housefly, Musca domestica was specific to mustard. The honey bee, Apis sp. was predominant among all the insect pollinators in five oilseed crops. The Species Richness (SR), Shannon-Weaver index (H’), Community dominance and Question of similarity indices were applied to determine the diversity and abundance of pollinators. J. Biodivers. Conserv. Bioresour. Manag. 2021, 7(1): 33-42

Productivity of camelina and spring rapeseed as affected by varietal characteristics in the Right-Bank Forest-Steppe of Ukraine

Purpose. Determine the oil content and glucosinolate content and productivity of spring camelina and spring rapeseed as affected by varietal characteristics. Methods. The research was conducted in the educational-scientific-industrial complex of Uman National University of Horticulture. Spring rapeseed varieties ‘Belinda’, ‘Aidar’, ‘Heros’, ‘Jerry’ and spring camelina varieties ‘Zevs’, ‘Hirskyi’, ‘Slavutych’, ‘Mirazh’ were studied. Seeds were sown in a row to a depth of 1.5 cm with a seeder CH-16. The sowing rate was 300 seeds/m2, or 5.4 kg/ha. The crop was harvested separately by Sampo-500 combine. Results. In spring rapeseed, the highest yield was marked by variety ‘Aidar’ (2.52 t/ha) and the lowest by ‘Heros’ (2.43 t/ha). In spring camelina, the highest yield was in variety ‘Zevs’ (2.31 t/ha) and lower in ‘Hirska’ (2.15), ‘Mirazh’ (2.27), and ‘Slavutych’ (2.22 t/ha). The highest oil content among rapeseed varieties demonstrated ‘Aidar’ (43.7%). Slightly lower it was in ‘Belinda’ (43.2%), ‘Jerry’ (42.8) and ‘Heros’ (42.6%). Depending on the varietal characteristics, the highest percentage of oil content was found in spring camelina varieties ‘Zevs’ (45.1%). It was lower by 0.5 and 0.9% in ‘Mirazh’ and ‘Slavutych’. The lowest percentage of oil content (43.7%) was determined in camelina variety ‘Hirska. In spring rapeseed varieties, the highest content of glucosinolates was in ‘Aidar’ – 20.5 μmol/g, 0.3 and 0.5 μmol/g less in ‘Belinda’ and ‘Jerry’, and the lowest in ‘Heros’ (19.8 μmol/g). In spring camelina, the highest value was in the variety ‘Zevs’ (22.0 μmol/g), and the lowest in ‘Hirska’ (21.0 μmol/g). ‘Mirazh’ and ‘Slavutych’ contained 21.7 and 21.4 μmol/g of glucosinolatesm respectively. Conclusions. The productivity of oilseed crops varies under the same growing conditions: spring rapeseed, regardless of varietal characteristics, demonstrated higher yield compared to camelina. On average over the years of research and varieties, this indicator was 2.47 t in spring rapeseed, and 2.24 t/ha in spring camelina, which is 0.23 t/ha less. In rapeseed, the oil content of the seeds was 43.07%, the content of glucosinolates varied from 19.8 to 20.5 μmol/g. In spring camelina, the oil content of seeds was 1.33–44.40% higher, the content of glucosinolates ranged from 21.0 to 22.0 μmol/g. The level of profitability of the studied oilseed crops was high and varied as affected by varietal characteristics: in rapeseed from 122 to 141%, and in spring camelina from 182 to 196%.

Nematode RALF-Like 1 Targets Soybean Malectin-Like Receptor Kinase to Facilitate Parasitism

Soybean [Glycine max (L.) Merr. ] is one of the most strategical oilseed crops that provides sustainable source of protein and oil worldwide. Cultivation of soybean is severely affected by root-knot nematode (RKN). However, the mechanism of RKN parasitism to soybeans is largely unknown. In this study, we identify GmLMM1, which encodes a homolog of FERONIA-like receptor kinase in soybean, as a susceptible gene toward nematode. Mutations of GmLMM1 exhibit enhanced resistance against the RKN Meloidogyne incognita. RNA-sequencing (RNA-seq) analysis reveals a similar differential expression pattern for genes regulated by GmLMM1 (Gmlmm1 vs. wild-type) and M. incognita (M. incognita vs. mock), supporting the role of GmLMM1 in M. incognita infection. Unlike FERONIA in Arabidopsis, GmLMM1 specifically binds to MiRALF1 and AtRALF23 that suppress plant immunity, but not MiRALF3 and AtRALF1. Moreover, we found that the single-nucleotide polymorphism (SNP) in GmLMM1 leads to the natural resistance against RKNs in soybeans. Collectively, these findings uncover GmLMM1 as a susceptible target of nematode RALF-like 1 and provide new genetic resource for nematode resistant breeding.

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

Temperature is one of the decisive environmental factors that is projected to increase by 1. 5°C over the next two decades due to climate change that may affect various agronomic characteristics, such as biomass production, phenology and physiology, and yield-contributing traits in oilseed crops. Oilseed crops such as soybean, sunflower, canola, peanut, cottonseed, coconut, palm oil, sesame, safflower, olive etc., are widely grown. Specific importance is the vulnerability of oil synthesis in these crops against the rise in climatic temperature, threatening the stability of yield and quality. The natural defense system in these crops cannot withstand the harmful impacts of heat stress, thus causing a considerable loss in seed and oil yield. Therefore, a proper understanding of underlying mechanisms of genotype-environment interactions that could affect oil synthesis pathways is a prime requirement in developing stable cultivars. Heat stress tolerance is a complex quantitative trait controlled by many genes and is challenging to study and characterize. However, heat tolerance studies to date have pointed to several sophisticated mechanisms to deal with the stress of high temperatures, including hormonal signaling pathways for sensing heat stimuli and acquiring tolerance to heat stress, maintaining membrane integrity, production of heat shock proteins (HSPs), removal of reactive oxygen species (ROS), assembly of antioxidants, accumulation of compatible solutes, modified gene expression to enable changes, intelligent agricultural technologies, and several other agronomic techniques for thriving and surviving. Manipulation of multiple genes responsible for thermo-tolerance and exploring their high expressions greatly impacts their potential application using CRISPR/Cas genome editing and OMICS technology. This review highlights the latest outcomes on the response and tolerance to heat stress at the cellular, organelle, and whole plant levels describing numerous approaches applied to enhance thermos-tolerance in oilseed crops. We are attempting to critically analyze the scattered existing approaches to temperature tolerance used in oilseeds as a whole, work toward extending studies into the field, and provide researchers and related parties with useful information to streamline their breeding programs so that they can seek new avenues and develop guidelines that will greatly enhance ongoing efforts to establish heat stress tolerance in oilseeds.

Improvement of yield in Guizotia abyssinica (L.F.) Cass. by using backcross method

Guizotia abyssinica (L.F.) Cass. is one of the important oilseed crops cultivated in India. The seed of the plant is used by the tribal and rural people of our country as a source of edible oil. The present investigation of the Niger plant deals with the study of the back cross-program and improvement in the variety. Unknown local variety (A) of niger was selected as a non-recurrent parent because of having some desirable characters like less vegetative growth (dwarf), resistance to waterlogged condition, and large seed grains while IGP-76 variety (B) was selected as a recurrent parent and used as a female having characters like small black grains, resistant to leaf-eating cater Piller and powdery mildew result shows successfully transfer of the characters in the F1 generation. The average number of capitulum/plant shows very good and positive results in F1 as well as BC1 generation. As compared to the recurrent parent (B) an average number of capitulum per plant was increased in F1 generation by 14.28% and in BC1 generation by 28.5%. The table indicates that there is incensement in the average number of seeds per capitulum. The average number of seeds per capitulum was increased by 8.3% in the F1 generation and by 11.11 % in the BC1 generation as compared to the recurrent parent. Average weights of 1000 seeds were also increased in F1 generation by 31.78% and in BC1 generation by 47.28% along with the total yield per plant.

Probing the Metabolic Landscape of Plant Vascular Bundles by Infrared Fingerprint Analysis, Imaging and Mass Spectrometry

Fingerprint analysis is a common technique in forensic and criminal investigations. Similar techniques exist in the field of infrared spectroscopy to identify biomolecules according to their characteristic spectral fingerprint features. These unique markers are located in a wavenumber range from 1800 to 600 cm−1 in the mid infrared region. Here, a novel bioanalytical concept of correlating these spectral features with corresponding mass spectrometry datasets to unravel metabolic clusters within complex plant tissues was applied. As proof of concept, vascular bundles of oilseed rape (Brassica napus) were investigated, one of the most important and widely cultivated temperate zone oilseed crops. The link between mass spectrometry data and spectral data identified features that co-aligned within both datasets. Regions of origin were then detected by searching for these features in hyperspectral images of plant tissues. This approach, based on co-alignment and co-localization, finally enabled the detection of eight distinct metabolic clusters, reflecting functional and structural arrangements within the vascular bundle. The proposed analytical concept may assist future synergistic research approaches and may lead to biotechnological innovations with regard to crop yield and sustainability.

Virus Diseases of Cereal and Oilseed Crops in Australia: Current Position and Future Challenges

This review summarizes research on virus diseases of cereals and oilseeds in Australia since the 1950s. All viruses known to infect the diverse range of cereal and oilseed crops grown in the continent’s temperate, Mediterranean, subtropical and tropical cropping regions are included. Viruses that occur commonly and have potential to cause the greatest seed yield and quality losses are described in detail, focusing on their biology, epidemiology and management. These are: barley yellow dwarf virus, cereal yellow dwarf virus and wheat streak mosaic virus in wheat, barley, oats, triticale and rye; Johnsongrass mosaic virus in sorghum, maize, sweet corn and pearl millet; turnip yellows virus and turnip mosaic virus in canola and Indian mustard; tobacco streak virus in sunflower; and cotton bunchy top virus in cotton. The currently less important viruses covered number nine infecting nine cereal crops and 14 infecting eight oilseed crops (none recorded for rice or linseed). Brief background information on the scope of the Australian cereal and oilseed industries, virus epidemiology and management and yield loss quantification is provided. Major future threats to managing virus diseases effectively include damaging viruses and virus vector species spreading from elsewhere, the increasing spectrum of insecticide resistance in insect and mite vectors, resistance-breaking virus strains, changes in epidemiology, virus and vectors impacts arising from climate instability and extreme weather events, and insufficient industry awareness of virus diseases. The pressing need for more resources to focus on addressing these threats is emphasized and recommendations over future research priorities provided.