Multiple Anthropogenic Pressures Lead to Seed Dispersal Collapse of the Southernmost Palm Jubaea chilensis

Seed dispersal is a critical process for plant reproduction and regeneration. Successful recruitment depends on pre- and post-dispersal processes that complete a seed’s journey until becoming a new plant. However, anthropogenic stressors may disrupt the seed dispersal process at some stages, collapsing plant regeneration and hampering its long-term persistence. The Chilean palm tree Jubaea chilensis is the southernmost and the only non-tropical palm species, which currently relies on the scatter-hoarding rodent Octodon degus for seed dispersal. We assessed seed fate by measuring predation and dispersal rates through experimental fieldwork in the Palmar de Ocoa site (located within La Campana National Park) and the Palmar El Salto. We also used previous reports on seed harvest and seedling herbivory to depict the whole J. chilensis seed dispersal process and assess the relative importance of different anthropogenic pressures. We asked the following questions: (1) What is the effect of human harvesting on J. chilensis recruitment? (2) Do native and exotic rodents predate J. chilensis seeds in the same way? and (3) Does post-dispersal herbivory matter? We found that J. chilensis fruits are harvested for human consumption, reducing pre-dispersal available seeds by removing about 23 tons per season. Then, post-dispersal seeds at the Ocoa palm grove are heavily predated by exotic (Rattus rattus) and native (Octodon spp.) rodents; only 8.7% of the seeds are effectively dispersed by Octodon degus. At Palmar El Salto, 100% of the seeds were predated by Rattus rattus, precluding further analysis. Finally, 70% of the seedlings were consumed by exotic herbivores (mainly rabbits), resulting in a success rate of 1.81%. Only 7.9% of the surviving seedlings become infantile plants (4 year-old). Our assessment suggests that J. chilensis has aging populations with very few young individuals in disturbed sites to replace the old ones. For those reasons, we suggest increasing its conservation category to critically endangered as land-use change is rapidly fragmenting and shrinking the extant J. chilensis populations. We urge to take urgent actions to protect this relict palm, which otherwise may go extinct in the next decades.

Brazilian Amazonian palm-stem types and uses: a review

ABSTRACT Palms may be an important source of renewable raw material to replace wood, however, the uses of the stems of native species of the Brazil are known only at the local or regional level. We carried out a literature review on the traditional knowledge of the uses of the stems of palm species native to the Amazon biome in Brazil, and related the types of uses with morphological characteristics of the stems. The review resulted in information on 45 species with solitary or cespitose stems, and six stem-size types: tall (15 species), medium-short (3), medium (5), small (17), acaulescent (1) and climbing (4). We found 80 indications of stem use in seven categories and 14 subcategories. A similarity analysis showed that, in general, tall, medium-short, medium, small (≥ 10 cm in diameter) and climbing stem types, solitary or cespitous, are used for construction, furniture, handicrafts, utensils, tools and musical instruments. Only small stems (< 10 cm diameter) are used to manufacture weapons for hunting and fishing, and climbing stems are used in the manufacture of ropes. Stems of Socratea exorrhiza, Euterpe oleracea and Desmoncus polyacanthos are the most frequently used to meet subsistence needs in traditional communities in the Brazilian Amazon. Our findings indicate that there is a potential for use of several native palm stems as sources for alternative materials in the manufacture industry and as sustainable income sources for Amazonian communities.

Genetic Variability of Palm Lethal Decline Phytoplasmas in the Caribbean Basin and Florida, U.S.A., Based on a Multilocus Analysis

Palm lethal decline phytoplasmas are an important group of plant pathogens that cause death in a variety of palm species throughout the Caribbean basin and the southeastern United States. The 16SrIV-D phytoplasma was introduced to the state of Florida, United States; it has since caused severe economic losses to the green industries of Florida and is threating natural ecosystems because of its ability to infect the native palm Sabal palmetto. In this study, the genetic variability of the 16SrIV-D phytoplasma was assessed over a 10-year period to determine if multiple introductions had occurred or if natural mutations were occurring. Furthermore, the genetic variability of the palm lethal decline phytoplasma group (16SrIV) was assessed with a multiple locus analysis using the 16S ribosomal RNA gene, the 16S-23S intergenic spacer region, and secA and groEL genes. Overall, no variability of the 16SrIV-D phytoplasma was documented in Florida over a 10-year period. The multilocus analysis showed support for three distinct species of the phytoplasma in the Caribbean basin that infect palms and further support that the 16SrIV-C from Tanzania is not closely related. Furthermore, 16SrIV-B and 16SrIV-D were found to be the same phytoplasma based on 100% identity between the two based on intergenic spacer region, secA, and groEL analysis. This study represents the first robust, multilocus analysis of palm-infecting phytoplasmas from the Caribbean and sheds light on the phylogeny and evolution of the group.

Native palms as an economically important non-timber forest product among rural communities in the Yucatán peninsula, Mexico

Objective. To analyze the valuation, use, and preservation of native palms as a non-timber forest product (NTFP) of high economic importance for rural communities in the Yucatán Peninsula, Mexico. Design/methodology/approach: The available literature on palms and their use in the Yucatán Peninsula (YP) was examined using the snowball method. Subsequently, different online flora databases were consulted in order to examine the taxonomic identities of palm species present in the YP. The reported uses were classified. Results: The YP has 20 native species that belong to 13 genera and three subfamilies. All of them are economically exploited as NTFPs, especially in construction (85%) and honey production (70%), followed by food and medicinal use (35% each), craftwork (30%), ornamental use (25%), and fodder (10%). These data confirm that native palms are an important livelihood means for the inhabitants of Mayan communities in the region. Study limitations/implications: The research faced a limited database of encyclopedias, anthologies, directories, books, or articles that interpret works or research about this topic. Findings/Conclusions: The local and regional use of palms represents an additional income for the people who use this natural resource on different productive scales, both in rural communities and city centers. An alternative to ensure the sustainability of palms would be to establish governmental programs for their reproduction

An Efficient Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Protein 9 Mutagenesis System for Oil Palm (Elaeis guineensis)

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has emerged as a powerful tool for the precise editing of plant genomes for crop improvement. Rapid in vitro methods for the determination of guide RNA (gRNA) cleavage efficiency and an efficient DNA delivery system is essential for gene editing. However, we lack an efficient gene-editing system for palm species. In this study, we described the development of a transient oil palm protoplast assay to rapidly evaluate the cleavage efficiency of CRISPR/Cas9 mutagenesis and the generation of stable transformed oil palms using biolistic particle bombardment in immature embryos. Using the phytoene desaturase (EgPDS) gene, we found cleavage frequency of up to 25.49% in electro-transfected protoplast, which enables the production of transgenic oil palm shoots exhibiting chimeric albino phenotypes as a result of DNA insertions, deletions (InDels), and nucleotide substitutions, with a mutation efficiency of 62.5–83.33%. We further validated the mutagenesis efficiency and specificity of the CRISPR/Cas9 system in oil palm by targeting the brassinosteroid-insensitive 1 (EgBRI1) gene, which resulted in nucleotide substitutions in EgBRI1 with premature necrosis phenotype in oil palm transgenic shoots and stunted phenotype resulting from DNA InDels. Taken together, our results showed that effective and efficient editing of genes using the CRISPR/Cas9 system can be achieved in oil palm by optimizing the selection of efficient gRNA and DNA delivery methods. This newly designed strategy will enable new routes for the genetic improvement in oil palm and related species.

Linking Plant Functional Traits to Demography in a Fragmented Landscape

Habitat loss in highly deforested landscapes such as the Brazilian Atlantic Forest has been severely affecting the diversity and survival of palm species. As some species are more sensitive than others, trait responses to the environment, as well as environmental effects on fecundity, growth, and mortality rates, may affect species demography. Considering this context, we studied functional and demographic responses of three palm species (Astrocaryum aculeatissimum, Euterpe edulis, and Geonoma schottiana) to habitat loss in the Atlantic Forest in southeastern Brazil by measuring morpho-physiological traits related to plant growth and light acquisition for photosynthesis. We also tested the response of population fitness to fragment size. Plant survival and growth was subsequently monitored in 2006 and 2007, and population dynamics were summarized in pool matrices for large and small forest fragments in the monitoring periods comprehending one full year between 2005–2006 and 2006–2007. The asymptotic growth rate of populations (defined here as population fitness, λ) in five forest fragments was then calculated. Diameter of individuals of the demography plots (from year 2005 to 2007) was used to calculate the relative diameter growth rate. Later, in 2015, we measured a set of morpho-physiological functional traits in palms in the same plots used in the demographic studies. While A. aculeatissimum populations were stable in both monitoring periods in small and large fragments, E. edulis populations were predicted to decline due to intense predation by monkeys in the large fragment, but were stable in the smaller fragments, and G. schottiana populations were stable in the large fragments in both monitoring periods, but populations in the smaller fragments were predicted to decline in the second period, i.e., with lower fitness in these fragments. In addition, the functional traits analyzed showed that G. schottiana is a forest interior species associated with the shade/understory environment response. E. edulis was also affected by the size of the fragment, but due to a disruptive interaction with a predator and showed intermediate functional traits values. On the other hand, A. aculeatissimum thrived in areas with higher and lower incidence of light and was not demographically affected by forest remnant size. This suggests that E. edulis and A. aculeatissimum are habitat generalists. We concluded that differences in the ecophysiological performance of palms due to distinct morpho-physiological functional traits related to leaf economic spectrum, such as LDMC or specific leaf area (SLA) and to photosynthetic responses to light environment as electron transport rate (ETR) and saturation irradiance (Ik) were linked to the demographic variation observed in forest remnants of different size.

Environmental impact assessment of vernacular thatch building tradition in Mexico: Case studies of three palm species and related technology along the Trans-Mexican Volcanic Belt

<p>This project involves research into vernacular buildings, in particular traditional and contemporary coastal palm thatch buildings in the middle-west coast deciduous forest environments in Mexico. The fieldwork-based grounded theory research investigates the natural materials and techniques involved in constructing thatched vernacular buildings using three different palm species (Attalea guacuyule, Brahea dulcis and Sabal rosei) in different climatic contexts along the Trans Mexican Volcanic Belt (TMVB). The aim is to achieve a holistic approach to the environmental assessment of these building types through a life-cycle assessment. The present research emerges from the premise that vernacular buildings are generally taken for granted as sustainable, and yet they are under-investigated meaning this assumption may not be correct. In general the performance of vernacular buildings in terms of sustainability and in relation to their embodied energy is under reported, particularly in terms of vernacular case studies in Mexico. However the deterioration of the environments where such buildings are located through anthropogenic causes is of world-wide importance, therefore it is a priority for this research to study the relationship of the vernacular traditions with their immediate environment. The first section of the thesis analyses theoretical frameworks for sustainability and vernacular architecture. Both terms are widely used and carry many different meanings, so it is important to establish the definitions used in this research to better set the boundaries of the study as a basis for seeking the best methods for assessing the environmental impact of the selected vernacular thatch building technologies. The second section undertakes qualitative and quantitative fieldwork on traditional practices of palm thatch buildings in selected regions of Mexico and related case studies, six in total. The fieldwork was combined with investigation into ethnographical, ethnobotanical and historical records and data for the three palms used for thatching and their related materials and technologies in order to derive data concerning yield factors, lifespan of the building materials, carrying capacities, embodied energy of transport and embodied energy of materials. The third section applies the collected information for a life-cycle environmental assessment (LCA) of two typical buildings for each of the three different palm species and the diverse techniques involved. The assessment is carried out based on various assumptions that are commonly used in LCA to give a carbon account and an ecological footprint for each building component both after construction and for a 50 year building life. However, during the research particular variables in the analysis were revealed, such as operating energy, recycling of building elements, durability of materials and transport practices, which can vary widely from case to case, therefore the limits and scope of the assessment excluded such data for a better comparative scenario of the building process itself. This suggests that a different way of life-cycle accounting may be needed when assessing vernacular structures. The results show the environmental impact of these techniques in terms of carbon and ecological footprints, and reveal that the rural vernacular case studies had lower environmental impacts than the suburban and urban vernacular case studies as measured by their carbon content, energy expressed in Giga Joules (GJ) and their ecological footprints.</p>

Environmental impact assessment of vernacular thatch building tradition in Mexico: Case studies of three palm species and related technology along the Trans-Mexican Volcanic Belt

<p>This project involves research into vernacular buildings, in particular traditional and contemporary coastal palm thatch buildings in the middle-west coast deciduous forest environments in Mexico. The fieldwork-based grounded theory research investigates the natural materials and techniques involved in constructing thatched vernacular buildings using three different palm species (Attalea guacuyule, Brahea dulcis and Sabal rosei) in different climatic contexts along the Trans Mexican Volcanic Belt (TMVB). The aim is to achieve a holistic approach to the environmental assessment of these building types through a life-cycle assessment. The present research emerges from the premise that vernacular buildings are generally taken for granted as sustainable, and yet they are under-investigated meaning this assumption may not be correct. In general the performance of vernacular buildings in terms of sustainability and in relation to their embodied energy is under reported, particularly in terms of vernacular case studies in Mexico. However the deterioration of the environments where such buildings are located through anthropogenic causes is of world-wide importance, therefore it is a priority for this research to study the relationship of the vernacular traditions with their immediate environment. The first section of the thesis analyses theoretical frameworks for sustainability and vernacular architecture. Both terms are widely used and carry many different meanings, so it is important to establish the definitions used in this research to better set the boundaries of the study as a basis for seeking the best methods for assessing the environmental impact of the selected vernacular thatch building technologies. The second section undertakes qualitative and quantitative fieldwork on traditional practices of palm thatch buildings in selected regions of Mexico and related case studies, six in total. The fieldwork was combined with investigation into ethnographical, ethnobotanical and historical records and data for the three palms used for thatching and their related materials and technologies in order to derive data concerning yield factors, lifespan of the building materials, carrying capacities, embodied energy of transport and embodied energy of materials. The third section applies the collected information for a life-cycle environmental assessment (LCA) of two typical buildings for each of the three different palm species and the diverse techniques involved. The assessment is carried out based on various assumptions that are commonly used in LCA to give a carbon account and an ecological footprint for each building component both after construction and for a 50 year building life. However, during the research particular variables in the analysis were revealed, such as operating energy, recycling of building elements, durability of materials and transport practices, which can vary widely from case to case, therefore the limits and scope of the assessment excluded such data for a better comparative scenario of the building process itself. This suggests that a different way of life-cycle accounting may be needed when assessing vernacular structures. The results show the environmental impact of these techniques in terms of carbon and ecological footprints, and reveal that the rural vernacular case studies had lower environmental impacts than the suburban and urban vernacular case studies as measured by their carbon content, energy expressed in Giga Joules (GJ) and their ecological footprints.</p>

The Ecology of Palm Genomes: Repeat-associated genome size expansion is constrained by aridity

Genome size varies 2,400-fold across plants, influencing their evolution through changes in cell size and cell division rates which impact plants' environmental stress tolerance. Repetitive element expansion explains much genome size diversity, and the processes structuring repeat 'communities' are analogous to those structuring ecological communities. However, which environmental stressors influence repeat community dynamics has not yet been examined from an ecological perspective. We measured genome size and leveraged climatic data for 91% of genera within the ecologically diverse palm family (Arecaceae). We then generated genomic repeat profiles for 141 palm species, and analysed repeats using phylogenetically-informed linear models to explore relationships between repeat dynamics and environmental factors. We show that palm genome size and repeat 'community' composition are best explained by aridity. Specifically, EnSpm CACTA repeats were more abundant in palm species from wetter environments, which generally had larger genomes (>2.15Gbp/1C), suggesting amplification. In contrast, Ty1-copia Angela elements were more abundant in drier environments. Our results suggest water stress inhibits the expansion of repeats through selection on upper genome size limits. However, Ty1-copia Angela elements, which may associate with stress-response genes, have amplified in arid-adapted palm species. Overall, we provide novel evidence of climate influencing the assembly of repeat 'communities'.

Silencing of vitellogenin gene contributes to the promise of controlling red palm weevil, Rhynchophorus ferrugineus (Olivier)

Red palm weevil [Rhynchophorus ferrugineus (Olivier)], is native to South Asia and expanding its distribution range globally. Recent invasions of red palm weevil around the world, including Saudi Arabia, has become a global constraint for the production of palm species. Although, several control measures have been tested, none of them seemed successful against this invasive species. Therefore, we focused on silencing the reproduction control gene vitellogenin (Vg) based on RNA interference (RNAi) strategy for its possible application in the management of R. ferrugineus. The Vg is a major yolk protein precursor critical for oogenesis. To do this, fat body transcriptome of R. ferrugineus female adults was sequenced, which provided partial Vg gene transcript (FPKM 5731.60). A complete RfVg gene transcript of 5504 bp encoding 1787 amino acids was then sequenced using RCAE-PCR strategy and characterized. Phylogenetic analysis suggested that RfVg has closer ancestry to the coleopteran insects. The RfVg-based RNAi significantly suppressed the expressions of Vg gene. The 15, 20 and 25 days post-injection periods suppressed Vg expressions by 95, 96.6 and 99%, respectively. The suppressed Vg expressions resulted in the dramatic failure of Vg protein expression, which caused atrophied ovaries or no oogenesis and ultimately eggs were not hatched. These results suggest that knockdown of Vg gene involved in R. ferrugineus reproduction is a promising target for RNAi-based management of R. ferrugineus.