scholarly journals Analysis of weighted co-regulatory networks in maize provides insights into new genes and regulatory mechanisms related to inositol phosphate metabolism

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Shaojun Zhang ◽  
Wenzhu Yang ◽  
Qianqian Zhao ◽  
Xiaojin Zhou ◽  
Ling Jiang ◽  
...  
1997 ◽  
Vol 130 (1-2) ◽  
pp. 131-139 ◽  
Author(s):  
Simon F Vroemen ◽  
Wil J.A Van Marrewijk ◽  
Jeroen De Meijer ◽  
Aloys Th.M Van den Broek ◽  
Dick J Van der Horst

2020 ◽  
Author(s):  
Danye Qiu ◽  
Miranda S. Wilson ◽  
Verena B. Eisenbeis ◽  
Robert K. Harmel ◽  
Esther Riemer ◽  
...  

AbstractThe analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is highly desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables for the first time the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [13C6]-myo-inositol or [13C6]-D-glucose. In doing so, we uncover that there must be unknown inositol synthesis pathways in mammals, highlighting the unique potential of this method to dissect inositol phosphate metabolism and signalling.


1989 ◽  
Vol 141 (3) ◽  
pp. 606-617 ◽  
Author(s):  
Fernando A. Gonzalez ◽  
Ramona G. Alfonzo ◽  
Jorge R. Toro ◽  
Leon A. Heppel

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