scholarly journals Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Qin Zhao ◽  
Sha Xie ◽  
Yani Sun ◽  
Yiyang Chen ◽  
Jiming Gao ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Avian Hepatitis E Virus

A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus

2006 ◽  
Vol 131 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Narayanan Jothikumar ◽  
Theresa L. Cromeans ◽  
Betty H. Robertson ◽  
X.J. Meng ◽  
Vincent R. Hill
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Sensitive Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

scholarly journals First Report of Hepatitis E Virus in Shellfish in Southeast Italy

2020 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Gianfranco La Bella ◽  
Maria Grazia Basanisi ◽  
Gaia Nobili ◽  
Valentina Terio ◽  
Elisabetta Suffredini ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Potential Role ◽  
Hepatitis E ◽  
Developed Countries ◽  
Rt Pcr ◽  
Viral Pathogens ◽  
Apulia Region ◽  
Causative Agents

Hepatitis E virus (HEV) represents one of the principal causative agents of hepatitis globally. Among the five HEV genotypes affecting humans, genotypes 3 and 4 are zoonotic and are the main source of hepatitis E in developed countries. HEV has been detected in several foods. The present work investigated the presence of this virus in shellfish sold at retail in the Apulia region of Italy. The presence of HEV RNA was assessed by real-time RT-PCR in 225 shellfish samples collected during 2018. Overall, two (0.89%) of these samples tested positive for HEV RNA. To our knowledge, this is the first notification of the detection of HEV in mussels sold at retail in the Apulia region. These data highlight the potential role of shellfish as a vehicle for the transmission of viral pathogens.

scholarly journals Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

2015 ◽  
Vol 11 (1) ◽  
pp. 65 ◽  
Author(s):  
Carinne Puech ◽  
Laurence Dedieu ◽  
Isabelle Chantal ◽  
Valérie Rodrigues
Keyword(s):  
Real Time ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Sheep And Goats

scholarly journals Validation of single real-time TaqMan® PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens

2006 ◽  
Vol 78 (8) ◽  
pp. 1076-1082 ◽  
Author(s):  
V. Enouf ◽  
G. Dos Reis ◽  
J.P. Guthmann ◽  
P.J. Guerin ◽  
M. Caron ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pcr Assay ◽  
Clinical Specimens

Comparison of real-time fluorescent RT-PCR and conventional RT-PCR for the detection of hepatitis E virus genotypes prevalent in China

2007 ◽  
Vol 79 (12) ◽  
pp. 1966-1973 ◽  
Author(s):  
Chenyan Zhao ◽  
Zhuo Li ◽  
Baoshan Yan ◽  
Tim J. Harrison ◽  
Xinhui Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Rt Pcr ◽  
Virus Genotypes

Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR

2010 ◽  
Vol 139 (3) ◽  
pp. 206-209 ◽  
Author(s):  
Danielle Leblanc ◽  
Elyse Poitras ◽  
Marie-Josée Gagné ◽  
Pierre Ward ◽  
Alain Houde
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Rt Pcr ◽  
Virus Load

scholarly journals Diagnosis of Barmah Forest Virus Infection by a Nested Real-Time SYBR Green RT-PCR Assay

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e65197 ◽  
Author(s):  
Linda Hueston ◽  
Cheryl S. Toi ◽  
Neisha Jeoffreys ◽  
Tania Sorrell ◽  
Gwendolyn Gilbert
Keyword(s):  
Virus Infection ◽  
Real Time ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay
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