scholarly journals Plasmodium falciparum multidrug resistance gene-1 polymorphisms in Northern Nigeria: implications for the continued use of artemether-lumefantrine in the region

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Auwal Adamu ◽  
Mahmoud Suleiman Jada ◽  
Hauwa Mohammed Sani Haruna ◽  
Bassa Obed Yakubu ◽  
Mohammed Auwal Ibrahim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Multidrug Resistance ◽  
Epidemiological Data ◽  
Northern Nigeria ◽  
Multidrug Resistance Gene ◽  
Chi Square ◽  
Cycle Sequencing ◽  
North West ◽  
The North

Abstract Background The analysis of single nucleotide polymorphism (SNPs) in drug-resistance associated genes is a commonly used strategy for the surveillance of anti-malarial drug resistance in populations of parasites. The present study was designed and performed to provide genetic epidemiological data of the prevalence of N86Y-Y184F-D1246Y SNPs in Plasmodium falciparum multidrug resistance 1 (pfmdr1) in the malaria hotspot of Northern Nigeria. Methods Plasmodium falciparum-positive blood samples on Whatman-3MM filter papers were collected from 750 symptomatic patients from four states (Kano, Kaduna, Yobe and Adamawa) in Northern Nigeria, and genotyped via BigDye (v3.1) terminator cycle sequencing for the presence of three SNPs in pfmdr1. SNPs in pfmdr1 were used to construct NYD, NYY, NFY, NFD, YYY, YYD, YFD and YFY haplotypes, and all data were analysed using Pearson Chi square and Fisher’s exact (FE) tests. Results The prevalence of the pfmdr1 86Y allele was highest in Kaduna (12.50%, 2 = 10.50, P = 0.02), whilst the 184F allele was highest in Kano (73.10%, 2 = 13.20, P = 0.00), and the pfmdr1 1246Y allele was highest in Yobe (5.26%, 2 = 9.20, P = 0.03). The NFD haplotype had the highest prevalence of 69.81% in Kano (2 = 36.10, P = 0.00), followed by NYD with a prevalence of 49.00% in Adamawa, then YFD with prevalence of 11.46% in Kaduna. The YYY haplotype was not observed in any of the studied states. Conclusion The present study suggests that strains of P. falciparum with reduced sensitivity to the lumefantrine component of AL exist in Northern Nigeria and predominate in the North-West region.

scholarly journals Plasmodium falciparum Multidrug Resistance Gene-1 N86Y-Y184F-D1246Y Polymorphisms in Northern Nigeria: Implications for the continued use of artemether-lumefantrine in the region

2020 ◽  
Author(s):  
Auwal Adamu ◽  
Mahmoud Suleiman Jada ◽  
Hauwa Mohammed Sani Haruna ◽  
Bassa Obed Yakubu ◽  
Mohammed Auwal Ibrahim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Multidrug Resistance ◽  
Epidemiological Data ◽  
Northern Nigeria ◽  
Multidrug Resistance Gene ◽  
Chi Square ◽  
Cycle Sequencing ◽  
North West ◽  
The North

Abstract Background: The analysis of single nucleotide polymorphism (SNPs) in drug-resistance associated genes is a commonly used strategy for the surveillance of antimalarial drug resistance in populations of parasites. The present study was designed and performed to provide genetic epidemiological data of the prevalence of N86Y-Y184F-D1246Y SNPs in Plasmodium falciparum multidrug resistance 1 (pfmdr1) in the malaria hotspot of Northern Nigeria.Methods: Plasmodium falciparum-positive blood samples on Whatman-3MM filter papers were collected from 750 symptomatic patients from four states (Kano, Kaduna, Yobe and Adamawa) in Northern Nigeria, and genotyped via BigDye (v3.1) terminator cycle sequencing for the presence of three SNPs in pfmdr1. SNPs in pfmdr1 were used to construct NYD, NYY, NFY, NFD, YYY, YYD, YFD and YFY haplotypes, and all data were analyzed using Pearson Chi-square and Fisher's exact (FE) tests.Results: The prevalence of the pfmdr1 86Y allele was highest in Kaduna (12.50%, 𝜒² = 10.50, P = 0.02), whilst the 184F allele was highest in Kano (73.10%, 𝜒² = 13.20, P = 0.00), and the pfmdr1 1246Y allele was highest in Yobe (5.26%, 𝜒² = 9.20, P = 0.03). The NFD haplotype had the highest prevalence of 69.81% in Kano (𝜒² = 36.10, P = 0.00), followed by NYD with a prevalence of 49.00% in Adamawa, then YFD with prevalence of 11.46% in Kaduna. The YYY haplotype was not observed in any of the studied states.Conclusion: The present study shows that P. falciparum strains with potential reduced susceptibility to the lumefantrine component of AL exist in Northern Nigeria and predominate in the North-West region.

scholarly journals Plasmodium falciparum Multidrug Resistance Gene-1 polymorphisms in Northern Nigeria: implications for the continued use of artemether-lumefantrine in the region

2020 ◽  
Author(s):  
Auwal Adamu ◽  
Mahmoud Suleiman Jada ◽  
Hauwa Mohammed Sani Haruna ◽  
Bassa Obed Yakubu ◽  
Mohammed Auwal Ibrahim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Multidrug Resistance ◽  
Epidemiological Data ◽  
Northern Nigeria ◽  
Multidrug Resistance Gene ◽  
Chi Square ◽  
Cycle Sequencing ◽  
North West ◽  
The North

Abstract Background The analysis of single nucleotide polymorphism (SNPs) in drug-resistance associated genes is a commonly used strategy for the surveillance of anti-malarial drug resistance in populations of parasites. The present study was designed and performed to provide genetic epidemiological data of the prevalence of N86Y-Y184F-D1246Y SNPs in Plasmodium falciparum multidrug resistance 1 (pfmdr1) in the malaria hotspot of Northern Nigeria.Methods Plasmodium falciparum-positive blood samples on Whatman-3MM filter papers were collected from 750 symptomatic patients from four states (Kano, Kaduna, Yobe and Adamawa) in Northern Nigeria, and genotyped via BigDye (v3.1) terminator cycle sequencing for the presence of three SNPs in pfmdr1. SNPs in pfmdr1 were used to construct NYD, NYY, NFY, NFD, YYY, YYD, YFD and YFY haplotypes, and all data were analysed using Pearson Chi-square and Fisher's exact (FE) tests.Results The prevalence of the pfmdr1 86Y allele was highest in Kaduna (12.50%, 𝜒² = 10.50, P = 0.02), whilst the 184F allele was highest in Kano (73.10%, 𝜒² = 13.20, P = 0.00), and the pfmdr1 1246Y allele was highest in Yobe (5.26%, 𝜒² = 9.20, P = 0.03). The NFD haplotype had the highest prevalence of 69.81% in Kano (𝜒² = 36.10, P = 0.00), followed by NYD with a prevalence of 49.00% in Adamawa, then YFD with prevalence of 11.46% in Kaduna. The YYY haplotype was not observed in any of the studied states.Conclusion The present study suggests that strains of P. falciparum with reduced sensitivity to the lumefantrine component of AL exist in Northern Nigeria and predominate in the North-West region.

scholarly journals Plasmodium Falciparum Multidrug Resistance Gene-1 N86Y-Y184F-D1246Y Polymorphisms in Northern Nigeria: Implications for the Continued Use of Artemether-Lumefantrine in the Region

2020 ◽  
Author(s):  
Auwal Adamu ◽  
Mahmoud Suleiman Jada ◽  
Hauwa Mohammed Sani Haruna ◽  
Bassa Obed Yakubu ◽  
Mohammed Auwal Ibrahim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Multidrug Resistance ◽  
Epidemiological Data ◽  
Northern Nigeria ◽  
Multidrug Resistance Gene ◽  
Chi Square ◽  
Cycle Sequencing ◽  
North West ◽  
The North

Abstract Background The analysis of single nucleotide polymorphism (SNPs) in drug-resistance associated genes is a commonly used strategy for the surveillance of antimalarial drug resistance in populations of parasites. The present study was designed and performed to provide genetic epidemiological data of the prevalence of N86Y-Y184F-D1246Y SNPs in Plasmodium falciparum multidrug resistance 1 (pfmdr1) in the malaria hotspot of Northern Nigeria. Methods Plasmodium falciparum-positive blood samples on Whatman-3MM filter papers were collected from 750 symptomatic patients from four states (Kano, Kaduna, Yobe and Adamawa) in Northern Nigeria, and genotyped via BigDye (v3.1) terminator cycle sequencing for the presence of three SNPs in pfmdr1. SNPs in pfmdr1 were used to construct NYD, NYY, NFY, NFD, YYY, YYD, YFD and YFY haplotypes, and all data were analyzed using Pearson Chi-square and Fisher's exact (FE) tests. Results The prevalence of the pfmdr1 86Y allele was highest in Kaduna (12.5%, 𝜒² = 10.47, P < 0.05), whilst the 184F allele was highest in Kano (73.1%, 𝜒² = 13.20, P < 0.05), and the pfmdr1 1246Y allele was highest in Yobe (5.26%, 𝜒² = 9.18, P < 0.05). The NFD haplotype had the highest prevalence of 69.81% in Kano (𝜒² = 36.05, P < 0.05), followed by NYD with a prevalence of 49% in Adamawa, then YFD with prevalence of 11.46% in Kaduna. The YYY haplotype was not observed in any of the studied states. Conclusion The present study shows that P. falciparum strains with reduced sensitivity to artemether-lumefantrine exist in Northern Nigeria and predominate in the North-West region.

Studies on antimalarial drug susceptibility in Colombia, in relation to Pfmdr1 and Pfcrt

Parasitology ◽  
2008 ◽  
Vol 135 (5) ◽  
pp. 547-553 ◽  
Author(s):  
E. RESTREPO-PINEDA ◽  
E. ARANGO ◽  
A. MAESTRE ◽  
V. E. DO ROSÁRIO ◽  
P. CRAVO
Keyword(s):  
Drug Resistance ◽  
Drug Susceptibility ◽  
Snp Analysis ◽  
North West ◽  
The North ◽  
West Region ◽  
Health Authorities ◽  
Pcr Rflp ◽  
Alternative Drugs

SUMMARYIn Colombia, Plasmodium resistance to antimalarials such as chloroquine and antifolates is a serious problem. As a result, the national Colombian health authorities are monitoring the efficacy of alternative drugs and schemes. The study of genetic polymorphisms related with drug resistance is required in the region. In vitro responses to chloroquine, quinine, mefloquine, amodiaquine, desethylamodiaquine, artesunate and dihydroartesunate were carried out by HRP ELISA. SNP analysis in Pfcrt and Pfmdr1 genes was performed by PCR-RFLP in 77 samples from the North West region of Colombia. In vitro resistance to chloroquine was high (74%), followed by mefloquine (30%) and desethylamodiaquine (30%). A positive correlation between the IC50 of paired drugs was also detected. The allele Pfmdr1 N86 (wild) was present in 100% of the samples and 1246Y (mutant) in 92%. However, their presence did not correlate with in vitro drug resistance. Presence of the mutations K76T and N75E in Pfcrt was confirmed in all samples. Analysis of 4 codons (72, 74, 75 and 76) in pfcrt confirmed the presence of the haplotypes CMET in 91% and SMET in 9% of the samples.

Pesticide Use Practices by Market Gardeners in the Santa Area of the North West Region of Cameroon

2019 ◽  
pp. 1-11
Author(s):  
A. N. Abdulai ◽  
C. N. Konje ◽  
Tange Denis Achiri ◽  
D. N. Tarla ◽  
D. Nsobinenyui
Keyword(s):  
Crop Production ◽  
Insect Pest ◽  
Vegetable Production ◽  
Chi Square ◽  
North West ◽  
Pests And Diseases ◽  
Agronomic Practices ◽  
The North ◽  
Important Pest ◽  
Chi Square Test

Aims: This work aimed at establishing the different Garden crop pests and how farmers use different pesticides to manage these pests. Place and duration of the Work: This work was carried out in Santa from January to March 2013 Methodology: The methodology involved the use of structured questionnaires which were administered to 120 farmers to obtain information on demographics, constraints to successful crop production, general farm system and agronomic practices, knowledge of insect pest problems, types of pesticides used, dosage and rate of application, and different pests crops suffered from. Data was analysed using descriptive statistics and chi-square test. Results: The study indicates that majority of market gardeners in Santa community are males (70%) with ages from 30-39 and most of them are married (76%). Famers worked with relied on pesticides for pest control and did not dispose of empty pesticide containers properly such as throwing on the farm; use as drinking bowls and returned it to the pesticide vendors. Conclusion: This study provides valuable information on the pesticides used in pests and diseases control in vegetable production and health symptoms like stomach disorders, burns, and catarrh experienced by gardeners. A majority (94%) of farmers suffered from serious insect pest attacks on their farms and the most important pest was the cutworm (Agrotis ipsilon) which affected (65%). A majority (96%) of farmers also reported diseases attacks with late blight (Phytophthora infestans) affecting more than four-fifth (81%) of the farms especially in the rainy season.

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