Protein signature characterizing Helicobacter pylori strains of patients with autoimmune atrophic gastritis, duodenal ulcer and gastric cancer

Autoimmune atrophic gastritis (AAG) is associated with an increased risk of certain types of gastric cancer (GC). Helicobacter pylori (H. pylori) infection may have a role in the induction and/or maintenance of AAG and GC. Toll-like receptors (TLR) are essential for H. pylori recognition and subsequent innate and adaptive immunity responses. This study therefore aimed to characterize TLR polymorphisms, and features of bacterial flagellin A in samples from patients with AAG (n = 67), GC (n = 114) and healthy donors (HD; n = 97). TLR5 rs5744174 C/C genotype was associated with GC, lower IgG anti H. pylori response and a higher H. pylori flagellin A abundance and motility. In a subset of patients with AAG, H. pylori strains showed a reduction of the flagellin A abundance and a moderate motility compared with strains from GC patients, a prerequisite for active colonization of the deeper layers of the mucosa, host immune response and inflammation. TLR9 rs5743836 T allele showed an association with serum gastrin G17. In conclusion, our study suggests that alterations of flaA protein, moderate motility in H. pylori and two polymorphisms in TLR5 and TLR9 may favor the onset of AAG and GC, at least in a subset of patients. These findings corroborate the function of pathogen–host cell interactions and responses, likely influencing the pathogenetic process.

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Relationship between Helicobacter pylori , atrophic gastritis and gastric cancer

Alimentary Pharmacology & Therapeutics ◽

10.1111/j.1365-2036.1998.00009.x ◽

1998 ◽

Vol 12 ◽

pp. 25-36 ◽

Cited By ~ 71

Author(s):

E.J. Kuipers

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Atrophic Gastritis

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Proteomics signature of autoimmune atrophic gastritis: towards a link with gastric cancer

Gastric Cancer ◽

10.1007/s10120-020-01148-3 ◽

2021 ◽

Author(s):

Ombretta Repetto ◽

Valli De Re ◽

Paolo Giuffrida ◽

Marco Vincenzo Lenti ◽

Raffaella Magris ◽

...

Keyword(s):

Gastric Cancer ◽

Atrophic Gastritis ◽

Tricarboxylic Acid Cycle ◽

Differential Abundance ◽

Expression Levels ◽

2D Dige ◽

Gastric Corpus ◽

Gastric Biopsies ◽

H Pylori ◽

Autoimmune Atrophic Gastritis

Abstract Background Autoimmune atrophic gastritis (AAG) is a chronic disease that can progress to gastric cancer (GC). To better understand AAG pathology, this proteomics study investigated gastric proteins whose expression levels are altered in this disease and also in GC. Methods Using two-dimensional difference gel electrophoresis (2D-DIGE), we compared protein maps of gastric corpus biopsies from AAG patients and controls. Differentially abundant spots (|fold change|≥ 1.5, P < 0.01) were selected and identified by LC–MS/MS. The spots were further assessed in gastric antrum biopsies from AAG patients (without and with Helicobacter pylori infection) and from GC patients and unaffected first-degree relatives of GC patients. Results 2D-DIGE identified 67 differentially abundant spots, with 28 more and 39 less abundant in AAG-corpus than controls. LC–MS/MS identified these as 53 distinct proteins. The most significant (adjusted P < 0.01) biological process associated with the less abundant proteins was “tricarboxylic acid cycle”. Of the 67 spots, 57 were similarly differentially abundant in AAG-antrum biopsies irrespective of H. pylori infection status. The differential abundance was also observed in GC biopsies for 14 of 28 more abundant and 35 of 39 less abundant spots, and in normal gastric biopsies of relatives of GC patients for 6 and 25 spots, respectively. Immunoblotting confirmed the different expression levels of two more abundant proteins (PDIA3, GSTP gene products) and four less abundant proteins (ATP5F1A, PGA3, SDHB, PGC). Conclusion This study identified a proteomics signature of AAG. Many differential proteins were shared by GC and may be involved in the progression of AAG to GC.

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Helicobacter pyloriAnti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Canadian Journal of Gastroenterology ◽

10.1155/2002/589087 ◽

2002 ◽

Vol 16 (8) ◽

pp. 527-532 ◽

Cited By ~ 13

Author(s):

M Fatih Abasiyanik ◽

Ersan Sander ◽

Barik A Salih

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Chronic Gastritis ◽

Molecular Weights ◽

Cancer Antigens ◽

Gastroduodenal Disease ◽

H Pylori ◽

Asymptomatic Subjects

BACKGROUND: Several reports have shown the prevalence of anti-CagA antibodies to be associated with the development of peptic ulcer diseases, while others have indicated that there is no such association.AIM: To examine the prevalence of antibodies to CagA and otherHelicobacter pyloriantigens in symptomatic and asymptomatic subjects in Turkey.SUBJECTS AND METHODS: Sixty-six symptomatic subjects, 16 to 74 years of age, were examined forH pyloriby biopsy-based tests and ELISA. One hundred nineteen asymptomatic subjects, 20 to 65 years of age, were also tested serologically for the presence ofH pylori. Samples from both groups that were found to be positive forH pyloriby ELISA were then tested by immunoblotting.RESULTS: Fifty-four (82%) symptomatic subjects and 76 (64%) asymptomatic subjects were found to beH pylori-positive by ELISA. Samples from 30 symptomatic subjects who were found to beH pylori-positive by ELISA were analyzed by immunoblotting. Antibodies to CagA (116 kDa) antigen were detected in immunoblots of 11 of 14 (79%) with chronic gastritis, 12 of 13 (92%) with duodenal ulcer and three of three (100%) with gastric cancer. Antigens of the following molecular weights were also detected in these 30 subjects: 89 kDa (VacA) in 21 (70%), 37 kDa in 21 (70%), 35 kDa in 19 (63%), 30 kDa in 27 (90%) and 19.5 kDa in 19 (63%). Immunoblots of 40 ELISA-positive asymptomatic subjects showed that 33 (83%) had antibodies to CagA antigen, 26 (65%) to VacA antigen, 30 (75%) to a 37 kDa antigen, 30 (75%) to a 35 kDa antigen, 39 (98%) to a 30 kDa antigen and 36 (90%) to a 19.5 kDa antigen.CONCLUSIONS: Antibodies to CagA antigen were prevalent in both groups, regardless of the presence of gastroduodenal disease.

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Restriction Fragment Length Polymorphism in Helicobacter pylori Associated with Gastric Cancer and Duodenal Ulcer

Bioscience and Microflora ◽

10.12938/bifidus1996.17.61 ◽

1998 ◽

Vol 17 (1) ◽

pp. 61-64 ◽

Cited By ~ 1

Author(s):

Koichi KIMURA ◽

Shunji HAYASHI ◽

Hiroshi ISOGAI ◽

Emiko ISOGAI ◽

Toshiro SUGIYAMA ◽

...

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Restriction Fragment Length Polymorphism ◽

Restriction Fragment ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Restriction Fragment Length

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Dietary intake of walnut prevented Helicobacter pylori-associated gastric cancer through rejuvenation of chronic atrophic gastritis

Journal of Clinical Biochemistry and Nutrition ◽

10.3164/jcbn.20-103 ◽

2021 ◽

Vol 68 (1) ◽

pp. 37-50

Author(s):

Jong Min Park ◽

Young Min Han ◽

Yong Jin Park ◽

Ki Baik Hahm

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Dietary Intake ◽

Atrophic Gastritis ◽

Chronic Atrophic Gastritis

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VacA and CagA Status as Biomarker of Two Opposite End Outcomes of Helicobacter pylori Infection (Gastric Cancer and Duodenal Ulcer) in a Moroccan Population

PLoS ONE ◽

10.1371/journal.pone.0170616 ◽

2017 ◽

Vol 12 (1) ◽

pp. e0170616 ◽

Cited By ~ 12

Author(s):

Mounia El Khadir ◽

Samia Alaoui Boukhris ◽

Dafr-Allah Benajah ◽

Karima El Rhazi ◽

Sidi Adil Ibrahimi ◽

...

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Helicobacter Pylori Infection ◽

Moroccan Population ◽

Caga Status

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Intracellular VacA Is a Valuable Marker to Predict Whether Helicobacter pylori Induces Progressive Atrophic Gastritis That Is Associated with the Development of Gastric Cancer

Digestive Diseases and Sciences ◽

10.1007/s10620-005-1278-9 ◽

2005 ◽

Vol 50 (1) ◽

pp. 56-64 ◽

Cited By ~ 3

Author(s):

Mikio Karita ◽

Satoshi Teramukai ◽

Satoru Matsumoto ◽

Hidemi Shibuta

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Atrophic Gastritis ◽

Valuable Marker ◽

Development Of Gastric Cancer

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Two Cases of White Globe Appearance in Autoimmune Atrophic Gastritis

Case Reports in Gastrointestinal Medicine ◽

10.1155/2018/7091520 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5

Author(s):

Masaya Iwamuro ◽

Takehiro Tanaka ◽

Hiromitsu Kanzaki ◽

Seiji Kawano ◽

Yoshiro Kawahara ◽

...

Keyword(s):

Gastric Cancer ◽

Atrophic Gastritis ◽

Parietal Cells ◽

Microscopic Features ◽

Autoimmune Atrophic Gastritis ◽

Necrotic Debris

In this report, we described two patients with white globe appearance in autoimmune atrophic gastritis. Endoscopy revealed multiple white substances in the stomach in both cases. Biopsied specimens from the lesions contained dilated glands and showed a decrease in parietal cells. Intraglandular necrotic debris and carcinoma were absent. These results confirmed that white globe appearance can be observed in autoimmune atrophic gastritis. Moreover, microscopic features for white globe appearance observed in these cases were different from those reported previously in gastric cancer lesions and were similar to those observed for noncancerous stomach.

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