Alginate-chitosan core-shell microcapsule cultures of hepatic cells in a small scale stirred bioreactor: impact of shear forces and microcapsule core composition

AbstractA small scale stirred bioreactor was designed and the effect of different agitation rates (30, 60 and 100 rpm) was investigated on HepG2 cells cultured in alginate-chitosan (AC) core-shell microcapsule in terms of the cell proliferation and liver-specific function. The microencapsulated hepatic cells could proliferate well when they were cultured for 10 days at 30 rpm while the cell-laden microcapsules showed no cell proliferation at 100 rpm in the bioreactor system. Albumin production rate, as an important liver function, increased also 1.8- and 1.5- fold under stirring rate of 30 rpm compared to the static culture and 60 rpm of agitation, respectively. Moreover, In comparison with the static culture, about 1.5-fold increment in urea production was observed at 30 rpm. Similarly, the highest expressions of albumin and P450 genes were found at 30 rpm stirring rate, which were 4.9- and 19.2-fold of the static culture. Addition of collagen to the microcapsule core composition (ACol/C) could improve the cell proliferation and functionality at 60 rpm in comparison with the cell-laden microcapsules without collagen. The study demonstrated the hepatic cell-laden ACol/C microcapsule hydrogel cultured in the small scale stirred bioreactor at low mixing rate has a great potential for mass production of the hepatic cells while maintaining liver-specific functions.

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Overexpression of miRNA-26a Increases Cell Proliferation in Hepatic Cells Via akt/Beta-Catenin Pathway

Journal of Clinical and Experimental Hepatology ◽

10.1016/j.jceh.2015.07.006 ◽

2015 ◽

Vol 5 ◽

pp. S3 ◽

Cited By ~ 1

Author(s):

Parul Gupta ◽

Satendra Kumar ◽

Pushpendra Sahu ◽

Senthil Venugopal

Keyword(s):

Cell Proliferation ◽

Beta Catenin ◽

Hepatic Cells

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Toughened Epoxy Adhesive Modified with Core-Shell Nanoparticles Containing Epoxy Groups on the Surface

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.184-185.1375 ◽

2012 ◽

Vol 184-185 ◽

pp. 1375-1379

Author(s):

Xu Gang Zhang ◽

Bin Zhang ◽

Ming Ming Sun ◽

Jian Hui Li ◽

Lei Wang ◽

...

Keyword(s):

Epoxy Resins ◽

Small Scale ◽

Core Shell ◽

Shell Nanoparticles ◽

Adhesion Properties ◽

Epoxy Networks ◽

Grinding Method ◽

Toughened Epoxy ◽

Core Shell Nanoparticles ◽

Modified Epoxy

The epoxy resins were toughened with reactive core-shell nanoparticles(CSNPs) with butyl acrylate (BA) as the core and methyl methacrylate (MMA) copolymerizing with glycidyl methacrylate (GMA) as the shell. The chemical structure of the CSNPs was characterized by FT-IR. The morphology of toughened epoxy networks were analyzed by SEM and TEM, and their adhesion properties were also detected. The results show that mixing methods and CSNP concentration have great influence on the morphology and adhesion properties of the toughened epoxy networks. CSNPs are uniformly dispersed in the epoxy resins by the grinding method. The modified epoxy networks obtained from the modified epoxy networks prepared by the grinding method(MEPN2) with 10 wt% CSNPs show the best adhesion properties, and the increase in maximum peel strength, 25°C sheer strength and 150°C sheer strength of the modified epoxy networks is 401.3% , 46.9% and 27.6% respectively over the unmodified epoxy networks due to the small-scale coagulations of CSNPs.

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Natural silk fibroin/polyaniline (core/shell) coaxial fiber: Fabrication and application for cell proliferation

Composites Science and Technology ◽

10.1016/j.compscitech.2013.01.008 ◽

2013 ◽

Vol 77 ◽

pp. 37-41 ◽

Cited By ~ 31

Author(s):

Youyi Xia ◽

Xiang Lu ◽

Hailiang Zhu

Keyword(s):

Cell Proliferation ◽

Silk Fibroin ◽

Core Shell ◽

Natural Silk ◽

Fiber Fabrication

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Memoirs: Changes in Chondriosomes Occurring in Pathological Conditions

Journal of Cell Science ◽

10.1242/jcs.s2-58.231.553 ◽

1913 ◽

Vol s2-58 (231) ◽

pp. 553-566

Author(s):

J. O. WAKELIN BARRATT

Keyword(s):

Cell Proliferation ◽

Normal Condition ◽

Hepatic Cells ◽

Large Size ◽

Pathological Conditions ◽

Pigment Granules ◽

Brownish Black ◽

Convoluted Tubules

(1) The mitochondria of hepatic cells in pigmeuted degeneration of the liver assume a brownish-black colour and form the pigment-granules characteristic of this condition. (2) In severe hæmoglobinæmia the chondriosomes of the cells of the convoluted tubules are more readily demonstrable than in the normal condition, their staining capacity being increased. In this condition the mitochondrial elements reach an abnormally large size and are observed to take part in the elimination of hæmoglobin. (3) In pathological conditions in which rapid cell proliferation is occurring, the chondriosomes of the prickle layer of the epidermis appear of large size and stain with unusual facility, details of their structure being readily observable. In this respect they contrast with normal epidermal chondriosomes, which stain imperfectly.

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Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study

Brazilian Journal of Medical and Biological Research ◽

10.1590/1414-431x20198026 ◽

2019 ◽

Vol 52 (5) ◽

Cited By ~ 2

Author(s):

C.C. Abreu ◽

T.N. Fernandes ◽

E.P. Henrique ◽

P.D.C. Pereira ◽

S.B. Marques ◽

...

Keyword(s):

Cell Proliferation ◽

Spatial Memory ◽

Exploratory Study ◽

Carassius Auratus ◽

Environmental Enrichment ◽

Small Scale

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Lens Epithelial Apoptosis and Cell Proliferation in Human Age-Related Cortical Cataract

European Journal of Ophthalmology ◽

10.1177/112067210501500206 ◽

2005 ◽

Vol 15 (2) ◽

pp. 213-220 ◽

Cited By ~ 27

Author(s):

A. Charakidas ◽

A. Kalogeraki ◽

M. Tsilimbaris ◽

P. Koukoulomatis ◽

D. Brouzas ◽

...

Keyword(s):

Cell Proliferation ◽

Cataract Extraction ◽

Fiber Formation ◽

Small Scale ◽

Specific Cell ◽

Extracapsular Cataract Extraction ◽

Cortical Cataract ◽

Age Related ◽

Anterior Lens Capsule ◽

Human Lenses

Purpose To probe the presence of apoptosis in the epithelium of human lenses with age-related cortical cataract as well as to assess cell proliferation, a predicted consequence of apoptotic cell death, in this specific cell population. Methods DNA fragmentation was assessed using terminal digoxigenin-labeled dUTP nick end labeling (TUNEL) in capsulotomy specimens obtained from patients who underwent either extracapsular cataract extraction for the removal of adult-onset cortical cataract (n=27) or clear lens extraction for the correction of high myopia (n=25). Cell proliferation was assayed in 23 epithelia of cataractous lenses, and 20 epithelia of non-cataractous lenses with the proliferation marker MIB1, a monoclonal antibody against the nuclear antigen Ki-67 that is detected throughout the cell cycle but is absent in the resting (G0) cell. Results TUNEL staining was observed in 25 (92.6%) specimens of cataractous lenses, whereas cells undergoing apoptosis were identified in 2 (8%) of the epithelia from non-catarac-tous lenses. Only two MIB1-positive samples were detected, one of which was a capsule obtained during intracapsular cataract extraction. Conclusions The epithelium of human lenses with cortical cataract undergoes low rate apoptotic death. This limited epithelial apoptosis is unlikely to result in any significant cell density decrease since epithelial gaps are likely to be replaced by cell proliferation at the germinative zone of the anterior lens capsule. Nevertheless, the accumulation of small-scale epithelial losses during lifetime may induce alterations in lens fiber formation and homeostasis and result in loss of lens transparency.

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Augmenter of liver regeneration enhances cell proliferation through the microRNA‐26a/Akt/cyclin D1 pathway in hepatic cells

Hepatology Research ◽

10.1111/hepr.13404 ◽

2019 ◽

Vol 49 (11) ◽

pp. 1341-1352 ◽

Cited By ~ 3

Author(s):

Parul Gupta ◽

Teja Naveen Sata ◽

Naushad Ahamad ◽

Rakibul Islam ◽

Ajay K. Yadav ◽

...

Keyword(s):

Cell Proliferation ◽

Liver Regeneration ◽

Cyclin D1 ◽

Hepatic Cells ◽

Augmenter Of Liver Regeneration

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Quantifying endothelial cell proliferation in the zebrafish embryo

F1000Research ◽

10.12688/f1000research.73130.1 ◽

2021 ◽

Vol 10 ◽

pp. 1032

Author(s):

George Bowley ◽

Timothy JA Chico ◽

Jovana Serbanovic-Canic ◽

Paul C Evans

Keyword(s):

Cell Proliferation ◽

Endothelial Cell ◽

Zebrafish Embryo ◽

Time Lapse ◽

Endothelial Cell Proliferation ◽

Small Scale ◽

Zebrafish Embryos ◽

Time Lapse Imaging

Introduction: Endothelial cell (EC) proliferation is a fundamental determinant of vascular development and homeostasis, and contributes to cardiovascular disease by increasing vascular permeability to blood-borne lipoproteins. Rodents have been traditionally used to analyse EC proliferation mechanisms in vascular health and disease; however, alternative models such as the zebrafish embryo allow researchers to conduct small scale screening studies in a physiologically relevant vasculature whilst reducing the use of mammals in biomedical research. In vitro models of EC proliferation are valuable but do not fully recapitulate the complexity of the in vivo situation. Several groups have used zebrafish embryos for vascular biology research because they offer the advantages of an in vivo model in terms of complexity but are also genetically manipulable and optically transparent. Methods: Here we investigated whether zebrafish embryos can provide a suitable model for the study of EC proliferation. We explored the use of antibody, DNA labelling, and time-lapse imaging approaches. Results: Antibody and DNA labelling approaches were of limited use in zebrafish due to the low rate of EC proliferation combined with the relatively narrow window of time in which they can label proliferating nuclei. By contrast, time-lapse imaging of fluorescent proteins localised to endothelial nuclei was a sensitive method to quantify EC proliferation in zebrafish embryos. Discussion: We conclude that time-lapse imaging is suitable for analysis of endothelial cell proliferation in zebrafish, and that this method is capable of capturing more instances of EC proliferation than immunostaining or cell labelling alternatives. This approach is relevant to anyone studying endothelial cell proliferation for screening genes or small molecules involved in EC proliferation. It offers greater biological relevance than existing in vitro models such as HUVECs culture, whilst reducing the overall number of animals used for this type of research.

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