Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China

AbstractExpanded newborn screening facilitates early identification and intervention of patients with inborn errors of metabolism (IEMs), There is a lack of disease spectrum data for many areas in China. To determine the disease spectrum and genetic characteristics of IEMs in Xi'an city of Shaanxi province in northwest China, 146152 newborns were screening by MSMS from January 2014 to December 2019 and 61 patients were referred to genetic analysis by next generation sequencing (NGS) and validated by Sanger sequencing. Seventy-five newborns and two mothers were diagnosed with IEMs, with an overall incidence of 1:1898 (1:1949 without mothers). There were 35 newborns with amino acidemias (45.45%, 1:4176), 28 newborns with organic acidurias (36.36%, 1:5220), and 12 newborns and two mothers with FAO disorders (18.18%; 1:10439 or 1:12179 without mothers). Phenylketonuria and methylmalonic acidemia were the two most common disorders, accounting for 65.33% (49/75) of all confirmed newborn. Some hotspot mutations were observed for several IEMs, including PAH gene c.728G>A for phenylketonuria; MMACHC gene c.609G>A and c.567dupT, MMUT gene c.323G>A for methylmalonic acidemia and SLC25A13 gene c.852_855del for citrin deficiency. Our study provides effective clinical guidance for the popularization and application of expanded newborn screening, genetic screening, and genetic counseling of IEMs in this region.

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First molecular detection of Babesia gibsoni in a horse

Equine Veterinary Education ◽

10.1111/eve.13555 ◽

2021 ◽

Author(s):

M. Karnik ◽

A. Kumar ◽

A. Shabir Khaki ◽

H. Mirza

Keyword(s):

Molecular Detection ◽

Babesia Gibsoni

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First Molecular Detection of Anaplasma platys and Coinfection with Babesia gibsoni in Dogs from Bengaluru, India

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2020.903.009 ◽

2020 ◽

Vol 9 (3) ◽

pp. 75-79

Author(s):

Medha Karnik ◽

Anjan Kumar ◽

M. Manjula ◽

H. D. Lohitha ◽

R. Narendra ◽

...

Keyword(s):

Molecular Detection ◽

Anaplasma Platys ◽

Babesia Gibsoni

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Mitochondrial cytochrome oxidase C subunit III (cox3) gene as a sensitive and specific target for molecular detection of Babesia gibsoni infection in dogs

Experimental Parasitology ◽

10.1016/j.exppara.2019.107771 ◽

2019 ◽

Vol 206 ◽

pp. 107771

Author(s):

Aishwarya A. Betgiri ◽

Sameer N. Jadhav ◽

Mrunalini Pawde ◽

Anurag Shukla ◽

Chandrashekhar Mote ◽

...

Keyword(s):

Cytochrome Oxidase ◽

Molecular Detection ◽

Mitochondrial Cytochrome ◽

Babesia Gibsoni ◽

Specific Target ◽

C Subunit ◽

Subunit Iii ◽

Mitochondrial Cytochrome Oxidase

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Clinicopathological findings, molecular detection and characterization of Babesia gibsoni infection in a sick dog from Italy

Veterinary Parasitology ◽

10.1016/j.vetpar.2009.07.022 ◽

2009 ◽

Vol 165 (3-4) ◽

pp. 318-322 ◽

Cited By ~ 17

Author(s):

M. Trotta ◽

E. Carli ◽

G. Novari ◽

T. Furlanello ◽

L. Solano-Gallego

Keyword(s):

Molecular Detection ◽

Babesia Gibsoni ◽

Clinicopathological Findings

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Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh

Infection Genetics and Evolution ◽

10.1016/j.meegid.2015.01.011 ◽

2015 ◽

Vol 31 ◽

pp. 53-60 ◽

Cited By ~ 11

Author(s):

Masashi Terao ◽

Shirin Akter ◽

Md. Golam Yasin ◽

Ryo Nakao ◽

Hirotomo Kato ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Detection ◽

Babesia Gibsoni

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First Molecular Detection of Babesia gibsoni in Stray Dogs from Thailand

Pathogens ◽

10.3390/pathogens10060639 ◽

2021 ◽

Vol 10 (6) ◽

pp. 639

Author(s):

Thom Do ◽

Ruttayaporn Ngasaman ◽

Vannarat Saechan ◽

Opal Pitaksakulrat ◽

Mingming Liu ◽

...

Keyword(s):

18S Rrna ◽

Molecular Detection ◽

Genetic Characterization ◽

18S Rrna Gene ◽

Rrna Gene ◽

Babesia Gibsoni ◽

Its1 Region ◽

Stray Dogs ◽

Southern Thailand ◽

Vector Borne

In southern Thailand, the increasingly growing population of stray dogs is a concern to public health and environmental safety because of the lack of medical attention and control. More importantly, these animals are considered reservoirs for many zoonotic pathogens. The objective of this study was to molecularly detect canine vector-borne pathogens, and to perform genetic characterization of Babesia gibsoni present in stray dogs from southern Thailand. Blood samples were collected from 174 stray dogs in two provinces (Songkhla and Narathiwat) in southern Thailand. PCR analyses were executed using specific primers based on the Babesia spp. 18S rRNA gene, Babesia gibsoni Internal transcribed spacer 1 (ITS1) region, Ehrlichia canis citrate synthase (gltA) gene, Hepatozoon spp. 18S rRNA gene and Anaplasma platys heat shock protein (groEL) gene. The most common canine vector-borne pathogen found infecting stray dogs in this study was Hepatozoon canis (24.7%) followed by A. platys (14.9%), Babesia vogeli (8.0%), B. gibsoni (6.3%), and E. canis (1.72%). Concurrent infection with more than one pathogen occurred in 72 cases. Phylogenetic analysis based on the ITS1 region and 18S rRNA gene revealed that the B. gibsoni isolates from this study shared a large proportion of their identities with each other and with other reported B. gibsoni genotypes from Asia. This study highlights the molecular detection of B. gibsoni in dogs in Thailand for the first time and presents the genetic characterization by sequencing the ITS1 region and 18S rRNA gene of B. gibsoni from Thailand. Follow-up studies are needed to elucidate the origin, distribution, and vectors of B. gibsoni parasites circulating in dogs in Thailand, as well as to determine to what extent dogs are important reservoir hosts for zoonotic canine vector-borne disease infection in the studied area.

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Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea

Pathogens ◽

10.3390/pathogens9020110 ◽

2020 ◽

Vol 9 (2) ◽

pp. 110

Author(s):

Min-Goo Seo ◽

In-Ohk Ouh ◽

Sang Kyu Lee ◽

Jong-Seok Lee ◽

Oh-Deog Kwon ◽

...

Keyword(s):

Nucleotide Sequence Identity ◽

Respiratory Diseases ◽

Molecular Detection ◽

Phylogenetic Analyses ◽

Economic Losses ◽

Equine Herpesvirus ◽

Genetic Characteristics ◽

Blood Samples ◽

Sequence Identity ◽

Nasal Swabs

Respiratory diseases cause significant economic losses (especially in the horse racing industry). The present study describes the detection and genetic characteristics of equine herpesvirus (EHV) from a total of 1497 samples from clinically healthy horses in Korea, including 926 blood samples, 187 lung tissues, and 384 nasal swabs. EHV-2 and EHV-5 were detected in 386 (41.7%; 95% CI: 38.5–44.9) and 201 (21.7%; 95% CI: 19.1–24.4) blood samples, respectively, and in 25 (13.4%; 95% CI: 8.5–18.2) and 35 (18.7%; 95% CI: 13.1–24.3) lung tissues, respectively. EHV-1 and EHV-4 were not detected in either blood or lung tissues. EHV-1, EHV-2, and EHV-5 were detected in 46 (12.0%; 95% CI: 8.7–15.2), 21 (5.5%; 95% CI: 3.2–7.7), and 43 (11.2%; 95% CI: 8.0–14.4) nasal swabs, respectively. EHV-4 was not detected in nasal swabs. Co-infection with EHV-2 and EHV-5 was detected in 11.6% (107/926) of the blood samples and 6.4% (12/187) of lung tissues. In nasal swabs, co-infection with EHV-1, EHV-2, and EHV-5 was detected in 0.8% (3/384) of samples. Phylogenetic analysis of the glycoprotein B gene showed that EHV-1, EHV-2, and EHV-5 strains demonstrated significant genetic diversity in Korea, with a nucleotide sequence identity among them that ranged from 95.7% to 100% for EHV-1, 96.2–100% for EHV-2, and 93.8–99.3% for EHV-5. These results are the first phylogenetic analyses of EHV-1 in Korea in nasal swabs from a nationwide population of clinically healthy horses. Both EHV-2 and EHV-5 from blood, lung tissues, and nasal swabs were also detected.

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Molecular detection of tick-borne haemopathogens in shelter dogs and Rhipicephalus sanguineus (sensu lato) ticks from Peninsular Malaysia

10.21203/rs.2.20162/v1 ◽

2020 ◽

Author(s):

Quincie Sipin ◽

Farina Mustaffa Kamal ◽

Malaika Watanabe ◽

Puteri Azaziah Megat Abdul Rani ◽

Nur Mahiza Md ◽

...

Keyword(s):

Molecular Detection ◽

Rhipicephalus Sanguineus ◽

Peninsular Malaysia ◽

Single Infection ◽

Babesia Gibsoni ◽

Blood Samples ◽

Babesia Vogeli ◽

Microscopic Evaluation ◽

Rhipicephalus Sanguineus Sensu Lato ◽

Shelter Dogs

Abstract Background Canine tick-borne haemopathogens (TBH) constitute a significant concern worldwide. The detection of these TBH is mainly achieved by microscopic evaluation, seroprevalence, and molecular detection. The present study was designed to investigate the molecular detection of Anaplasma platys , Babesia gibsoni, Babesia vogeli , and Ehrlichia canis in shelter dogs and Rhipicephalus sanguineus ( sensu lato ) ticks infesting them in Malaysia.Results A total of 220 blood samples and 140 ticks were collected from 10 animal shelters in Peninsular Malaysia. The presence of haemopathogens was detected using conventional PCR, sequenced, and identified at the species level. Of the 220 blood samples, 77 (35%) were positive with at least one of the four haemopathogens, of which E. canis predominated (20%) followed by B. gibsoni and B. vogeli both with detection of 7%, and A. platys (12%). In tick samples, 5 (3.57%) of the collected samples were positive with at least one of the three haemopathogens. Low detection of E. canis and A. platys were present (n = 2; 1.43%) as well as B. vogeli shows only 1 sample positive (0.71%) and no detection of B. gibsoni . For co-infection in dogs, single infection is common (24%), while co-infection with two haemopathogens (10%) was also observed. The occurrence of infection with three TBH was also observed in the sampled dogs (1%).Conclusion E. canis is the most common TBH affecting shelter dogs in Peninsular Malaysia. Co-infection is quite common and the most common co-infection present was E. canis and A. platys. The study highlighted the first extensive molecular detection of TBH in dogs and R. sanguineus ( sensu lato ) ticks in Malaysia

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