scholarly journals Purification and characterization of a novel ubiquitin-like antitumour protein with hemagglutinating and deoxyribonuclease activities from the edible mushroom Ramaria botrytis

AMB Express ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rong Zhou ◽  
Ya-Jie Han ◽  
Min-Hui Zhang ◽  
Ke-Ren Zhang ◽  
Tzi Bun Ng ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Purification And Characterization ◽  
Ramaria Botrytis

Purification and characterization of an N-acetyl-d-galactosamine-specific lectin from the edible mushroom Schizophyllum commune

2006 ◽  
Vol 1760 (3) ◽  
pp. 326-332 ◽  
Author(s):  
Podjana Chumkhunthod ◽  
Sureelak Rodtong ◽  
Stan J. Lambert ◽  
Anthony P. Fordham-Skelton ◽  
Pierre J. Rizkallah ◽  
...  
Keyword(s):  
Schizophyllum Commune ◽  
Edible Mushroom ◽  
Purification And Characterization

scholarly journals Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)

2008 ◽  
Vol 72 (12) ◽  
pp. 3107-3113 ◽  
Author(s):  
Kenji FUKUDA ◽  
Michika HIRAGA ◽  
Sadaki ASAKUMA ◽  
Ikichi ARAI ◽  
Mitsuo SEKIKAWA ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Edible Mushroom ◽  
Flammulina Velutipes ◽  
Purification And Characterization

Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides

2012 ◽  
Vol 50 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Ya-Jie Zou ◽  
He-Xiang Wang ◽  
Tzi-Bun Ng ◽  
Chen-Yang Huang ◽  
Jin-Xia Zhang
Keyword(s):  
Edible Mushroom ◽  
Purification And Characterization

Purification and characterization of a novel laccase from the edible mushroom Clitocybe maxima

2010 ◽  
Vol 45 (5) ◽  
pp. 627-633 ◽  
Author(s):  
Guo-Qing Zhang ◽  
Yi-Fan Wang ◽  
Xiao-Qing Zhang ◽  
Tzi Bun Ng ◽  
He-Xiang Wang
Keyword(s):  
Edible Mushroom ◽  
Purification And Characterization

scholarly journals Cloning, purification and characterization of trehalose-6-phosphate synthase fromPleurotus tuoliensis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5230 ◽  
Author(s):  
Xiangli Wu ◽  
Zhihao Hou ◽  
Chenyang Huang ◽  
Qiang Chen ◽  
Wei Gao ◽  
...  
Keyword(s):  
Sodium Citrate ◽  
Environmental Temperature ◽  
Edible Mushroom ◽  
Biochemical Properties ◽  
Metal Chelators ◽  
Purification And Characterization ◽  
Optimum Reaction ◽  
Inhibited Enzyme ◽  
Phosphate Synthase

Pleurotus tuoliensis, a kind of valuable and favorable edible mushroom in China, is always subjected to high environmental temperature during cultivation. In our previous study withP. tuoliensis, trehalose proved to be effective for tolerating heat stress. Trehalose-6-phosphate synthase (TPS; EC2.4.1.15) plays a key role in the biosynthesis of trehalose in fungi. In this study, a full-length of cDNA with 1,665 nucleotides encodingTPS(PtTPS) inP. tuoliensiswas cloned. The PtTPS amino acid was aligned with other homologues and several highly conserved regions were analyzed. Thus, the TPS protein was expressed inEscherichia coliand purified by affinity chromatography to test its biochemical properties. The molecular mass of the enzyme is about 60 kDa and the optimum reaction temperature and pH is 30 °C and 7, respectively. The UDP-glucose and glucose-6-phosphate were the optimum substrates among all the tested glucosyl donors and acceptors. Metal cations like Mg2+, Co2+, Mn2+, Ni2+, K+, Ag+stimulated PtTPS activity significantly. Metal chelators such as sodium citrate, citric acid, EDTA, EGTA and CDTA inhibited enzyme activity. Polyanions like heparin and chondroitin sulfate were shown to stimulate TPS activity.

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