Purification and Characterization of a Versatile Peroxidase from Edible Mushroom Pleurotus eryngii

2010 ◽  
Vol 18 (5) ◽  
pp. 824-829 ◽  
Author(s):  
Min CHEN ◽  
Shanjing YAO ◽  
Hong ZHANG ◽  
Xinle LIANG
Keyword(s):  
Edible Mushroom ◽  
Pleurotus Eryngii ◽  
Versatile Peroxidase ◽  
Purification And Characterization

Purification and characterization of an N-acetyl-d-galactosamine-specific lectin from the edible mushroom Schizophyllum commune

2006 ◽  
Vol 1760 (3) ◽  
pp. 326-332 ◽  
Author(s):  
Podjana Chumkhunthod ◽  
Sureelak Rodtong ◽  
Stan J. Lambert ◽  
Anthony P. Fordham-Skelton ◽  
Pierre J. Rizkallah ◽  
...  
Keyword(s):  
Schizophyllum Commune ◽  
Edible Mushroom ◽  
Purification And Characterization

scholarly journals Purification and characterization of versatile peroxidase from Lentinus squarrosulus and its application in biodegradation of lignocellulosics

2019 ◽  
Vol 6 (6) ◽  
pp. 280-286
Author(s):  
Aarthi Ravichandran ◽  
Ramya G Rao ◽  
Maheswarappa Gopinath ◽  
Manpal Sridhar
Keyword(s):  
Finger Millet ◽  
Nutritive Value ◽  
Crop Residues ◽  
Specific Activity ◽  
Gel Filtration ◽  
Versatile Peroxidase ◽  
Purification And Characterization ◽  
Optimum Ph ◽  
Km Value

Versatile Peroxidases are high redox potential peroxidases capable of degrading lignin of lignocellulosic crop residues. Hence Versatile Peroxidases are prominent biocatalysts in upgrading lignocellulosic biomass for biotechnological applications. In the interest of exploiting the potential of Versatile Peroxidase in improving the digestibility of crop residues through delignification, a novel Versatile Peroxidase was purified and characterized from the immobilized cultures of native isolate Lentinus squarrosulus. The enzyme was purified with a specific activity of 62 U/mg through ion exchange and gel filtration chromatographic procedures. The enzyme possessed high affinity towards RB5 and manganese with a Km value of 6.84 µM for RB5 and 0.15 mM for manganese. The optimum temperature for oxidation was identified to be 30°C and optimum pH for manganese and RB5 oxidation was 5 and 3 respectively. Reactivity of the enzyme towards diverse substrates was investigated besides studying the effect of metal ions and inhibitors on RB5 oxidation. The enhanced potential of this purified Versatile Peroxidase in biodegradation of crop residues was demonstrated through augmentation of digestibility of finger millet and paddy straws by 20%.The results demonstrated that Versatile Peroxidase from Lentinus squarrosulus is capable of enhancing the nutritive value of crop residues through delignification

Isolation and characterization of a novel two-component hemolysin, erylysin A and B, from an edible mushroom, Pleurotus eryngii

Toxicon ◽  
2010 ◽  
Vol 56 (8) ◽  
pp. 1436-1442 ◽  
Author(s):  
Taisei Shibata ◽  
Makiko Kudou ◽  
Yoshinobu Hoshi ◽  
Ayako Kudo ◽  
Naoki Nanashima ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Pleurotus Eryngii ◽  
Isolation And Characterization ◽  
Two Component

scholarly journals Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)

2008 ◽  
Vol 72 (12) ◽  
pp. 3107-3113 ◽  
Author(s):  
Kenji FUKUDA ◽  
Michika HIRAGA ◽  
Sadaki ASAKUMA ◽  
Ikichi ARAI ◽  
Mitsuo SEKIKAWA ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Edible Mushroom ◽  
Flammulina Velutipes ◽  
Purification And Characterization
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