Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation

Abstract Background Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Methods The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Results The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. The enhanced permeability through micropores was confirmed by Franz diffusion cell. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. In addition, the permeation rate was higher in pig skin than in human skin. Conclusions We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery.

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TheIn VitroPermeability Coefficient and Short-Term Absorption Rates for Vinyl Toluene Using Human Cadaver Skin Mounted in a Static Diffusion Cell Model

Drug and Chemical Toxicology ◽

10.1080/01480540500408614 ◽

2006 ◽

Vol 29 (1) ◽

pp. 39-55 ◽

Cited By ~ 4

Author(s):

William J. Fasano ◽

Kevin N. Baer

Keyword(s):

Cell Model ◽

Human Cadaver ◽

Diffusion Cell ◽

Short Term ◽

Human Cadaver Skin ◽

Cadaver Skin

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Use of Skin Substitutes in Adult Canadian Burn Centres

Canadian Journal of Plastic Surgery ◽

10.1177/229255039700500206 ◽

1997 ◽

Vol 5 (2) ◽

pp. 112-117 ◽

Cited By ~ 2

Author(s):

Richard A Hopper ◽

Judy Knighton ◽

Joel Fish ◽

Walter Peters

Keyword(s):

United States ◽

Donor Site ◽

The United States ◽

Human Cadaver ◽

Research Development ◽

Skin Substitutes ◽

Private Companies ◽

Pig Skin ◽

Human Cadaver Skin ◽

Cadaver Skin

Seventeen Canadian adult burn centres were surveyed to determine the pattern of use, cost and availability of nine skin substitutes. An equal number of centres in the United States with comparable bed capacities were approached for comparison. Eighty-eight per cent of the Canadian centres and 76% of the United States centres responded to the questionnaire. Human cadaver skin, pig skin and Biobrane were used by approximately twice as many United States centres as Canadian centres. Cultured epidermal autografts (CEAs) were used by 20% of the Canadian centres and 15% of the American centres. Opsite, Tegaderm and Duoderm were used widely in both countries. Alloderm was used only in the United States, and amnion was not used in either country. The most common use of each substitute varied among centres, however, the pattern of use was comparable between the two countries, with the exception that Biobrane was not used in Canada to cover donor site wounds. In the United States, 60% of cadaver skin and all CEAs were purchased from private companies, whereas use of these two relatively expensive skin substitutes in Canada was restricted to centres with access to hospital-affiliated skin banks or laboratories. With the dependence of Canadian centres on noncommercial sources of biological skin replacements, research development in established skin banks should be encouraged, and regional discrepancies regarding access to these facilities addressed.

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Transdermal delivery of metoprolol I: Comparison between hairless mouse and human cadaver skin and effect of n-decylmethyl sulfoxide

International Journal of Pharmaceutics ◽

10.1016/0378-5173(92)90337-2 ◽

1992 ◽

Vol 88 (1-3) ◽

pp. 391-396 ◽

Cited By ~ 17

Author(s):

Tapash K. Ghosh ◽

Muhammad J. Habib ◽

Knikki Childs ◽

Mariano Alexander

Keyword(s):

Transdermal Delivery ◽

Hairless Mouse ◽

Human Cadaver ◽

Human Cadaver Skin ◽

Cadaver Skin

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Transdermal Delivery of Narcotic Analgesics: Comparative Metabolism and Permeability of Human Cadaver Skin and Hairless Mouse Skin

Journal of Pharmaceutical Sciences ◽

10.1002/jps.2600831215 ◽

1994 ◽

Vol 83 (12) ◽

pp. 1723-1728 ◽

Cited By ~ 30

Author(s):

S.D. Roy ◽

S‐Y. E. Hou ◽

S.L. Witham ◽

G.L. Flynn

Keyword(s):

Transdermal Delivery ◽

Mouse Skin ◽

Hairless Mouse ◽

Human Cadaver ◽

Narcotic Analgesics ◽

Hairless Mouse Skin ◽

Comparative Metabolism ◽

Human Cadaver Skin ◽

Cadaver Skin

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Nanoemulsions as vehicles for transdermal delivery of glycyrrhizin

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502011000400014 ◽

2011 ◽

Vol 47 (4) ◽

pp. 769-778 ◽

Cited By ~ 17

Author(s):

Ranjit Kumar Harwansh ◽

Kartik Chandra Patra ◽

Surendra Kumar Pareta ◽

Jagadish Singh ◽

Mohammed Akhlaquer Rahman

Keyword(s):

Transdermal Delivery ◽

Isopropyl Alcohol ◽

Skin Permeation ◽

Skin Irritation ◽

Human Cadaver ◽

Permeation Enhancers ◽

Brij 35 ◽

Human Cadaver Skin ◽

Cadaver Skin ◽

Span 80

The present investigation aims to evaluate an isotropic and thermodynamically stable nanoemulsion formulation for transdermal delivery of glycyrrhizin (GZ), with minimum surfactant and cosurfactant (Smix) concentrations that could improve its solubility, permeation enhancement, and stability. Pseudo-ternary phase diagrams were developed and various nanoemulsion formulations were prepared using soyabean oil as oil, Span 80, Brij 35 as a surfactant and isopropyl alcohol as a cosurfactant. Nanoemulsion formulations that passed the thermodynamic stability tests were characterized for pH, viscosity and droplet size using a transmission electron microscopy. The transdermal ability of glycyrrhizin through human cadaver skin was determined using Franz diffusion cells. The in vitro skin permeation profile of the optimized nanoemulsion formulation (NE2) was compared to that of conventional gel. A significant increase in permeability parameters such as steady-state flux (Jss) and permeability coefficient (Kp) was observed in the optimized nanoemulsion formulation (NE2), which consisted of 1% wt/wt of mono ammonium glycyrrhizinate (MAG), 32.4% Span 80, 3.7% Brij 35, 10% isopropyl alcohol, 46.5% soyabean oil and 6.4% distilled water. No obvious skin irritation was observed for the studied nanoemulsion formulation (NE2) or the gel. The results indicated that nanoemulsions are promising vehicles for transdermal delivery of glycyrrhizin through human cadaver skin, without the use of additional permeation enhancers, because excipients of nanoemulsions act as permeation enhancers themselves.

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