Peyer's Patch Dendritic Cells Capturing Oral Antigen Interact with Antigen-Specific T Cells and Induce Gut-Homing CD4+CD25+Regulatory T Cells in Peyer's Patches

Polyclonal IgA secretion is inducible in murine B cells when DC-T from Peyer's patches (PP) provide the inducing stimulus. PP DC-T, which are composed predominantly of dendritic cells and Lyt-1+ T cells, are capable of dramatic augmentation of IgA secretion by PP or spleen B cells with minimal induction of IgM secretion. DC-T from spleen, however, are incapable of augmenting IgA secretion by either PP or spleen B cells. The level of IgA secretion is dependent upon the dose of DC-T providing the inducing stimulus and reaches a plateau with DC-T:B ratios of less than 1:1. This system for preferential induction of IgA responses should permit elucidation of cellular mechanisms involved in regulation of IgA secretion.

Download Full-text

Antigen-specific regulatory T cells are detected in Peyer's patches after the interaction between T cells and dendritic cells loaded with orally administered antigen

Immunobiology ◽

10.1016/j.imbio.2010.06.008 ◽

2011 ◽

Vol 216 (3) ◽

pp. 416-422 ◽

Cited By ~ 7

Author(s):

Katsuya Nagatani ◽

Yoshinori Komagata ◽

Kurumi Asako ◽

Maki Takayama ◽

Kazuhiko Yamamoto

Keyword(s):

T Cells ◽

Dendritic Cells ◽

Regulatory T Cells ◽

Peyer's Patches ◽

Peyer’S Patches

Download Full-text

Modulation of lymphocyte subsets in Peyer's patches of mice treated with monoclonal antibody against helper T-cells.

Journal of Histochemistry & Cytochemistry ◽

10.1177/36.4.2964470 ◽

1988 ◽

Vol 36 (4) ◽

pp. 417-423 ◽

Cited By ~ 3

Author(s):

T H Ermak ◽

H J Steger ◽

R L Owen ◽

M F Heyworth

Keyword(s):

Monoclonal Antibody ◽

T Cells ◽

Lymphocyte Subsets ◽

Germinal Centers ◽

Peyer's Patches ◽

Helper T Cells ◽

Size Difference ◽

Peyer’S Patches ◽

Peyer’S Patch ◽

Peyer's Patch

Treatment of mice with anti-L3T4, a monoclonal antibody directed against helper T-cells, impairs clearance of intestinal Giardia muris infection. The present study examined the effect of anti-L3T4 treatment on mouse Peyer's patch cytoarchitecture and on the distribution of T-cell subsets within microenvironments of the follicle. Female BALB/c mice, aged 8 weeks, were given 4-7 weekly injections of either anti-L3T4 (1 mg/wk) or PBS (control group), and Peyer's patches were examined by immunohistochemistry or flow cytometry. In anti-L3T4-treated mice, Peyer's patch follicles (B-cell areas) were about two thirds the size of follicles in controls, and virtually all the size difference occurred in germinal centers. Peyer's patches were depleted of L3T4+ cells, yet the proportion of Thy-1.2+ (all T) cells was not decreased correspondingly, and the distribution of Thy-1.2+ cells in the patches was similar to that in control mice. In anti-L3T4-treated mice, Thy-1.2+ cells consisted of (a) Ly-2+ (cytotoxic/suppressor T) cells, and (b) a population of Thy-1.2+ cells that were neither L3T4+ nor Ly-2+. After treatment, Ly-2+ cells accounted for most of the T-cells in interfollicular areas and were also scattered in follicles, in germinal centers, and below the dome epithelium--in areas where L3T4+ cells predominated in control mice. Thy-1.2+ cells that were L3T4- and Ly-2- were mainly localized below the dome epithelium. These shifts indicate complex interrelationships among different lymphocyte subsets in Peyer's patches.

Download Full-text

Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer's patches in an orally tolerized, collagen-induced arthritis mouse model

Arthritis Research & Therapy ◽

10.1186/ar2361 ◽

2008 ◽

Vol 10 (1) ◽

pp. R11 ◽

Cited By ~ 42

Author(s):

Min-Jung Park ◽

So-Youn Min ◽

Kyung-Su Park ◽

Young-Gyu Cho ◽

Mi-La Cho ◽

...

Keyword(s):

T Cells ◽

Dendritic Cells ◽

Regulatory T Cells ◽

Mouse Model ◽

Peyer's Patches ◽

Peyer’S Patches ◽

Collagen Induced Arthritis ◽

Indoleamine 2,3 Dioxygenase

Download Full-text

Functional characteristics of Peyer's patch cells. III. Carrier priming of T cells by antigen feeding.

Journal of Experimental Medicine ◽

10.1084/jem.142.6.1425 ◽

1975 ◽

Vol 142 (6) ◽

pp. 1425-1435 ◽

Cited By ~ 23

Author(s):

M F Kagnoff

Keyword(s):

T Cells ◽

B Cell ◽

Peyer's Patches ◽

Peyer’S Patches ◽

Peyer’S Patch ◽

Peyer's Patch ◽

Antibody Synthesis ◽

Humoral Antibody ◽

Cell Induction

Peyer's patch T cells may serve an important role in the interaction of the host with intraluminal gut antigens. Studies presented in this paper demonstrate that T cells in murine Peyer's patches can be carrier primed for helper function in the induction of an antihapten response by feeding antigen. Carrier priming was assessed by measuring the ability of Peyer's patch cells from mice fed heterologous erythrocytes to enhance an antitrinitrophenyl (TNP) response in vitro when added to normal Peyer's patch cells cultured with TNP coupled to the erythrocyte used for feeding. Priming of T helper cells in Peyer's patches was antigen specific and occurred when erythrocytes were administered orally but not when erythrocytes were injected intravenously or intraperitoneally. Murine Peyer's patches are naturally deficient in a cooperating accessory adherent cell type(s) required for B-cell induction to humoral antibody synthesis in vitro and antigen feeding does not result in significant induction of Peyer's patch B cells to humoral antibody synthesis in vivo. Since Peyer's patch T cells can be carrier-antigen primed for helper function in the absence of B-cell induction to humoral antibody synthesis, these studies may indicate that T-cell priming is less dependent than B-cell induction on cooperating accessory adherent cells.

Download Full-text

Faculty Opinions recommendation of Selective imprinting of gut-homing T cells by Peyer's patch dendritic cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1014346.194594 ◽

2003 ◽

Author(s):

Reina Mebius

Keyword(s):

T Cells ◽

Dendritic Cells ◽

Peyer’S Patch ◽

Peyer's Patch

Download Full-text

Faculty Opinions recommendation of Selective imprinting of gut-homing T cells by Peyer's patch dendritic cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1014346.193613 ◽

2003 ◽

Author(s):

Hans Acha-Orbea

Keyword(s):

T Cells ◽

Dendritic Cells ◽

Peyer’S Patch ◽

Peyer's Patch

Download Full-text

Peyer's Patch-Deficient Mice Demonstrate That Mycobacterium avium subsp. paratuberculosis Translocates across the Mucosal Barrier via both M Cells and Enterocytes but Has Inefficient Dissemination

Infection and Immunity ◽

10.1128/iai.01411-09 ◽

2010 ◽

Vol 78 (8) ◽

pp. 3570-3577 ◽

Cited By ~ 35

Author(s):

Luiz E. Bermudez ◽

Mary Petrofsky ◽

Sandra Sommer ◽

Raúl G. Barletta

Keyword(s):

Mycobacterium Avium ◽

Intestinal Mucosa ◽

Peyer's Patches ◽

Mucosal Barrier ◽

Peyer’S Patches ◽

M Cells ◽

Peyer’S Patch ◽

Peyer's Patch ◽

Mycobacterium Avium Subsp Paratuberculosis ◽

Deficient Mice

ABSTRACT Mycobacterium avium subsp. paratuberculosis, the agent of Johne's disease, infects ruminant hosts by translocation through the intestinal mucosa. A number of studies have suggested that M. avium subsp. paratuberculosis interacts with M cells in the Peyer's patches of the small intestine. The invasion of the intestinal mucosa by M. avium subsp. paratuberculosis and Mycobacterium avium subsp. hominissuis, a pathogen known to interact with intestinal cells, was compared. M. avium subsp. paratuberculosis was capable of invading the mucosa, but it was significantly less efficient at dissemination than M. avium subsp. hominissuis. B-cell knockout (KO) mice, which lack Peyer's patches, were used to demonstrate that M. avium subsp. paratuberculosis enters the intestinal mucosa through enterocytes in the absence of M cells. In addition, the results indicated that M. avium subsp. paratuberculosis had equal abilities to cross the mucosa in both Peyer's patch and non-Peyer's patch segments of normal mice. M. avium subsp. paratuberculosis was also shown to interact with epithelial cells by an α5β1 integrin-independent pathway. Upon translocation, dendritic cells ingest M. avium subsp. paratuberculosis, but this process does not lead to efficient dissemination of the infection. In summary, M. avium subsp. paratuberculosis interacts with the intestinal mucosa by crossing both Peyer's patches and non-Peyer's patch areas but does not translocate or disseminate efficiently.

Download Full-text

Secretory Immunoglobulin A Antibodies against the σ1 Outer Capsid Protein of Reovirus Type 1 Lang Prevent Infection of Mouse Peyer's Patches

Journal of Virology ◽

10.1128/jvi.78.2.947-957.2004 ◽

2004 ◽

Vol 78 (2) ◽

pp. 947-957 ◽

Cited By ~ 49

Author(s):

Amy B. Hutchings ◽

Anna Helander ◽

Katherine J. Silvey ◽

Kartik Chandran ◽

William T. Lucas ◽

...

Keyword(s):

Peyer's Patches ◽

Peyer’S Patches ◽

M Cells ◽

Peyer’S Patch ◽

Peyer's Patch ◽

Adult Mice ◽

Reovirus Type ◽

Outer Capsid

ABSTRACT Reovirus type 1 Lang (T1L) adheres to M cells in the follicle-associated epithelium of mouse intestine and exploits the transport activity of M cells to enter and infect the Peyer's patch mucosa. Adult mice that have previously cleared a reovirus T1L infection have virus-specific immunoglobulin G (IgG) in serum and IgA in secretions and are protected against reinfection. Our aim in this study was to determine whether secretory IgA is sufficient for protection of Peyer's patches against oral reovirus challenge and, if so, against which reovirus antigen(s) the IgA may be directed. Monoclonal antibodies (MAbs) of the IgA isotype, directed against the σ1 protein of reovirus T1L, the viral adhesin, were produced and tested along with other, existing IgA and IgG MAbs against reovirus T1L outer capsid proteins. Anti-σ1 IgA and IgG MAbs neutralized reovirus T1L in L cell plaque reduction assays and inhibited T1L adherence to L cells and Caco-2BBe intestinal epithelial cells in vitro, but MAbs against other proteins did not. Passive oral administration of anti-σ1 IgA and IgG MAbs prevented Peyer's patch infection in adult mice, but other MAbs did not. When anti-σ1 IgA and IgG MAbs were produced in mice from hybridoma backpack tumors, however, the IgA prevented Peyer's patch infection, but the IgG did not. The results provide evidence that neutralizing IgA antibodies specific for the σ1 protein are protective in vitro and in vivo and that the presence of these antibodies in intestinal secretions is sufficient for protection against entry of reovirus T1L into Peyer's patches.

Download Full-text