Hyperdiploidy and chromosomal rearrangements define the anaplastic variant of Wilms' tumor.

Flow cytometric measurement of the DNA content of Wilms' tumor cells revealed a striking correspondence with the histologic subtype and treatment outcome. In the 48 cases studied, a hyperdiploid DNA content ranging from 1.7 to 3.2 times the result for normal diploid cells distinguished all but one of the ten anaplastic tumors. Lower values, from 1.0 to 1.4 times the diploid DNA content, characterized the nonanaplastic specimens. By Kaplan-Meier analysis, the probability of achieving 3 years of relapse-free survival was significantly lower in the group with higher DNA content (0.42 v 0.87, P less than .01). Analysis of banded chromosomes for a subset of 22 patients contributed important information beyond the flow cytometric study. Cases of anaplasia associated with poorer responses to therapy showed numerous complex translocations, whereas all others lacked such changes. By combining flow cytometric techniques and conventional methods of chromosome analysis, it should be possible to identify those patients with Wilms' tumor who are most likely to fail therapy. The biologic implication of these findings is that the development of clinical drug resistance in Wilms' tumor is a result of the genetic instability of the malignant clone.

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Flow cytometric measurement of proliferation-associated nuclear antigen p105 and DNA content in immuno-proliferative small intestinal disease (IPSID)

Journal of Surgical Oncology ◽

10.1002/jso.2930580106 ◽

1995 ◽

Vol 58 (1) ◽

pp. 25-30 ◽

Cited By ~ 6

Author(s):

Taner Demirer ◽

Ozden Uzunalimoglu ◽

Tom Anderson ◽

Susan M. Koethe ◽

Patrick W. McFadden ◽

...

Keyword(s):

Dna Content ◽

Intestinal Disease ◽

Nuclear Antigen ◽

Flow Cytometric ◽

Flow Cytometric Measurement ◽

Small Intestinal ◽

Small Intestinal Disease

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Electrolytic degradation of DNA fluorochromes during flow cytometric measurement of electronic cell volume.

Journal of Histochemistry & Cytochemistry ◽

10.1177/28.4.6445379 ◽

1980 ◽

Vol 28 (4) ◽

pp. 330-334 ◽

Cited By ~ 3

Author(s):

O Alabaster ◽

D L Glaubiger ◽

V T Hamilton ◽

S A Bentley ◽

S E Shackney ◽

...

Keyword(s):

Cell Volume ◽

Dna Content ◽

Chemical Degradation ◽

Quantum Yields ◽

Light Scatter ◽

Electrolytic Production ◽

Flow Cytometric ◽

Chlorine Gas ◽

Electrode Polarity ◽

Flow Cytometric Measurement

Changes in flow cytometric measurement of DNA content can result from electrolytic chemical degradation of mithramycin, ethidium bromide, and propidium iodide during simultaneous measurement of electronic cell volume. Bench electrolysis also degrades these fluorochromes without changing the quantum yields, even when they are complexed to DNA. In the flow cytometer, electrolytic production of chlorine at the anode is the probable cause of this degradation, since exposure of these fluorochromes to chlorine gas produces the same effect. It is therefore advisable to measure the DNA content distribution alone before simultaneously measuring the DNA content and the electronic cell volume. If unavoidable effects on the DNA distribution are present, narrow forward-angle light scatter should be used as the cell size indicator during dual parameter measurements. Modifying instrument design by reversing electrode polarity might eliminate this problem.

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Abnormal DNA content in liver-cell dysplasia: A flow cytometric study

International Journal of Cancer ◽

10.1002/ijc.2910440203 ◽

1989 ◽

Vol 44 (2) ◽

pp. 204-207 ◽

Cited By ~ 27

Author(s):

M. Roncalli ◽

M. Borzio ◽

B. Brando ◽

G. Colloredo ◽

E. Servida

Keyword(s):

Liver Cell ◽

Dna Content ◽

Flow Cytometric ◽

Liver Cell Dysplasia ◽

Flow Cytometric Study

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Relationship of tumor-cell ploidy to histologic subtype and treatment outcome in children and adolescents with unresectable rhabdomyosarcoma.

Journal of Clinical Oncology ◽

10.1200/jco.1991.9.1.159 ◽

1991 ◽

Vol 9 (1) ◽

pp. 159-166 ◽

Cited By ~ 68

Author(s):

D N Shapiro ◽

D M Parham ◽

E C Douglass ◽

R Ashmun ◽

B L Webber ◽

...

Keyword(s):

Treatment Outcome ◽

Relative Risk ◽

Tumor Cell ◽

Dna Content ◽

Response To Therapy ◽

Disease Stage ◽

Anatomic Site ◽

Histologic Subtype ◽

Distinct Subgroup ◽

Diploid Cells

Clinical and histopathologic features are often inadequate for accurate prediction of relapse or survival of individual patients with rhabdomyosarcoma (RMS). We therefore studied the cellular DNA content (ploidy) of RMS cells in relation to histology and response to therapy in 37 patients with unresectable tumors. Using flow cytometric techniques, we found that about one third of patients had diploid tumor stem lines, regardless of the histologic subtype. In the group with abnormal ploidy, a hyperdiploid classification (1.10 to 1.80 times the DNA content of normal diploid cells) was exclusively associated with embryonal histology (P = .001). By contrast, near-tetraploidy (1.80 to 2.60 times the DNA content of normal cells) was strongly associated with alveolar histology (P = .001). Thus, in these histologic subtypes of RMS, abnormal ploidy appears to arise through different mechanisms. Tumor-cell ploidy had a significant impact on survival that was especially apparent in patients with unresectable, nonmetastatic (group III) tumors. In this subgroup, hyperdiploidy conferred the best prognosis and diploidy the worst (P less than .0001). None of the eight patients with diploid tumors survived for more than 18 months. Tumor-cell ploidy was the best predictor of treatment outcome for patients with either embryonal (P less than .001; relative risk, 25.5) or alveolar (P = .073; relative risk 7.1) RMS and contributed significantly after adjustment for disease stage and anatomic site. Patients with unresectable diploid RMS have an unacceptably high risk of treatment failure, justifying new therapeutic approaches for this distinct subgroup.

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