Interactions between Oral Bacteria and Antibacterial Polymer-Based Restorative Materials

Adherence of oral bacteria to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. The aim of this study was to investigate and compare the adherence of different restorative materials to Streptococcus mutans strain (CCUG35176) in order to ascertain possible differences. The materials tested ranged across different classes including: flowable composites (Gradia Direct LoFlo; Filtek Supreme XT Flowable), anterior composites (Gradia Direct Anterior), universal composites (Filtek Supreme XT), packable composites (Filtek Silorane; Filtek P60), glass-ionomers (Fuji IX Gp Extra; Equia) and a control reference material (Thermanox plastic coverlips). Bacterial suspension was deposited onto each material and the adhesion was evaluated trough the colony forming units (CFUs) determination. Packable silorane-based composite was found to be less adhesive than posterior packable composite P60, flowable composites and glass ionomers. The fluoride of glass ionomers did not prevent the attachment of S. mutans; furthermore, after roughness analysis and SEM investigations, the hypothesis that the difference in bacterial adhesion can be determined by the particular surface chemistry of the material itself as well as by different electrostatic forces between bacteria and restorative surfaces must be given serious consideration.

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The Modified Model Cavity Method for Assessing Antibacterial Properties of Dental Restorative Materials

Journal of Dental Research ◽

10.1177/00220345890680051701 ◽

1989 ◽

Vol 68 (5) ◽

pp. 835-839 ◽

Cited By ~ 14

Author(s):

S.D. Meryon ◽

S.G. Johnson

Keyword(s):

Antibacterial Activity ◽

Material Culture ◽

Antibacterial Properties ◽

Oral Bacteria ◽

Test Material ◽

Cavity Method ◽

Dental Restorative Materials ◽

Restorative Materials ◽

Dental Restorative

A new in vitro method for assessing the antibacterial properties of dental restorative materials is described with ratios of test material/ culture medium volume aiming to simulate conditions around a restoration in vivo. Antibacterial activity is determined by the reduction in optical density of the test culture relative to controls. The method was used for assessment of the antibacterial activity of five dental materials of different composition against five oral bacteria. Release of zinc and fluoride from these materials was also measured and correlated with antibacterial activity. There was a general trend toward greater antibacterial activity with increased zinc release, while fluoride release had a significant effect on only one organism. While all the materials, when freshly mixed, were strongly toxic to three out of the five bacteria studied, much of this activity was lost after the materials had set.

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Effect of Two Dental Restorative Materials on Adhesion and Molecular Structure of Oral Bacteria

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.18505 ◽

2020 ◽

Vol 20 (8) ◽

pp. 4643-4647

Author(s):

Shuai Xu ◽

Junfeng Guo ◽

Junjie Huang ◽

Gang Zhang ◽

Yinghui Tan

Keyword(s):

Molecular Structure ◽

Molecular Dynamics ◽

Molecular Dynamics Simulations ◽

Substrate Surface ◽

Oral Bacteria ◽

Oral Rehabilitation ◽

Dental Restorative Materials ◽

Restorative Materials ◽

Dynamics Simulations ◽

Dental Restorative

Dental restorative materials are widely used to repair teeth and dentition defects. However, the dental restorative materials tend to react with oral bacteria when they are exposed to oral conditions, which leads to a change in the oral microecology. Herein, we have employed molecular dynamics simulations to investigate the interaction between different dental restorative materials and oral bacteria. It was found that the staphylococcal protein A (SPA) is more likely to attach on the surface of silicon carbide (SiC) substrate than hematite (Fe2O3) substrate surface. Furthermore, the tightly adhesion and accumulation of SPA on SiC surface changes the molecular structure of SPA, which will induce a change in the oral microecology. This study has demonstrated that the adhesion and molecular structure of oral bacteria is strongly dependent on dental restorative materials by molecular dynamics simulations, and Fe2O3 is more suitable to be a dental restorative material. It is therefore believed that molecular dynamics simulations can be used to further screen suitable materials for oral rehabilitation.

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Faculty Opinions recommendation of Oral bacteria resistant to mercury and to antibiotics are present in children with no previous exposure to amalgam restorative materials.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1013898.192531 ◽

2003 ◽

Author(s):

Marilyn Roberts

Keyword(s):

Oral Bacteria ◽

Previous Exposure ◽

Restorative Materials

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Attachment of oral bacteria to titanium surfaces: An SEM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100170074 ◽

1994 ◽

Vol 52 ◽

pp. 468-469

Author(s):

J. E. Laffoon ◽

R. L. Anderson ◽

J. C. Keller ◽

C. D. Wu-Yuan

Keyword(s):

Technical Problem ◽

Titanium Implant ◽

Oral Bacteria ◽

Bacterial Cells ◽

Thin Sections ◽

Surface Ultrastructure ◽

Sem Study ◽

Key Factor ◽

Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

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