The Modified Model Cavity Method for Assessing Antibacterial Properties of Dental Restorative Materials

1989 ◽  
Vol 68 (5) ◽  
pp. 835-839 ◽  
Author(s):  
S.D. Meryon ◽  
S.G. Johnson
Keyword(s):  
Antibacterial Activity ◽  
Material Culture ◽  
Antibacterial Properties ◽  
Oral Bacteria ◽  
Test Material ◽  
Cavity Method ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Dental Restorative

A new in vitro method for assessing the antibacterial properties of dental restorative materials is described with ratios of test material/ culture medium volume aiming to simulate conditions around a restoration in vivo. Antibacterial activity is determined by the reduction in optical density of the test culture relative to controls. The method was used for assessment of the antibacterial activity of five dental materials of different composition against five oral bacteria. Release of zinc and fluoride from these materials was also measured and correlated with antibacterial activity. There was a general trend toward greater antibacterial activity with increased zinc release, while fluoride release had a significant effect on only one organism. While all the materials, when freshly mixed, were strongly toxic to three out of the five bacteria studied, much of this activity was lost after the materials had set.

Antibacterial Activity of Dental Restorative Materials

1971 ◽  
Vol 50 (2) ◽  
pp. 382-387 ◽  
Author(s):  
David M. Updegraff ◽  
Robert W.H. Chang ◽  
Richard W. Joos
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Lactobacillus Plantarum ◽  
Bacteriostatic Activity ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Mixed Bacteria ◽  
Dental Restorative

Twenty-one commercial dental restorative materials, aged under various conditions, were checked on two different culture mediums for their antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, a cariogenic streptococcus, and mixed bacteria from the mouth. The duration of bacteriostatic activity was decreased rapidly by leaching in water. Of the three acrylics checked for ability to prevent in vitro carieslike lesions, the acrylic with highest antibacterial activity had the least caries.

A Reproducibility Study of the Model Cavity Method for the In Vitro Toxicity Testing of Dental Restorative Materials

1984 ◽  
Vol 12 (4) ◽  
pp. 215-223
Author(s):  
Sylvia D. Meryon ◽  
Pat F. Uphill ◽  
Ann D. Cordery ◽  
Roger M. Browne
Keyword(s):  
Toxicity Testing ◽  
In Vitro Cytotoxicity ◽  
Research Centre ◽  
In Vitro Toxicity ◽  
Cavity Method ◽  
Dental Restorative Materials ◽  
Reproducibility Study ◽  
Restorative Materials ◽  
Dental Restorative

A reproducibility study of the model cavity system for evaluating the in vitro cytotoxicity of dental restorative materials was undertaken at Birmingham Dental School and Huntingdon Research Centre. Using a precise experimental protocol drawn up following liaison between the two centres, a number of materials were tested independently. Toxicity was assessed by measurement of reduction in fibroblast and macrophage numbers relative to controls. Overall reproducibility was good, and the ranking of the toxicity of the test materials by both centres was almost identical.

scholarly journals In Vitro Anti-Biofilm and Antibacterial Properties of Streptococcus downii sp. nov.

2021 ◽  
Vol 9 (2) ◽  
pp. 450
Author(s):  
Maigualida Cuenca ◽  
María Carmen Sánchez ◽  
Pedro Diz ◽  
Lucía Martínez-Lamas ◽  
Maximiliano Álvarez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Quantitative Polymerase Chain Reaction ◽  
Bacterial Load ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Oral Bacteria ◽  
Periodontal Pathogens ◽  
Biofilm Model ◽  
Biofilm Development

The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of Streptococcus downii sp. nov. To test anti-biofilm properties, Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were grown in a biofilm model in the presence or not of S. downii sp. nov. for up to 120 h. For the potential antibacterial activity, 24 h-biofilms were exposed to S. downii sp. nov for 24 and 48 h. Biofilms structures and bacterial viability were studied by microscopy, and the effect in bacterial load by quantitative polymerase chain reaction. A generalized linear model was constructed, and results were considered as statistically significant at p < 0.05. The presence of S. downii sp. nov. during biofilm development did not affect the structure of the community, but an anti-biofilm effect against S. mutans was observed (p < 0.001, after 96 and 120 h). For antibacterial activity, after 24 h of exposure to S. downii sp. nov., counts of S. mutans (p = 0.019) and A. actinomycetemcomitans (p = 0.020) were significantly reduced in well-structured biofilms. Although moderate, anti-biofilm and antibacterial activities of S. downii sp. nov. against oral bacteria, including some periodontal pathogens, were demonstrated in an in vitro biofilm model.

scholarly journals Antibacterial Properties of Nano-particles in Dental Restorative Materials: Literature Review

2020 ◽  
Vol 5 (10) ◽  
pp. 464-469
Author(s):  
Isra Aljubour ◽  
Ala Aljubour
Keyword(s):  
Literature Review ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Nano Particles ◽  
Current Evidence ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Almost All ◽  
Dental Restorative

Nanotechnology has been receiving a great importance because of its ability to modulate materials into their nano size. This has drastically led to many changes in chemical, physical and optical properties of almost all metals. The aim of the present literature review is to examine and quantitatively analyze the current evidence for the addition of different nanoparticles into dental restorative materials, to determine whether their incorporation increases the antibacterial/antimicrobial properties of the materials. The incorporation of nanoparticles into dental restorative materials was a favorable option we found during our review; the antibacterial activity of nanoparticle-modified dental materials was significantly higher compared with the original unmodified materials, TiO2 nanoparticles providing the greatest benefits. However, the high heterogeneity among the articles reviewed points to the need for further research and the application of standardized research protocols.

Effect of Two Dental Restorative Materials on Adhesion and Molecular Structure of Oral Bacteria

2020 ◽  
Vol 20 (8) ◽  
pp. 4643-4647
Author(s):  
Shuai Xu ◽  
Junfeng Guo ◽  
Junjie Huang ◽  
Gang Zhang ◽  
Yinghui Tan
Keyword(s):  
Molecular Structure ◽  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Substrate Surface ◽  
Oral Bacteria ◽  
Oral Rehabilitation ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Dynamics Simulations ◽  
Dental Restorative

Dental restorative materials are widely used to repair teeth and dentition defects. However, the dental restorative materials tend to react with oral bacteria when they are exposed to oral conditions, which leads to a change in the oral microecology. Herein, we have employed molecular dynamics simulations to investigate the interaction between different dental restorative materials and oral bacteria. It was found that the staphylococcal protein A (SPA) is more likely to attach on the surface of silicon carbide (SiC) substrate than hematite (Fe2O3) substrate surface. Furthermore, the tightly adhesion and accumulation of SPA on SiC surface changes the molecular structure of SPA, which will induce a change in the oral microecology. This study has demonstrated that the adhesion and molecular structure of oral bacteria is strongly dependent on dental restorative materials by molecular dynamics simulations, and Fe2O3 is more suitable to be a dental restorative material. It is therefore believed that molecular dynamics simulations can be used to further screen suitable materials for oral rehabilitation.

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