Kinetics of the Transient Phase or Pre-Steady-State Phase of Enzymic Reactions

2019 ◽  
pp. 377-412
Author(s):  
Stephen A. Kuby
Keyword(s):  
Steady State ◽  
Transient Phase ◽  
Kinetics Of

Kinetic analysis of mechanism of intestinal Na+-dependent sugar transport

1985 ◽  
Vol 248 (5) ◽  
pp. C498-C509 ◽  
Author(s):  
D. Restrepo ◽  
G. A. Kimmich
Keyword(s):  
Experimental Data ◽  
Steady State ◽  
Sugar Transport ◽  
Enzyme Kinetic ◽  
Steady State Distribution ◽  
State Distribution ◽  
Least Squares Fit ◽  
Coupling Stoichiometry ◽  
Rate Limiting ◽  
Kinetics Of

Zero-trans kinetics of Na+-sugar cotransport were investigated. Sugar influx was measured at various sodium and sugar concentrations in K+-loaded cells treated with rotenone and valinomycin. Sugar influx follows Michaelis-Menten kinetics as a function of sugar concentration but not as a function of Na+ concentration. Nine models with 1:1 or 2:1 sodium:sugar stoichiometry were considered. The flux equations for these models were solved assuming steady-state distribution of carrier forms and that translocation across the membrane is rate limiting. Classical enzyme kinetic methods and a least-squares fit of flux equations to the experimental data were used to assess the fit of the different models. Four models can be discarded on this basis. Of the remaining models, we discard two on the basis of the trans sodium dependence and the coupling stoichiometry [G. A. Kimmich and J. Randles, Am. J. Physiol. 247 (Cell Physiol. 16): C74-C82, 1984]. The remaining models are terter ordered mechanisms with sodium debinding first at the trans side. If transfer across the membrane is rate limiting, the binding order can be determined to be sodium:sugar:sodium.

scholarly journals Kinetics of Biofilm Detachment

1992 ◽  
Vol 26 (9-11) ◽  
pp. 1995-1998 ◽  
Author(s):  
B. M. Peyton ◽  
W. G. Characklis
Keyword(s):  
Steady State ◽  
Utilization Rate ◽  
Carbon Utilization ◽  
Biofilm Thickness ◽  
First Order ◽  
Detachment Rate ◽  
Biofilm Detachment ◽  
Biofilm Modeling ◽  
Kinetics Of ◽  
Sensitive Variable

In predictive biofilm modeling, the detachment rate coefficient may be the most sensitive variable affecting both the predicted rate and the extent of biofilm accumulation. At steady state the detachment rate must be equal to the net growth rate in the biofilm. In systems where organic carbon is growth-limiting, the substrate carbon utilization rate determines the net biomass production rate and, therefore, the steady state biomass detachment rate. Detachment rates, first order with biofilm thickness, fit the experimental data well, but are not predictive since the coefficients must be determined experimentally.

Sign in / Sign up

Export Citation Format

Share Document