Improvement of Mulberry (Morus spp.) by Protoplast Culture

Author(s):  
Gulab Khan Rohela ◽  
Pawan Shukla ◽  
Pawan Saini ◽  
Rajesh Kumar ◽  
Kunjupillai Vijayan ◽  
...  
Keyword(s):  
2005 ◽  
Vol 10 (4) ◽  
pp. 326-332 ◽  
Author(s):  
Young-Woong Shin ◽  
Seong-Kwon Lee ◽  
Yun-Ju Kwon ◽  
Soon-Jae Rhee ◽  
Sang-Won Choi

2017 ◽  
Vol 6 (2) ◽  
pp. 5256
Author(s):  
Daryoush Shafiei ◽  
Prof. Basavaiah*

In mulberry (Morus spp.), the process of selection of promising hybrids from F1 population requires the screening of a large number of progenies and a long period. To develop a simple and faster approach for screening, studies were conducted using F1 seeds of two crosses. The details of screening studies conducted in relation to seed-size and seedling-size are reported separately in two parts. In this part, the F1 seeds were size-graded as small, medium and large seeds; their progenies were raised separately and screened in nursery. There was a considerable degree of variation in size of seeds and medium-size class seeds were in high percentage in both the crosses. The length, width and weight of seeds were also varied between the seed size classes significantly in both the crosses. The seed size classes differ with high significance in shoot length and Root collar diameter and also differ significantly in root length and weight of seedlings. The positive correlation between the seed size and growth of seedlings, seed size and germination, seed size and seedling survival in nursery indicated that size-grading of seeds and rejection of small seeds in the beginning of screening process may help to increase the efficiency of screening by increasing the chances of getting superior hybrids from limited progenies. However, confirmation on the performance of large seedlings from small seed size class may help to draw conclusion. Hence, the studies are continued with size- grading of seedlings in the next part of screening study.


1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.


2020 ◽  
Vol 19 (2) ◽  
pp. 136-145
Author(s):  
Hare Krishna ◽  
Dhurendra Singh ◽  
Rama Shanker Singh ◽  
Lokesh Kumar ◽  
Brijesh Dutt Sharma ◽  
...  

1987 ◽  
Vol 6 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Phan V. Chuong ◽  
K. P. Pauls ◽  
W. D. Beversdorf

2004 ◽  
Vol 58 (4) ◽  
pp. 639-646 ◽  
Author(s):  
Magda Biasiolo ◽  
Maria Teresa Da Canal ◽  
Noemi Tornadore
Keyword(s):  

Plant Disease ◽  
2021 ◽  
Author(s):  
Gondi S Arunakumar ◽  
Belaghihalli Nanjappa Gnanesh ◽  
Haniyambadi B Manojkumar ◽  
Doss S. Gandhi ◽  
Mogili Thallapally ◽  
...  

Mulberry (Morus spp.) is an important crop in the sericulture industry as the leaves constitute the primary feed for the silkworm. The availability of diverse genetic sources of resistance to root- knot nematode (RKN; Meloidogyne spp.) are very scanty and therefore, a set of 415 varied exotic and indigenous germplasm accessions were screened under glasshouse conditions. Twenty one accessions were identified as highly resistant and 48 were resistant, the highest numbers of highly resistant/resistant accessions were found in Morus alba. Further, thirty accessions based on rooting ability were evaluated for field resistance at four different locations with infested soil. Finally, eight germplasm accessions; BR-8, Karanjtoli-1, Hosur-C8, Nagalur Estate, Tippu, Calabresa, Thai Pecah and SRDC-3 were identified as potential genetic sources in RKN resistance breeding programs or as resistant rootstock for the establishment of mulberry gardens. Sixteen SSR markers analyzed among the 77 resistant and susceptible accessions, generated 55 alleles, ranging from 2 to 5 with an average of 3.43 alleles per locus. Principle coordinate analysis grouped the accessions on the basis of RKN susceptible and resistant to a greater extent. The RKN susceptible accessions exhibited higher variability as compared to resistant accessions and they were more dispersed. Analysis of molecular variance showed that maximum molecular variance (78%) within the population and 22% between populations. Results of this study indicate that SSR markers are reliable for assessing genetic variability among the RKN resistant and susceptible mulberry accessions.


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