Generation and Analysis of Genetically Modified Mice

2021 ◽  
pp. 225-236
Author(s):  
Cord Brakebusch
Keyword(s):  
Genetically Modified ◽  
Genetically Modified Mice

Generating genetically modified mice using CRISPR/Cas-mediated genome engineering

2014 ◽  
Vol 9 (8) ◽  
pp. 1956-1968 ◽  
Author(s):  
Hui Yang ◽  
Haoyi Wang ◽  
Rudolf Jaenisch
Keyword(s):  
Genome Engineering ◽  
Genetically Modified ◽  
Genetically Modified Mice

Insights into the physiological role of WT1 from studies of genetically modified mice

2004 ◽  
Vol 16 (3) ◽  
pp. 287-300 ◽  
Author(s):  
Maria Teresa Discenza ◽  
Jerry Pelletier
Keyword(s):  
Target Genes ◽  
Transcriptional Regulatory Network ◽  
System Development ◽  
Genetically Modified ◽  
Physiological Role ◽  
Protein Isoforms ◽  
Urogenital System ◽  
Protein Partners ◽  
Genetically Modified Mice

Discenza, Maria Teresa, and Jerry Pelletier. Insights into the physiological role of WT1 from studies of genetically modified mice. Physiol Genomics 16: 287-300, 2004; 10.1152/physiolgenomics.00164.2003.—The identification of WT1 gene mutations in children with WAGR and Denys-Drash syndromes pointed toward a role for WT1 in genitourinary system development. Biochemical analysis of the different WT1 protein isoforms showed that WT1 is a transcription factor and also has the ability to bind RNA. Analysis of WT1 complexes identified several target genes and protein partners capable of interacting with WT1. Some of these studies placed WT1, its downstream targets, and protein partners in a transcriptional regulatory network that controls urogenital system development. We review herein studies on WT1 knockout and transgenic models that have been instrumental in defining a physiological role for WT1 in normal and abnormal urogenital development.

scholarly journals Acid-sensing ion channels in sensory signaling

2020 ◽  
Vol 318 (3) ◽  
pp. F531-F543 ◽  
Author(s):  
Marcelo D. Carattino ◽  
Nicolas Montalbetti
Keyword(s):  
Nervous System ◽  
Ion Channels ◽  
Peripheral Nervous System ◽  
Sensory Neurons ◽  
Genetically Modified ◽  
Physiological Processes ◽  
Acid Sensing Ion Channels ◽  
Genetically Modified Mice ◽  
Sensory Processes

Acid-sensing ion channels (ASICs) are cation-permeable channels that in the periphery are primarily expressed in sensory neurons that innervate tissues and organs. Soon after the cloning of the ASIC subunits, almost 20 yr ago, investigators began to use genetically modified mice to assess the role of these channels in physiological processes. These studies provide critical insights about the participation of ASICs in sensory processes, including mechanotransduction, chemoreception, and nociception. Here, we provide an extensive assessment of these findings and discuss the current gaps in knowledge with regard to the functions of ASICs in the peripheral nervous system.

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