Diabetes mellitus-induced alterations of tissue optical properties, optical clearing efficiency, and molecular diffusivity

2021 ◽  
pp. 517-538
Daria K. Tuchina ◽  
Valery V. Tuchin
2010 ◽  
Vol 03 (03) ◽  
pp. 195-202 ◽  

Tissue optical clearing by use of optical clearing agents (OCAs) has been proven to have potential to reduce the highly scattering effect of biological tissues in optical techniques. However, the difference in tissue samples could lead to unreliable results, making it difficult to quantitatively control the dose of OCAs during the course of tissue optical clearing. In this work, in order to study the effects of optical clearing, we customized tissue-like phantoms with optical properties of some biological tissue. Diffuse reflectance and total transmittance of tissue-like phantoms with different OCAs (DMSO or glycerol) and porcine skin tissues were measured. Then optical property parameters were calculated by inverse adding-doubling (IAD) algorithm. Results showed that OCAs could lead to a reduction in scattering of tissue-like phantoms as it did to porcine skin tissue in vitro. Furthermore, a series of relational expressions could be fit to quantitatively describe the relationship between the doses of OCAs and the reduction of scattering effects. Therefore, proper tissue-like phantom could facilitate optical clearing to be used in quantitative control of tissue optical properties, and further promote the application potential of optical clearing to light-based noninvasive diagnostic and therapeutic techniques.

2010 ◽  
Vol 03 (03) ◽  
pp. 203-211 ◽  

Tissue Optical Clearing Devices (TOCDs) have been shown to increase light transmission through mechanically compressed regions of naturally turbid biological tissues. We hypothesize that zones of high compressive strain induced by TOCD pins produce localized water displacement and reversible changes in tissue optical properties. In this paper, we demonstrate a novel combined mechanical finite element model and optical Monte Carlo model which simulates TOCD pin compression of an ex vivo porcine skin sample and modified spatial photon fluence distributions within the tissue. Results of this simulation qualitatively suggest that light transmission through the skin can be significantly affected by changes in compressed tissue geometry as well as concurrent changes in tissue optical properties. The development of a comprehensive multi-domain model of TOCD application to tissues such as skin could ultimately be used as a framework for optimizing future design of TOCDs.

2018 ◽  
Vol 23 (09) ◽  
pp. 1 ◽  
Alexey N. Bashkatov ◽  
Kirill V. Berezin ◽  
Konstantin N. Dvoretskiy ◽  
Maria L. Chernavina ◽  
Elina A. Genina ◽  

2021 ◽  
Vol 11 (1) ◽  
Brett H. Hokr ◽  
Joel N. Bixler

AbstractDynamic, in vivo measurement of the optical properties of biological tissues is still an elusive and critically important problem. Here we develop a technique for inverting a Monte Carlo simulation to extract tissue optical properties from the statistical moments of the spatio-temporal response of the tissue by training a 5-layer fully connected neural network. We demonstrate the accuracy of the method across a very wide parameter space on a single homogeneous layer tissue model and demonstrate that the method is insensitive to parameter selection of the neural network model itself. Finally, we propose an experimental setup capable of measuring the required information in real time in an in vivo environment and demonstrate proof-of-concept level experimental results.

1997 ◽  
Vol 36 (1) ◽  
pp. 136 ◽  
Shao-Pow Lin ◽  
Lihong Wang ◽  
Steven L. Jacques ◽  
Frank K. Tittel

Sergey M. Zaytsev ◽  
Marine Amouroux ◽  
Grégoire Khairallah ◽  
Alexey N. Bashkatov ◽  
Valery V. Tuchin ◽  

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