QUANTITATIVE CONTROL OF OPTICAL CLEARING EFFECTS STUDIED WITH TISSUE-LIKE PHANTOM

2010 ◽  
Vol 03 (03) ◽  
pp. 195-202 ◽  
Author(s):  
JINGYING JIANG ◽  
WEI CHEN ◽  
QILIANG GONG ◽  
KEXIN XU
Keyword(s):  
Optical Properties ◽  
Biological Tissues ◽  
Porcine Skin ◽  
Tissue Samples ◽  
Optical Clearing ◽  
Tissue Optical Properties ◽  
Application Potential ◽  
The Difference ◽  
Tissue Optical Clearing

Tissue optical clearing by use of optical clearing agents (OCAs) has been proven to have potential to reduce the highly scattering effect of biological tissues in optical techniques. However, the difference in tissue samples could lead to unreliable results, making it difficult to quantitatively control the dose of OCAs during the course of tissue optical clearing. In this work, in order to study the effects of optical clearing, we customized tissue-like phantoms with optical properties of some biological tissue. Diffuse reflectance and total transmittance of tissue-like phantoms with different OCAs (DMSO or glycerol) and porcine skin tissues were measured. Then optical property parameters were calculated by inverse adding-doubling (IAD) algorithm. Results showed that OCAs could lead to a reduction in scattering of tissue-like phantoms as it did to porcine skin tissue in vitro. Furthermore, a series of relational expressions could be fit to quantitatively describe the relationship between the doses of OCAs and the reduction of scattering effects. Therefore, proper tissue-like phantom could facilitate optical clearing to be used in quantitative control of tissue optical properties, and further promote the application potential of optical clearing to light-based noninvasive diagnostic and therapeutic techniques.

scholarly journals PARAMETRIC STUDY OF TISSUE OPTICAL CLEARING BY LOCALIZED MECHANICAL COMPRESSION USING COMBINED FINITE ELEMENT AND MONTE CARLO SIMULATION

2010 ◽  
Vol 03 (03) ◽  
pp. 203-211 ◽  
Author(s):  
WILLIAM C. VOGT ◽  
HAIOU SHEN ◽  
GE WANG ◽  
CHRISTOPHER G. RYLANDER
Keyword(s):  
Finite Element ◽  
Monte Carlo ◽  
Optical Properties ◽  
Light Transmission ◽  
Compressive Strain ◽  
Monte Carlo Model ◽  
Biological Tissues ◽  
Optical Clearing ◽  
Tissue Optical Properties ◽  
Tissue Optical Clearing

Tissue Optical Clearing Devices (TOCDs) have been shown to increase light transmission through mechanically compressed regions of naturally turbid biological tissues. We hypothesize that zones of high compressive strain induced by TOCD pins produce localized water displacement and reversible changes in tissue optical properties. In this paper, we demonstrate a novel combined mechanical finite element model and optical Monte Carlo model which simulates TOCD pin compression of an ex vivo porcine skin sample and modified spatial photon fluence distributions within the tissue. Results of this simulation qualitatively suggest that light transmission through the skin can be significantly affected by changes in compressed tissue geometry as well as concurrent changes in tissue optical properties. The development of a comprehensive multi-domain model of TOCD application to tissues such as skin could ultimately be used as a framework for optimizing future design of TOCDs.

scholarly journals Мониторинг процесса иммерсионного оптического просветления коллагеновых волокон с помощью оптической когерентной томографии

2019 ◽  
Vol 127 (8) ◽  
pp. 337
Author(s):  
М.Е. Швачкина ◽  
Д.Д. Яковлев ◽  
Е.Н. Лазарева ◽  
А.Б. Правдин ◽  
Д.A. Яковлев
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Properties ◽  
Biological Tissues ◽  
Dehydration Process ◽  
Immersion Liquid ◽  
Reliable Technique ◽  
Optical Clearing ◽  
Osmotic Effect

AbstractFuture development of the method of immersion optical clearing of biological tissues—this method is widely used in the study of the morphology and pathologies of tissues in vitro and considered promising for in vivo applications in biophysical research and medicine—requires knowledge of the details of interaction of immersion liquids with the tissue, in particular, the characteristics both of the tissue dehydration process, which is caused by the osmotic effect of the immersion liquid, and the process of diffusion of the immersion agent (IA) into the tissue. The optical properties of skin dermis, eye sclera, tendon, and many other tissues are determined by the properties of collagen bundles, abundant in these tissues. In the present work, a convenient and reliable technique for monitoring the optical properties and geometry of collagen bundles in the course of their immersion clearing in vitro, based on optical coherence tomography (OCT), is proposed. The main advantage of this technique is that it allows one to monitor changes in the geometric and optical properties of the tissue simultaneously, without interrupting the natural course of the immersion clearing process, and to obtain reliable estimates of the characteristic times and rates of both the process of tissue dehydration and process of diffusion of the IA into the tissue.

scholarly journals Measurement of tissue optical properties in the context of tissue optical clearing

2018 ◽  
Vol 23 (09) ◽  
pp. 1 ◽  
Author(s):  
Alexey N. Bashkatov ◽  
Kirill V. Berezin ◽  
Konstantin N. Dvoretskiy ◽  
Maria L. Chernavina ◽  
Elina A. Genina ◽  
...  
Keyword(s):  
Optical Properties ◽  
Optical Clearing ◽  
Tissue Optical Properties ◽  
Tissue Optical Clearing

scholarly journals ASSESSMENT OF TISSUE OPTICAL CLEARING AS A FUNCTION OF GLUCOSE CONCENTRATION USING OPTICAL COHERENCE TOMOGRAPHY

2010 ◽  
Vol 03 (03) ◽  
pp. 169-176 ◽  
Author(s):  
NARENDRAN SUDHEENDRAN ◽  
MOHAMED MOHAMED ◽  
MOHAMAD G. GHOSN ◽  
VALERY V. TUCHIN ◽  
KIRILL V. LARIN
Keyword(s):  
Optical Coherence Tomography ◽  
Glucose Concentration ◽  
Glucose Solution ◽  
Tissue Sample ◽  
Biological Tissues ◽  
Optical Coherence ◽  
Porcine Skin ◽  
Optical Clearing ◽  
Reflected Light ◽  
Tissue Optical Clearing

One of the major challenges in imaging biological tissues using optical techniques, such as optical coherence tomography (OCT), is the lack of light penetration due to highly turbid structures within the tissue. Optical clearing techniques enable the biological samples to be more optically homogeneous, allowing for deeper penetration of light into the tissue. This study investigates the effect of optical clearing utilizing various concentrations of glucose solution (10%, 30%, and 50%) on porcine skin. A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time. The ratio of percentage clearing per tissue thickness for 10%, 30% and 50% glucose was determined to be 4.7 ±1.6% mm-1 (n = 6), 10.6 ±2.0% mm-1 (n = 7) and 21.8 ±2.2% mm-1 (n = 5), respectively. It was concluded that while higher glucose concentration has the highest optical clearing effect, a suitable concentration should be chosen for the purpose of clearing, considering the osmotic stress on the tissue sample.

scholarly journals Machine learning estimation of tissue optical properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Brett H. Hokr ◽  
Joel N. Bixler
Keyword(s):  
Neural Network ◽  
Optical Properties ◽  
Biological Tissues ◽  
Homogeneous Layer ◽  
In Vivo Measurement ◽  
Tissue Optical Properties ◽  
The Neural Network ◽  
Spatio Temporal ◽  
Fully Connected

AbstractDynamic, in vivo measurement of the optical properties of biological tissues is still an elusive and critically important problem. Here we develop a technique for inverting a Monte Carlo simulation to extract tissue optical properties from the statistical moments of the spatio-temporal response of the tissue by training a 5-layer fully connected neural network. We demonstrate the accuracy of the method across a very wide parameter space on a single homogeneous layer tissue model and demonstrate that the method is insensitive to parameter selection of the neural network model itself. Finally, we propose an experimental setup capable of measuring the required information in real time in an in vivo environment and demonstrate proof-of-concept level experimental results.

Measurement of the Optical Properties of Muscle Tissue In Vivo

2000 ◽  
Author(s):  
P. L. Kopsombut ◽  
D. Willis ◽  
A. E. Schen ◽  
L. X. Xu ◽  
X. Xu
Keyword(s):  
Optical Properties ◽  
Transport Theory ◽  
Rapid Development ◽  
Ccd Camera ◽  
High Sensitivity ◽  
Biological Tissues ◽  
In Vivo Measurement ◽  
Living Tissues

Abstract Along with rapid development of diagnostic and therapeutic applications of lasers in medicine, optical properties of various biological tissues have been extensively studied [1]. Most of the studies were performed in vitro owing to the complexity involved in in vivo measurement. To date, it is well understood that living tissue is an absorbing and scattering heterogeneous medium because of its complex structures including blood network. The transport theory cannot be readily used due to the heterogeneity and the absence of the optical properties of living tissues [2]. In this research, we have developed a procedure for measuring the total attenuation coefficient (μ1) of the exteriorized rat 2-D spinotrapezius muscle in the wavelength ranged from 480–560 nm using the collimated light from a Nitrogen-pumped dye laser and a high-sensitivity CCD camera.

ULTRASOUND ENHANCED SKIN OPTICAL CLEARING: MICROSTRUCTURAL CHANGES

2010 ◽  
Vol 03 (03) ◽  
pp. 189-194 ◽  
Author(s):  
XIANGQUN XU ◽  
CHAOJIE SUN
Keyword(s):  
Lipid Bilayers ◽  
Ex Vivo ◽  
Skin Surface ◽  
Glycerol Solution ◽  
Porcine Skin ◽  
Intact Skin ◽  
Sem Images ◽  
Optical Clearing ◽  
Better Than

Our previous studies demonstrated the ultrasound-induced skin optical clearing enhancement with topical application of optical clearing agents on in vitro porcine skin and in vivohuman skin. The objective of this study was to investigate the possible mechanisms of the enhanced skin optical clearing by ultrasound medications. Optical clearing effects of ex vivo guinea pig abdomen skin topically applied with 60% glycerol or the combination of 60% glycerol and ultrasound were studied by optical coherence tomography (OCT). Microstructure of skin surface was examined by scanning electron microscopy (SEM). Ultrasound with a frequency of 1 MHz and a power of 0.75 W over a 3-cm probe was simultaneously applied with glycerol solution for 15 min. The combination of 60% glycerol and ultrasound results in a 19% increase in OCT 1/e light penetration depth after 30 min, which is much better than 60% glycerol alone. SEM images demonstrated that changes in skin microstructure due to the tight order of the lipid bilayers in the stratum corneum disrupted and the separation of keratinocytes by the application of ultrasound contribute to the ultrasound-enhanced intact skin optical clearing effects.

Mechanical tissue optical clearing technique increases imaging resolution and contrast through Ex vivo porcine skin

2011 ◽  
Vol 43 (8) ◽  
pp. 814-823 ◽  
Author(s):  
Alondra Izquierdo-Román ◽  
William C. Vogt ◽  
Leeanna Hyacinth ◽  
Christopher G. Rylander
Keyword(s):  
Ex Vivo ◽  
Porcine Skin ◽  
Optical Clearing ◽  
Clearing Technique ◽  
Imaging Resolution ◽  
Tissue Optical Clearing ◽  
Mechanical Tissue

scholarly journals Измерение оптических свойств десны и дентина человека в спектральном диапазоне 350–800 нм

2020 ◽  
Vol 20 (4) ◽  
pp. 258-267
Author(s):  
Alexey Andreevich Selifonov ◽  
◽  
Olga Anatolyevna Zyuryukina ◽  
Ekaterina Nikolaevna Lazareva ◽  
Julia Sergeevna Skibina ◽  
...  
Keyword(s):  
Optical Properties ◽  
Spectral Range ◽  
Photothermal Therapy ◽  
Diffuse Reflection ◽  
Biological Tissues ◽  
Transmission Spectra ◽  
Tissue Samples ◽  
Scattering Coefficients ◽  
Total Transmission

Knowledge of the optical properties of biological tissues is important for the development of optical diagnostics, photodynamic and photothermal therapy of various diseases. However, despite the significant number of works devoted to the determination of the optical properties of tissues, the optical properties of human gums and dentin remain currently poorly understood. In this work, we experimentally studied the optical properties of human gums and dentin in the spectral range from 350 nm to 800 nm. Basing on measured diffuse reflection and total transmission spectra and using the Inverse Adding Doubling (IAD) method, the spectral dependences of absorption and scattering coefficients of the studied tissue samples were calculated.

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