Abstract
Objectives
Sprout of evening primrose (Oenothera laciniata, OL), Field horsetail (Equisetum arvense L., EAL), Tree of heaven (Ailanthus altissima, AA) leaves are all reported to be rich of flavonoids. This study was performed to determine the antioxidative effects of Sprout of evening primrose (Oenothera laciniata), Field horsetail (Equisetum arvense L.), Tree of heaven (Ailanthus altissima) leaves ethanol extracts in d-galactose induced aging rat model.
Methods
After 3weeks of adaptation period, 12-week-old SD rats were randomly divided into six groups (n = 5 each): Control group (C), D-galactose induced aging group (G), D-galactose injection with tomato treatment positive control group (PC), D-galactose injection with OL treatment group (O), D-galactose injection with EAL treatment group (E), D-galactose injection with AA treatment group (A). All groups except C group were intraperitoneally injected with D-galactose for 12 weeks and C group was treated with saline as a substitute.
Results
After 8 weeks of oral treatment period, there was no significant difference in body weight among six groups. Serum malondialdehyde (MDA) concentration levels increased significantly in G group compared to C group (P < 0.05). Serum advanced glycation end (AGE) concentration levels decreased significantly in O group and A group compared to G group (P < 0.05). Liver MDA level decreased significantly in O, E, A groups compared to G group (P < 0.05).
Conclusions
Sprout of evening primrose (Oenothera laciniata, OL), Tree of heaven (Ailanthus altissima, AA) extract consumption can ameliorate antioxidative activities by suppressing oxidative stress in d-galactose induced aging rat model. Further research is under progress to clarify the mechanism of antioxidative effects.
Funding Sources
This study was partly funded and cooperated by Ministry of Commerce Industry and Energy.