Use of In Situ Hybridization Histochemistry to Study Muscarinic Receptor mRNA Expression in Brains of Sleep-Deprived Rats

1997 ◽  
Author(s):  
Clete Kushida ◽  
Donna Simmons
Keyword(s):  
In Situ Hybridization ◽  
Mrna Expression ◽  
Muscarinic Receptor ◽  
Hybridization Histochemistry ◽  
Receptor Mrna ◽  
In Situ Hybridization Histochemistry

Localization of m5 muscarinic receptor mRNA in rat brain examined by in situ hybridization histochemistry

1990 ◽  
Vol 114 (2) ◽  
pp. 154-159 ◽  
Author(s):  
M.Teresa Vilaró ◽  
JoséM. Palacios ◽  
Guadalupe Mengod
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Muscarinic Receptor ◽  
Hybridization Histochemistry ◽  
Receptor Mrna ◽  
In Situ Hybridization Histochemistry

Expression of transforming growth factor alpha during development

1988 ◽  
Vol 66 (8) ◽  
pp. 1113-1121 ◽  
Author(s):  
V. K. M. Han ◽  
A. J. D'Ercole ◽  
D. C. Lee
Keyword(s):  
In Situ Hybridization ◽  
Growth Factor ◽  
Mrna Expression ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Egf Receptors ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Factor Alpha

Transforming growth factors (TGFs) are polypeptides that are produced by transformed and tumour cells, and that can confer phenotypic properties associated with transformation on normal cells in culture. One of these growth-regulating molecules, transforming growth factor alpha (TGF-α), is a 50 amino acid polypeptide that is related to epidermal growth factor (EGF) and binds to the EGF receptor. Previous studies have shown that TGF-α is expressed during rodent embryogenesis between 7 and 14 days gestation. To investigate the cellular sites of TGF-α mRNA expression during development, we have performed Northern analyses and in situ hybridization histochemistry on the conceptus and maternal tissues at various gestational ages. Contrary to previous reports, both Northern analyses and in situ hybridization histochemistry indicate that TGF-α mRNA is predominantly expressed in the maternal decidua and not in the embryo. Decidual expression is induced following implantation, peaks at day 8, and declines through day 15 when the decidua is being resorbed. In situ hybridization revealed that expression of TGF-α mRNA is highest in the region of decidua adjacent to the embryo and is low or nondetectable in the uterus, placenta, and embryo. In addition, we could not detect TGF-α mRNA expression in other maternal tissues, indicating that the induction of TGF-α transcripts in the decidua is tissue specific, and not a pleiotropic response to changes in hormonal milieu that occur during pregnancy. The developmentally regulated expression of TGF-α mRNA in the decidua, together with the presence of EGF receptors in this tissue, suggests that this peptide may stimulate mitosis and angiogenesis locally by an autocrine mechanism. Because EGF receptors are also present in the embryo and placenta, TGF-α may act on these tissues by a paracrine or endocrine mechanism.

Postnatal developmental changes in NSE and NNE mRNA expression in the rat pineal gland: In situ hybridization histochemistry

1998 ◽  
Vol 24 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Xue-Lian Feng ◽  
Hiroshi Usui ◽  
Toshiya Fujita ◽  
Tomio Ichikawa ◽  
Takashi Katagiri ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Pineal Gland ◽  
Mrna Expression ◽  
Developmental Changes ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Rat Pineal Gland

Expression of muscarinic and nicotinic receptor mRNA in the salivary gland of rats: a study by in situ hybridization histochemistry

1993 ◽  
Vol 17 (3-4) ◽  
pp. 335-339 ◽  
Author(s):  
Toru Shida ◽  
Atsushi Tokunaga ◽  
Eiji Kondo ◽  
Yutaka Ueda ◽  
Koji Ohno ◽  
...  
Keyword(s):  
Salivary Gland ◽  
In Situ Hybridization ◽  
Nicotinic Receptor ◽  
Hybridization Histochemistry ◽  
Receptor Mrna ◽  
In Situ Hybridization Histochemistry
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