Mechanisms of Laser-Induced Self-Organization of Nano- and Microstructures of Surface Relief in Air and in Liquid Environment

The bacterial cell is propelled through the liquid environment by means of one or more rotating flagella. The bacterial flagellum is composed of a basal body (rotary motor), hook (universal coupler), and filament (propellor). The filament is a rigid helical assembly of only one protein species — flagellin. The filament can adopt different morphologies and change, reversibly, its helical parameters (pitch and hand) as a function of mechanical stress and chemical changes (pH, ionic strength) in the environment.

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Beam-radiation effects on wool in the ESEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100144760 ◽

1986 ◽

Vol 44 ◽

pp. 674-675

Author(s):

G.D. Danilatos

Keyword(s):

Electron Beam ◽

Radiation Effects ◽

Research Work ◽

Beam Radiation ◽

Liquid Environment ◽

Know How ◽

Environmental Sem

The advent of the environmental SEM (ESEM) has made possible the examination of uncoated and untreated specimen surfaces in the presence of a gaseous or liquid environment. However, the question arises as to what degree the examined surface remains unaffected by the action of the electron beam. It is reasonable to assume that the beam invariably affects all specimens but the type and degree of effect may be totally unimportant for one class of applications and totally unacceptable for another; yet, for a third class, it is imperative to know how our observations are modified by the presence of the beam. The aim of this report is to create an awareness of the need to initiate research work in various fields in order to determine the guiding rules of the limitations (or even advantages) due to irradiation.

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X-ray Gabor holography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100139433 ◽

1995 ◽

Vol 53 ◽

pp. 612-613

Author(s):

Steve Lindaas ◽

Chris Jacobsen ◽

Alex Kalinovsky ◽

Malcolm Howells

Keyword(s):

Surface Relief ◽

Biological Imaging ◽

Specimen Preparation ◽

X Rays ◽

X Ray ◽

Water Window ◽

Biological Objects ◽

Transmission Imaging ◽

Atomic Force ◽

Electron Microscopes

Soft x-ray microscopy offers an approach to transmission imaging of wet, micron-thick biological objects at a resolution superior to that of optical microscopes and with less specimen preparation/manipulation than electron microscopes. Gabor holography has unique characteristics which make it particularly well suited for certain investigations: it requires no prefocussing, it is compatible with flash x-ray sources, and it is able to use the whole footprint of multimode sources. Our method serves to refine this technique in anticipation of the development of suitable flash sources (such as x-ray lasers) and to develop cryo capabilities with which to reduce specimen damage. Our primary emphasis has been on biological imaging so we use x-rays in the water window (between the Oxygen-K and Carbon-K absorption edges) with which we record holograms in vacuum or in air.The hologram is recorded on a high resolution recording medium; our work employs the photoresist poly(methylmethacrylate) (PMMA). Following resist “development” (solvent etching), a surface relief pattern is produced which an atomic force microscope is aptly suited to image.

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