Cl- Permeabilities in Red Blood Cells and Peripheral Blood Lymphocytes from Cystic Fibrosis and Control Subjects

Introduction: Opioid and amphetamine type stimulant (ATS) exert their rewarding effects by stimulating the dopaminergic system in the mesolimbic area. It has been suggested that dopamine system in peripheral blood lymphocytes reflect the central dopamine system’s activity and pathology, especially in neuropsychiatric diseases including drug addiction. The present study aimed to assess the effect of co-occurring opioid and ATS (COATS) addiction towards mRNA expression of dopamine receptors DRD4 and DRD5 in peripheral blood lymphocytes (PBLs) of drug dependent subjects (n=36) undergoing methadone maintenance therapy in comparison to control subjects (n=36). Materials and methods: Ten mL blood were obtained from the subjects followed by lymphocyte isolation, RNA extraction and reverse transcription. DRD4 and DRD5 mRNA expression in peripheral lymphocytes was assessed using real-time PCR. The DRD4 mRNA expression but not DRD5 was significantly reduced in the peripheral lymphocytes of COATS subjects. Results: Mean expression value for DRD4 was 14.0+0.24 among patients and 13.3+0.25 among control subjects. For DRD5 it was 12.87 + 0.75 among patients and 12.59 + 1.24 among controls. Conclusion: Inconclusion, co-occurring opioid and ATS addiction was associated with persistent deficiency of DRD4 but not DRD5 in PBLs. Bangladesh Journal of Medical Science Vol.20(3) 2021 p.563-568

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The Interleukin-2 Receptor Subunit Expression and Function on Peripheral Blood Lymphocytes from HIV-Infected and Control Persons

Clinical Immunology and Immunopathology ◽

10.1006/clin.1994.1052 ◽

1994 ◽

Vol 71 (1) ◽

pp. 60-68 ◽

Cited By ~ 15

Author(s):

G. Vanham ◽

L. Kestens ◽

J. Vingerhoets ◽

G. Penne ◽

R. Colebunders ◽

...

Keyword(s):

Peripheral Blood ◽

Interleukin 2 ◽

Peripheral Blood Lymphocytes ◽

Receptor Subunit ◽

Blood Lymphocytes ◽

Interleukin 2 Receptor ◽

Subunit Expression ◽

And Function ◽

And Control

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Chromosomal damage in peripheral blood lymphocytes from nurses occupationally exposed to chemicals

Human & Experimental Toxicology ◽

10.1177/0960327113512338 ◽

2013 ◽

Vol 33 (9) ◽

pp. 897-903 ◽

Cited By ~ 10

Author(s):

A Santovito ◽

P Cervella ◽

M Delpero

Keyword(s):

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Control Group ◽

Low Doses ◽

Chromosomal Damage ◽

Blood Lymphocytes ◽

Genome Damage ◽

Occupationally Exposed ◽

Index Value ◽

And Control

In the present study, we evaluated the induced genome damage in peripheral blood lymphocytes from a sample of nurses occupationally exposed to low doses of different chemicals. A comprehensive multi-biomarker approach using cytogenetic endpoints was employed for analyzing chromosomal aberrations (CAs) and sister chromatid exchange (SCE) assay. The study included 20 nurses and 20 control subjects matched in age, gender and smoking habits. Nurses were exposed to different chemicals, such as cytostatic drugs, anaesthetics, formaldehyde and other sterilizing gases. Significant differences were found between exposure group and control group in terms of SCEs frequency ( p < 0.001) but not in terms of replication index value ( p = 0.845) and CAs ( p = 0.236). Regression analyses indicated that the age and the exposure years did not influence the amount of the chromosomal damage among nurses. Vice versa, among controls, a positive correlation was found between the number of SCEs and age. In conclusion, our results suggest that a continuous long-term exposure to low doses of chemicals could result in increased levels of SCEs among nurses. This data emphasize the importance of biomonitoring nurses and other hospital workers handling drugs.

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