Androgen Biosynthesis in Leydig Cells after Testicular Desensitization by Luteinizing Hormone-Releasing ormone and Human Chorionic Gonadotropin

Endocrinology ◽  
1979 ◽  
Vol 105 (6) ◽  
pp. 1314-1321 ◽  
Author(s):  
M. L. DUFAU ◽  
S. CIGORRAGA
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Leydig Cells

scholarly journals Immunocytochemical localization of receptor human chorionic gonadotropin complexes in rat leydig cells.

1981 ◽  
Vol 29 (7) ◽  
pp. 813-816 ◽  
Author(s):  
H Rajaniemi ◽  
M Karjalainen ◽  
M Veijola ◽  
S Ritanen-Kaivamo ◽  
S Kellokumpu ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Cell Surface ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Leydig Cells ◽  
Intravenous Dose ◽  
Complex Method ◽  
Immunocytochemical Localization ◽  
Single Intravenous Dose ◽  
Surface Areas

Localization of receptor-bound human chorionic gonadotropin (hCG) in rat testis was studied by the peroxidase-antiperoxidase (PAP) complex method. The rats were injected with a single intravenous dose (1000 IU) of hCG. Three, 6, 12, and 24 hr after injection the testes were removed for localization of the hormone. The hormone localized to the periphery of the Leydig cells at all observation points. The intensity of the staining varied between the cells, suggesting that the number of receptors or the accessibility of the receptors to the circulating hormone varies from one cell to another. The staining surrounded the Leydig cells unevenly, but no progressive patching or capping was found. This observation suggests that hCG binds preferentially to the cell surface areas directed toward the capillaries. Compatible results were obtained with anti-hCG serum and with antisera against the hCG subunits. These results are consistent with previous observations that the luteinizing hormone (hCG) receptors accessible to the circulating hormone are located at the surface of the Leydig cells.

scholarly journals Immunocytochemical visualization of luteinizing hormone-human chorionic gonadotropin (LH-hCG) receptors in Leydig cells in primary culture.

1983 ◽  
Vol 31 (7) ◽  
pp. 898-904 ◽  
Author(s):  
M Begeot ◽  
C Mombrial ◽  
P M Dubois ◽  
M P Dubois ◽  
A Dazord ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Cell Surface ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Leydig Cells ◽  
Defined Medium ◽  
Short Exposure ◽  
Receptor Complexes ◽  
Immunocytochemical Reaction

Visualization of human chorionic gonadotropin (hCG) binding sites was obtained by immunocytochemical reaction on Leydig cells cultured in chemically defined medium. After a short in vitro incubation with hCG or luteinizing hormone (LH), the cells were fixed and the bound molecules were revealed using anti-hCG or anti-LH antisera. In both cases the immunocytochemical reaction appeared as granulations at the cell surface. After prolonged (48 hr) culture in the presence of 0.5, 5, or 50 ng/ml of hCG, the hormone receptor complex is still visible. Similarly, following short exposure to hCG (0.5 or 50 ng/ml) and one or two days of culture without hCG, the immunocytochemical reaction is still present. These observations suggest that the half-life of the bound hormone is very long. This in vitro system in which the amount and time of hormone exposure are precisely defined provides arguments in favor of a long-term maintenance of the receptor complexes at the cell surface.

Isolation of rat Leydig cells and precursor forms after administration of ethane dimethane sulfonate

1994 ◽  
Vol 266 (6) ◽  
pp. E975-E979 ◽  
Author(s):  
G. P. Risbridger ◽  
A. Davies
Keyword(s):  
Luteinizing Hormone ◽  
Cell Population ◽  
Chorionic Gonadotropin ◽  
Leydig Cell ◽  
Human Chorionic Gonadotropin ◽  
Leydig Cells ◽  
Steroid Production ◽  
Microscopic Appearance ◽  
Rat Testis ◽  
Adult Rat

The cytotoxic drug ethane dimethane sulfonate (EDS) has been extensively used as a means of studying the regeneration of Leydig cells in the adult rat testis. This study used the EDS-treated rat testis as a source of material for the isolation of regenerating Leydig cells and their precursors and describes the procedures required for the isolation of these cell preparations. As early as 13-15 days after EDS, cells in the precursor fraction can bind low, but detectable, levels of iodinated purified human chorionic gonadotropin. However, no luteinizing hormone (LH) response was detected in terms of steroid production. The precursor fraction of cells isolated from the EDS-treated rat testis 17-19 days after the administration of EDS was heterogeneous in light-microscopic appearance, but identifiable Leydig-like cells were present. The cells in this fraction were the first to exhibit the ability to respond to LH with the production of detectable levels of the reduced androgen, 5 alpha-androstane-3 alpha,17 beta-diol. The amount of androgen produced by both the Leydig cell and precursor fractions had increased by 21 days after EDS and reached the levels produced by immature adultlike Leydig cells, which can be isolated from the 20-day-old rat testes. These studies demonstrate that steroidogenically responsive precursor forms of Leydig cells can be isolated from the EDS-treated testes 17-19 days after depletion of the adult Leydig cell population.

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