1,25-Dihydroxyvitamin D3 Induces the Synthesis of Vitamin D-Dependent Calcium-Binding Protein in Cultured Chick Kidney Cells*

Endocrinology ◽  
1987 ◽  
Vol 120 (3) ◽  
pp. 894-902 ◽  
Author(s):  
GALE L. CRAVISO ◽  
KARLA P. GARRETT ◽  
THOMAS L. CLEMENS
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Kidney Cells ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Chick Kidney

Intestinal vitamin D-induced calcium-binding protein: time-course of immunocytological localization following 1,25-dihydroxyvitamin D3.

1983 ◽  
Vol 31 (3) ◽  
pp. 426-432 ◽  
Author(s):  
A N Taylor
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Calcium Transport ◽  
Time Course ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
Migration Rates ◽  
1,25 Dihydroxyvitamin D3 ◽  
Free Cells

The vitamin D-induced calcium-binding protein (CaBP) was localized in histological sections of chick duodenum using the peroxidase-antiperoxidase immunocytochemical technique. The time-course of appearance of CaBP in rachitic chicks was investigated from 0 to 120 hr after stimulation by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). CaBP was not routinely detected at 0 hr after 1,25(OH)2D3 administration. CaBP was first noted in some, but not all, of the samples taken 2 hr following 1,25(OH)2D3 and was detected in all 2 1/2 hr samples. The number of CaBP-containing absorptive cells and the apparent CaBP concentration both increased to a maximum at about 16-24 hr. At later times, as CaBP free cells migrated up the villi, the CaBP-containing cells decreased in number, but even at 120 hr post 1,25(OH)2D3 dose there were significant numbers of CaBP-containing cells present. The relationships between time-course of CaBP location on intestinal villi, enterocyte migration rates, and the time-course of 1,25(OH)2D3 stimulated intestinal calcium transport are discussed.

Vitamin D--dependent calcium binding protein: immunocytochemical localization in chick kidney

Science ◽  
1981 ◽  
Vol 214 (4517) ◽  
pp. 197-200 ◽  
Author(s):  
J Roth ◽  
B Thorens ◽  
W Hunziker ◽  
A. Norman ◽  
L Orci
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Immunocytochemical Localization ◽  
Chick Kidney
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