Differential effects of 1,25-dihydroxyvitamin D3 upon intestinal vitamin D3-dependent calbindin (a 28,000-dalton calcium binding protein) and its mRNA in D-replete and D-deficient chickens

1988 ◽  
Vol 260 (2) ◽  
pp. 645-652 ◽  
Author(s):  
Alan K. Hall ◽  
Helmut Reichel ◽  
Anthony W. Norman
Keyword(s):  
Vitamin D3 ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
Differential Effects ◽  
1,25 Dihydroxyvitamin D3

Rat duodenal calcium-binding protein messenger rna: Induction by 1,25-dihydroxyvitamin D3

1983 ◽  
Vol 19 (5) ◽  
pp. 1577-1582 ◽  
Author(s):  
Claude Desplan ◽  
Arlette Brehier ◽  
Christine Perret ◽  
Monique Thomassett
Keyword(s):  
Calcium Binding ◽  
Messenger Rna ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

Intestinal vitamin D-induced calcium-binding protein: time-course of immunocytological localization following 1,25-dihydroxyvitamin D3.

1983 ◽  
Vol 31 (3) ◽  
pp. 426-432 ◽  
Author(s):  
A N Taylor
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Calcium Transport ◽  
Time Course ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
Migration Rates ◽  
1,25 Dihydroxyvitamin D3 ◽  
Free Cells

The vitamin D-induced calcium-binding protein (CaBP) was localized in histological sections of chick duodenum using the peroxidase-antiperoxidase immunocytochemical technique. The time-course of appearance of CaBP in rachitic chicks was investigated from 0 to 120 hr after stimulation by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). CaBP was not routinely detected at 0 hr after 1,25(OH)2D3 administration. CaBP was first noted in some, but not all, of the samples taken 2 hr following 1,25(OH)2D3 and was detected in all 2 1/2 hr samples. The number of CaBP-containing absorptive cells and the apparent CaBP concentration both increased to a maximum at about 16-24 hr. At later times, as CaBP free cells migrated up the villi, the CaBP-containing cells decreased in number, but even at 120 hr post 1,25(OH)2D3 dose there were significant numbers of CaBP-containing cells present. The relationships between time-course of CaBP location on intestinal villi, enterocyte migration rates, and the time-course of 1,25(OH)2D3 stimulated intestinal calcium transport are discussed.

scholarly journals 1,25-Dihydroxyvitamin D3-induced calcium-binding protein: localization in organ-cultured embryonic chick duodenum.

1985 ◽  
Vol 33 (5) ◽  
pp. 477-479 ◽  
Author(s):  
R A Corradino ◽  
A N Taylor
Keyword(s):  
Organ Culture ◽  
Calcium Binding ◽  
De Novo ◽  
Binding Protein ◽  
Protein Localization ◽  
Calcium Binding Protein ◽  
Embryonic Chick ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces de novo biosynthesis of a specific calcium-binding protein (CaBP) in embryonic chick duodenum in organ culture. Using a highly sensitive and specific, peroxidase-antiperoxidase immunocytochemical procedure, 1,25(OH)2D3-induced CaBP in the organ-cultured duodenum was found only in the cytoplasm of absorptive cells, corresponding to its localization in rachitic chick duodenal cells after a single injection of 1,25(OH)2D3 in vivo. This observation, along with evidence correlating CaBP with calcium transport, strongly supports the use of the embryonic chick duodenal organ culture system as a physiologically relevant model of the vitamin D-dependent calcium absorptive mechanism.

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