scholarly journals Physiological Androgen Insensitivity of the Fetal, Neonatal, and Early Infantile Testis Is Explained by the Ontogeny of the Androgen Receptor Expression in Sertoli Cells

2008 ◽  
Vol 93 (11) ◽  
pp. 4408-4412 ◽  
Author(s):  
Héctor E. Chemes ◽  
Rodolfo A. Rey ◽  
Manuel Nistal ◽  
Javier Regadera ◽  
Mariana Musse ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Androgen Insensitivity

Ontogeny of anti-Mullerian hormone, 3β-hydroxysteroid dehydrogenase and androgen receptor expression during ovine fetal gonadal development

1997 ◽  
Vol 153 (1) ◽  
pp. 27-32 ◽  
Author(s):  
T Sweeney ◽  
P T K Saunders ◽  
M R Millar ◽  
A N Brooks
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Gonadal Development ◽  
Rete Testis ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Surrounding Tissue ◽  
Ovine Fetal

Abstract Anti-Mullerian hormone (AMH) and androgenic steroids are key factors regulating the masculinisation of the internal and external genitalia during fetal development. AMH is produced in Sertoli cells and causes regression of the Mullerian ducts in the male. 3β-Hydroxysteroid dehydrogenase (3β-HSD) is one of the key steroidogenic enzymes regulating testosterone production in Leydig cells. The objective of this experiment was to elucidate the development of the ovine fetal testes by identifying the spatio-temporal expression of AMH, 3β-HSD and androgen receptor expression within them. Fetuses from days 30 and 40 of gestation were fixed intact, while the gonads were dissected from the fetuses on days 70, 100 and 130 of gestation. Tissue was fixed in Bouin's fixative for 6 h, processed into paraffin wax and sections immunostained using rabbit anti-human AMH, 3β-HSD or androgen receptor antibodies. While seminiferous cords were absent on day 30 of gestation, pre-cord organisation was apparent and the gonad could be clearly distinguished from surrounding tissue by the presence of AMH and 3β-HSD immunopositive cells. Androgen receptor expression was not apparent at this stage. By day 40 of gestation the testis was organised into distinct seminiferous cords and intense immunostaining for AMH and 3β-HSD was present in Sertoli cells within the cords and Leydig cells in the interstitium respectively. Androgen receptor immunopositive cells were present in the interstitium but cells destined to develop into rete testis were immunonegative. By day 70 of gestation, the rete testis was organised in the centre of the testis and was strongly androgen receptor immunopositive. AMH and 3β-HSD expression was present in Sertoli and Leydig cells respectively. The expression of AMH, 3β-HSD and androgen receptor in the 100 and 130 day gestation fetuses was similar to that identified in the 70 day fetuses. In conclusion, Sertoli and Leydig precursor cells are present in the gonad prior to seminiferous cord formation and contain AMH and 3β-HSD at all stages of gestation examined. While androgen receptor immunoexpression was present in nuclei of interstitial cells from day 40 of gestation and in the rete testis from day 70 of gestation, Sertoli cells were immunonegative for androgen receptor at all of the stages examined. Journal of Endocrinology (1997) 153, 27–32

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 23 (5) ◽  
pp. 735-735
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

NF-κB and TNF-α stimulate androgen receptor expression in Sertoli cells

2003 ◽  
Vol 201 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
Frank J. Delfino ◽  
Jared N. Boustead ◽  
Charity Fix ◽  
William H. Walker
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Tnf Α

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells Accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 30 (3) ◽  
pp. 289-289
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells Accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 94 (5) ◽  
pp. 1818-1825 ◽  
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

scholarly journals Ethanol Extract of Young Papaya Seeds (Carica Papaya, L ) Lower the Level of testosterone, Spermatozoa Count and Expression of Androgen Receptors in Sertoli Cells of Adult Mice (Mus Musculus)

2021 ◽  
Vol 14 (1) ◽  
pp. 207-213
Author(s):  
I Dewa Made Ruspawan ◽  
I Made Bakta ◽  
I Nyoman Mangku Karmaya ◽  
Bagus Komang Satriyasa ◽  
I Gusti Kamasan Nyoman Arijana
Keyword(s):  
Body Weight ◽  
Androgen Receptor ◽  
Treatment Group ◽  
Sertoli Cells ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Control Group ◽  
Papaya Seeds

Background: Ethanol extract of young papaya seeds (Carica papaya L.) contains steroids that thought to has the antifertility property. This study aims to prove this hypothesis by measuring the low levels of testosterone hormone, spermatozoa count, and androgen receptor expression in sertoli cells. Material and Method:We use the randomized post-test only control group design on thirty-six male Balb/c strain adult male mice aged 12-14 weeks, weight 20-25 grams. They were randomly divided into a control and treatment group. The treatment group was given the same food as the control group plus ethanol extract of young papaya seeds 20 mg/20 gram body weight as much as 0.5 ml, orally every day for thirty-six days. Data were analyzed using the independent-sample T-test and Mann Whitney test. Result:The levels in the treatment group were significantly (p<0.05) lower than those in the control. Testosterone levels 31,64±1,91 vs 48,67±1,81 nmol/L, spermatozoa count 42,72±3,33 vs 75,89±4,71 cell/field of view, and androgen receptor expression 28,11±3.06% vs 55.07±2.49%. Conclusion:The ethanol extract of young papaya seeds 20 mg/20 g body weight has the antifertility property.

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