scholarly journals Ethanol Extract of Young Papaya Seeds (Carica Papaya, L ) Lower the Level of testosterone, Spermatozoa Count and Expression of Androgen Receptors in Sertoli Cells of Adult Mice (Mus Musculus)

2021 ◽  
Vol 14 (1) ◽  
pp. 207-213
Author(s):  
I Dewa Made Ruspawan ◽  
I Made Bakta ◽  
I Nyoman Mangku Karmaya ◽  
Bagus Komang Satriyasa ◽  
I Gusti Kamasan Nyoman Arijana
Keyword(s):  
Body Weight ◽  
Androgen Receptor ◽  
Treatment Group ◽  
Sertoli Cells ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Control Group ◽  
Papaya Seeds

Background: Ethanol extract of young papaya seeds (Carica papaya L.) contains steroids that thought to has the antifertility property. This study aims to prove this hypothesis by measuring the low levels of testosterone hormone, spermatozoa count, and androgen receptor expression in sertoli cells. Material and Method:We use the randomized post-test only control group design on thirty-six male Balb/c strain adult male mice aged 12-14 weeks, weight 20-25 grams. They were randomly divided into a control and treatment group. The treatment group was given the same food as the control group plus ethanol extract of young papaya seeds 20 mg/20 gram body weight as much as 0.5 ml, orally every day for thirty-six days. Data were analyzed using the independent-sample T-test and Mann Whitney test. Result:The levels in the treatment group were significantly (p<0.05) lower than those in the control. Testosterone levels 31,64±1,91 vs 48,67±1,81 nmol/L, spermatozoa count 42,72±3,33 vs 75,89±4,71 cell/field of view, and androgen receptor expression 28,11±3.06% vs 55.07±2.49%. Conclusion:The ethanol extract of young papaya seeds 20 mg/20 g body weight has the antifertility property.

scholarly journals Hepatoprotective Effect of Papaya Seed Ethanol Extract on Rifampicin Isoniazid-induced Rats

2021 ◽  
pp. 78-83
Author(s):  
Yessi Sunari Wahfar ◽  
I. Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Treatment Group ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Negative Group ◽  
Group I ◽  
Papaya Seeds ◽  
Papaya Seed

Aims: Hepatotoxicity induced by anti-tuberculosis drugs, including rifampicin and isoniazid (AT- DILI, Anti Tuberculosis-Drug Induced Liver Injury), is an adverse reaction followed by significant morbidity. Several in vivo and in vitro research has confirmed that papaya seeds contain various non-essentials, minerals, and fiber. Carica papaya role in disease prevention through modulation of various processes, such as anti-inflammatory, anti-diabetes, immunomodulatory activity, and antioxidant activity, suggests a role in neutralizing free radical generation and ultimately preventing pathogenesis. This study aimed to determine the hepatoprotective effect of ethanol extract of papaya seeds on rifampicin and isoniazid- induced rats. Study Design:  This study is experimental study. Methodology: The experimental animals in this study were divided into eight groups, including normal group, negative group 1, negative group 2, negative group 3, positive group, treatment group I (papaya seed ethanol extract dose of 100 mg/kgBW), treatment group II (papaya seed ethanol extract dose of 300 mg/kgBW), and treatment group III (papaya seed ethanol extract dose of 500 mg/kgBW), then the rats were dissected, and blood was taken for AST, ALT, ALP, GGT, and Bilirubin level measurements. Results: The results showed that papaya seed ethanol extract could reduce ALT, AST, ALP, GGT, and Bilirubin levels that were significantly different (P <0.05) than those in the negative control group. Conclusion: Flavonoid contains in the extract ethanol carica papaya has vita role to prevent the liver toxicity caused by isoniazid and rifampicin.

Ontogeny of anti-Mullerian hormone, 3β-hydroxysteroid dehydrogenase and androgen receptor expression during ovine fetal gonadal development

1997 ◽  
Vol 153 (1) ◽  
pp. 27-32 ◽  
Author(s):  
T Sweeney ◽  
P T K Saunders ◽  
M R Millar ◽  
A N Brooks
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Gonadal Development ◽  
Rete Testis ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Surrounding Tissue ◽  
Ovine Fetal

Abstract Anti-Mullerian hormone (AMH) and androgenic steroids are key factors regulating the masculinisation of the internal and external genitalia during fetal development. AMH is produced in Sertoli cells and causes regression of the Mullerian ducts in the male. 3β-Hydroxysteroid dehydrogenase (3β-HSD) is one of the key steroidogenic enzymes regulating testosterone production in Leydig cells. The objective of this experiment was to elucidate the development of the ovine fetal testes by identifying the spatio-temporal expression of AMH, 3β-HSD and androgen receptor expression within them. Fetuses from days 30 and 40 of gestation were fixed intact, while the gonads were dissected from the fetuses on days 70, 100 and 130 of gestation. Tissue was fixed in Bouin's fixative for 6 h, processed into paraffin wax and sections immunostained using rabbit anti-human AMH, 3β-HSD or androgen receptor antibodies. While seminiferous cords were absent on day 30 of gestation, pre-cord organisation was apparent and the gonad could be clearly distinguished from surrounding tissue by the presence of AMH and 3β-HSD immunopositive cells. Androgen receptor expression was not apparent at this stage. By day 40 of gestation the testis was organised into distinct seminiferous cords and intense immunostaining for AMH and 3β-HSD was present in Sertoli cells within the cords and Leydig cells in the interstitium respectively. Androgen receptor immunopositive cells were present in the interstitium but cells destined to develop into rete testis were immunonegative. By day 70 of gestation, the rete testis was organised in the centre of the testis and was strongly androgen receptor immunopositive. AMH and 3β-HSD expression was present in Sertoli and Leydig cells respectively. The expression of AMH, 3β-HSD and androgen receptor in the 100 and 130 day gestation fetuses was similar to that identified in the 70 day fetuses. In conclusion, Sertoli and Leydig precursor cells are present in the gonad prior to seminiferous cord formation and contain AMH and 3β-HSD at all stages of gestation examined. While androgen receptor immunoexpression was present in nuclei of interstitial cells from day 40 of gestation and in the rete testis from day 70 of gestation, Sertoli cells were immunonegative for androgen receptor at all of the stages examined. Journal of Endocrinology (1997) 153, 27–32

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 23 (5) ◽  
pp. 735-735
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

NF-κB and TNF-α stimulate androgen receptor expression in Sertoli cells

2003 ◽  
Vol 201 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
Frank J. Delfino ◽  
Jared N. Boustead ◽  
Charity Fix ◽  
William H. Walker
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Tnf Α

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells Accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 30 (3) ◽  
pp. 289-289
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

scholarly journals Lack of Androgen Receptor Expression in Sertoli Cells Accounts for the Absence of Anti-Mullerian Hormone Repression during Early Human Testis Development

2009 ◽  
Vol 94 (5) ◽  
pp. 1818-1825 ◽  
Author(s):  
Kahina Boukari ◽  
Geri Meduri ◽  
Sylvie Brailly-Tabard ◽  
Jean Guibourdenche ◽  
Maria Luisa Ciampi ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Human Testis ◽  
Testis Development ◽  
Early Human

scholarly journals MORTALITAS DAN RESORPSI FETUS MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK ETANOL TANAMAN SURUHAN (Peperomia pellucida (L.,) Kunth.)

2021 ◽  
pp. 194-202
Author(s):  
Hendri Busman ◽  
Nuning Nurcahyani ◽  
Yosi Dwi Saputra ◽  
Salman Farisi ◽  
Qotrunnada Salsabila
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Medicinal Use ◽  
One Way Anova ◽  
Different Doses

Tanaman Suruhan (Peperomia pellucida (L.,) Kunth.) merupakan tanaman obat yang memiliki senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, dan steroid. Berbagai senyawa kimia tersebut yang berpotensi sebagai obat tetapi perlu diperhatikan kemungkinan adanya efek samping terhadap organisme khususnya pada masa kehamilan. Penelitian ini bertujuan menguji efek teratogenik ekstrak tanaman suruhan terhadap fetus mencit (Mus musculus L.), meliputi mortalitas dan resorpsi. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan  20 ekor mencit betina dibagi ke dalam 4 kelompok yaitu: K(+) (Aquabides), P1 diberi ekstrak tanaman suruhan dengan dosis (1,68 mg/g bb), P2 (3,36 mg/g bb), dan P3 (6,72 mg/g bb). Hasil penelitian terhadap persentase fetus yang mengalami mortalitas dan resorpsi fetus antara kontrol K(+) dan perlakuan dengan ekstrak etanol suruhan (P1, P2, dan P3) tidak menunjukkan perbedaan yang bermakna secara statistik berdasarkan uji ANOVA satu faktor (p value 0,418). Hasil penelitian yang didapat menunjukkan bahwa ekstrak etanol tanaman suruhan tidak menyebabkan mortalitas pada fetus mencit, namun menyebabkan resorpsi pada fetus mencit pada pemberian dosis sebesar 1,62 mg/g BB, 3,36 mg/g BB, dan 6,72 mg/g BB.   Pepper elder (Peperomia pellucida (L.,) Kunth.) is a medicinal plant that has secondary metabolites such as alkaloids, flavonoids, saponins, tannins, and steroids. The side effects for organisms of those chemical compounds, which are potentially beneficial for their medicinal use, still need to be considered especially in pregnancy. This study aims to determine the teratogenic effects of pepper elder extract on mortality and resorption of mice (Mus musculus L.) fetus. This study uses total random sampling design with 4 treatments consisting of 20 female mice divided into 4 groups: the control group (K(+)) which is given Aqua distillation and the treatment groups which are all given the pepper elder extract with different doses, where the first treatment group (P1) is given 1.68 mg/g body weight of the extract, the second treatment group (P2) is given 3.36 mg/g body weight of the extract, and the third treatment group (P3) is given 6.72 mg/g body weight of the extract. The results obtained of mortality and resorption percentage of mice fetus between the control group (K(+)) and the treatment groups (P1, P2, and P3) showed that there is no statistically meaningful difference based on one-way ANOVA test (p value 0,418). This study showed that ethanol extract of pepper elder causes no mortality in mice fetus, yet it causes resorption on mice fetus at given doses of 1,62 mg/g body weight, 3,36 mg/g body weight, dan 6,72 mg/g body weight.

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