Matrix metalloproteinase production by cultured human endometrial stromal cells: identification of interstitial collagenase, gelatinase-A, gelatinase-B, and stromelysin-1 and their differential regulation by interleukin-1 alpha and tumor necrosis factor-alpha.

1994 ◽  
Vol 79 (2) ◽  
pp. 530-536 ◽  
Author(s):  
T J Rawdanowicz ◽  
A L Hampton ◽  
H Nagase ◽  
D E Woolley ◽  
L A Salamonsen
Keyword(s):  
Stromal Cells ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Differential Regulation ◽  
Gelatinase A ◽  
Endometrial Stromal Cells ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Interstitial Collagenase ◽  
Necrosis Factor

scholarly journals Recombinant tumor necrosis factor alpha and interleukin 1 alpha increase expression of c-abl protooncogene mRNA in cultured human marrow stromal cells

1989 ◽  
Vol 86 (17) ◽  
pp. 6788-6792 ◽  
Author(s):  
D F Andrews ◽  
J J Nemunaitis ◽  
J W Singer
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Stromal Cells ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Marrow Stromal Cells ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Recombinant Tumor Necrosis Factor ◽  
Necrosis Factor

Analysis of protooncogene RNA expression in marrow stromal cells from long-term marrow culture demonstrated high levels of c-abl 5-, 6-, and 7-kilobase (kb) RNA transcripts. In experiments on three independently derived simian virus 40-transformed marrow stromal cell lines, the expression of these c-abl transcripts was further increased in response to recombinant tumor necrosis factor alpha (1000 units/ml) and interleukin 1 alpha (10 units/ml). Although lymphocyte-conditioned medium predominantly up-regulated the 5-kb transcript, interleukin 1 alpha primarily affected the 6-kb transcript. The up-regulation of the 5-kb c-abl message correlated with up-regulation of the granulocyte/macrophage colony-stimulating factor transcript and down-regulation of procollagen I transcripts in transformed cells. These data suggest that c-abl plays roles in the regulation of extracellular matrix expression and in the regulation of hematopoietic growth factors by stromal cells.

Sign in / Sign up

Export Citation Format

Share Document