scholarly journals Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells

Development ◽  
2016 ◽  
Vol 143 (9) ◽  
pp. 1475-1481 ◽  
Author(s):  
Derek T. Peters ◽  
Christopher A. Henderson ◽  
Curtis R. Warren ◽  
Max Friesen ◽  
Fang Xia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Surface Marker ◽  
Asialoglycoprotein Receptor ◽  
Cell Surface Marker ◽  
Specific Cell ◽  
Specific Cell Surface

scholarly journals SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells

2011 ◽  
Vol 29 (11) ◽  
pp. 1011-1018 ◽  
Author(s):  
Nicole C Dubois ◽  
April M Craft ◽  
Parveen Sharma ◽  
David A Elliott ◽  
Edouard G Stanley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Surface Marker ◽  
Cell Surface Marker ◽  
Specific Cell ◽  
Specific Cell Surface

scholarly journals DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

2018 ◽  
Vol 11 (1) ◽  
pp. 115-127 ◽  
Author(s):  
Jongjin Park ◽  
Yeonsung Son ◽  
Na Geum Lee ◽  
Kyungmin Lee ◽  
Dong Gwang Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Surface Marker ◽  
Cell Surface Marker

scholarly journals Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17540 ◽  
Author(s):  
Shauna H. Yuan ◽  
Jody Martin ◽  
Jeanne Elia ◽  
Jessica Flippin ◽  
Rosanto I. Paramban ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Neural Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Surface Marker ◽  
Cell Surface Marker

scholarly journals Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

2014 ◽  
Vol 289 (27) ◽  
pp. 18846-18859 ◽  
Author(s):  
Angela Barone ◽  
Karin Säljö ◽  
John Benktander ◽  
Maria Blomqvist ◽  
Jan-Eric Månsson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Surface Marker ◽  
Cell Surface Marker

scholarly journals Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Kathryn F. Cogger ◽  
Ankit Sinha ◽  
Farida Sarangi ◽  
Emily C. McGaugh ◽  
Diane Saunders ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Marker ◽  
Cell Surface Marker ◽  
Specific Cell ◽  
Pancreatic Progenitors ◽  
Specific Cell Surface

scholarly journals The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 1212-1222 ◽  
Author(s):  
Nicholas Bredenkamp ◽  
Giuliano Giuseppe Stirparo ◽  
Jennifer Nichols ◽  
Austin Smith ◽  
Ge Guo
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Surface Marker ◽  
Cell Surface Marker

Abstract 65: Latrophilin-2 is a Specific Cell-surface Marker for Cardiac Progenitor Cells and Specifies Cardiac Lineage Commitment and Development

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Hyun-Jai Cho ◽  
Choon-Soo Lee ◽  
Jin-Woo Lee ◽  
Jung-Kyu Han ◽  
Han-Mo Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Development ◽  
Cardiac Differentiation ◽  
Specific Cell ◽  
Specific Marker ◽  
Cardiac Progenitor Cells ◽  
Cardiac Lineage

Backgrounds: The identification of a lineage-specific marker plays a pivotal role in understanding developmental process and is utilized to isolate a certain cell type with high purity for the therapeutic purpose. We here report a new cardiac-specific marker, and demonstrate its functional significance in the cardiac development. Methods and Results: When mouse pluripotent stem cells (ES and iPS cells) were stimulated with BMP4, Activin A, bFGF and VEGF, they differentiated into cardiac cells. To screen cell-surface expressing molecules on cardiac progenitor cells compared to undifferentiated mouse iPS and ES cells, we isolated Flk1+/PDGFRa+ cells at differentiation day 4 and performed microarray analysis. Among candidates, we identified a new G protein-coupled receptor, Latrophilin-2 (LPHN2) whose signaling pathway and its effect on cardiac differentiation is unknown. In sorting experiments under cardiac differentiation condition, LPHN2+ cells derived from pluripotent stem cells strongly expressed cardiac-related genes (Mesp1, Nkx2.5, aMHC and cTnT) and exclusively gave rise to beating cardiomyocytes, as compared with LPHN2- cells. LPHN2-/- mice revealed embryonically lethal and huge defects in cardiac development. Interestingly, LPHN2+/- heterozygotes were alive and fertile. For the purpose of cardiac regeneration, we transplanted iPS-derived LPHN2+ cells into the infarcted heart of adult mice. LPHN2+ cells differentiated into cardiomyocytes, and systolic function of left ventricle was improved and infarct size was reduced. We confirmed LPHN2 expression on human iPS and ES cell-derived cardiac progenitor cells and human heart. Conclusions: We demonstrate that LPHN2 is a functionally significant and cell-surface expressing marker for both mouse and human cardiac progenitor and cardiomyocytes. Our findings provide a valuable tool for isolating cardiac lineage cells from pluripotent stem cells and an insight into cardiac development and regeneration.

Sign in / Sign up

Export Citation Format

Share Document