The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation

1996 ◽  
Vol 109 (12) ◽  
pp. 2865-2874 ◽  
Author(s):  
H. Bastians ◽  
H. Ponstingl
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Shape Control ◽  
Cell Cycle Regulation ◽  
Budding Yeast ◽  
Mitotic Checkpoint ◽  
Human Protein ◽  
Predicted Amino Acid Sequence ◽  
Temperature Sensitive ◽  
Cycle Regulation

We identified a novel human protein serine/threonine phosphatase cDNA, designated protein phosphatase 6 (PP6) by using a homology-based polymerase chain reaction. The predicted amino acid sequence indicates a 35 kDa protein showing high homology to other protein phosphatases including human PP2A (57%), human PP4 (59%), rat PPV (98%), Drosophila PPV (74%), Schizosaccharomyces pombe ppe1 (68%) and Saccharomyces cerevisiae Sit4p (61%). In human cells, three forms of PP6 mRNA were found with highest levels of expression in testis, heart and skeletal muscle. The PP6 protein was detected in lysates of human heart muscle and in bull testis. Complementation studies using a temperature sensitive mutant strain of S. cerevisiae SIT4, which is required for the G1 to S transition of the cell cycle, showed that PP6 can rescue the mutant growth arrest. In addition, a loss of function mutant of S. pombe ppe1, described as a gene interacting with the pim1/spi1 mitotic checkpoint and involved in cell shape control, can be complemented by expression of human PP6. These data indicate that human PP6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, implying a function of PP6 in cell cycle regulation.

scholarly journals Identification of ricinoleic acid as an inhibitor of  Ca2+signal-mediated cell-cycle regulation in budding yeast

2010 ◽  
Vol 10 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Siriluck Attrapadung ◽  
Jun Yoshida ◽  
Ken-ichi Kimura ◽  
Masaki Mizunuma ◽  
Tokichi Miyakawa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Ricinoleic Acid ◽  
Cell Cycle Regulation ◽  
Budding Yeast ◽  
Cycle Regulation

scholarly journals Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

1998 ◽  
Vol 9 (12) ◽  
pp. 3273-3297 ◽  
Author(s):  
Paul T. Spellman ◽  
Gavin Sherlock ◽  
Michael Q. Zhang ◽  
Vishwanath R. Iyer ◽  
Kirk Anders ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Dna Microarrays ◽  
Full Description ◽  
Temperature Sensitive ◽  
Data Sets ◽  
Mrna Levels ◽  
Yeast Cultures ◽  
Yeast Genes ◽  
Cycle Regulation

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: α factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle–regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu

scholarly journals Cell-size regulation in budding yeast does not depend on linear accumulation of Whi5

2020 ◽  
Vol 117 (25) ◽  
pp. 14243-14250 ◽  
Author(s):  
Felix Barber ◽  
Ariel Amir ◽  
Andrew W. Murray
Keyword(s):  
Cell Cycle ◽  
Cell Size ◽  
Cell Cycle Regulation ◽  
Budding Yeast ◽  
Size Control ◽  
Size Distributions ◽  
Wild Type ◽  
Gal1 Promoter ◽  
The Mean ◽  
Cycle Regulation

Cells must couple cell-cycle progress to their growth rate to restrict the spread of cell sizes present throughout a population. Linear, rather than exponential, accumulation of Whi5, was proposed to provide this coordination by causing a higher Whi5 concentration in cells born at a smaller size. We tested this model using the inducibleGAL1promoter to make the Whi5 concentration independent of cell size. At an expression level that equalizes the mean cell size with that of wild-type cells, the size distributions of cells with galactose-induced Whi5 expression and wild-type cells are indistinguishable. Fluorescence microscopy confirms that the endogenous andGAL1promoters produce different relationships between Whi5 concentration and cell volume without diminishing size control in the G1 phase. We also expressed Cln3 from the GAL1 promoter, finding that the spread in cell sizes for an asynchronous population is unaffected by this perturbation. Our findings indicate that size control in budding yeast does not fundamentally originate from the linear accumulation of Whi5, contradicting a previous claim and demonstrating the need for further models of cell-cycle regulation to explain how cell size controls passage through Start.

scholarly journals Dynamin-Dependent Biogenesis, Cell Cycle Regulation and Mitochondrial Association of Peroxisomes in Fission Yeast

Traffic ◽  
2008 ◽  
Vol 9 (3) ◽  
pp. 353-365 ◽  
Author(s):  
Isabelle Jourdain ◽  
Dharani Sontam ◽  
Chad Johnson ◽  
Clément Dillies ◽  
Jeremy S. Hyams
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Cycle Regulation ◽  
Cycle Regulation

Fission yeast cyclin: subcellular localisation and cell cycle regulation

1989 ◽  
Vol 1989 (Supplement 12) ◽  
pp. 9-19 ◽  
Author(s):  
C. E. ALFA ◽  
R. BOOHER ◽  
D. BEACH ◽  
J. S. HYAMS
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Cycle Regulation ◽  
Subcellular Localisation ◽  
Cycle Regulation

scholarly journals Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network

2012 ◽  
Vol 8 (10) ◽  
pp. e1002732 ◽  
Author(s):  
Federico Vaggi ◽  
James Dodgson ◽  
Archana Bajpai ◽  
Anatole Chessel ◽  
Ferenc Jordán ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Polarity ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Cell Cycle Regulation ◽  
Interaction Network ◽  
Yeast Protein ◽  
Cycle Regulation ◽  
Yeast Protein Interaction

scholarly journals Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.

1991 ◽  
Vol 5 (2) ◽  
pp. 151-159 ◽  
Author(s):  
J Martinez ◽  
I Georgoff ◽  
J Martinez ◽  
A J Levine
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Cellular Localization ◽  
P53 Protein ◽  
Temperature Sensitive ◽  
Cycle Regulation

scholarly journals Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.

1993 ◽  
Vol 4 (3) ◽  
pp. 303-313 ◽  
Author(s):  
M Shimanuki ◽  
N Kinoshita ◽  
H Ohkura ◽  
T Yoshida ◽  
T Toda ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Shape Control ◽  
Budding Yeast ◽  
Suppressor Gene ◽  
Predicted Amino Acid Sequence ◽  
Permissive Temperature ◽  
Multicopy Suppressor ◽  
Isolation And Characterization ◽  
Cold Sensitive ◽  
Cell Shape Control

We isolated a fission yeast putative protein serine/threonine phosphatase gene designated ppe1+ by hybridization. The predicted amino acid sequence is similar to those of the fission yeast ppa2 (53% identity) and dis2 (39%) phosphatases, and highly similar to those of the budding yeast SIT4 (72%), Drosophila PPV (68%) and rabbit PPX (61%) phosphatases. Antibodies against ppe1 protein identified a 37-kd polypeptide in fission yeast. A gene disruption (designated delta ppe1) caused cold-sensitive lethality and short, pear-shaped cells. These phenotypes were fully suppressed by a plasmid carrying ppe1+. Three classes of multicopy suppressor genes for delta ppe1 were identified as follows: 1) ppa1+ and ppa2+ encoding type 2A-like phosphatases, 2) mitotically essential dis3+ similar to the budding yeast SSD1/SRK1, a suppressor for sit4, and 3) pck1+ coding for a protein kinase C-like kinase. Consistently, the budding yeast SIT4 gene was also a multicopy suppressor for delta ppe1. Phosphatase ppe1 may play a role in cell morphogenesis and mitosis by either regulating or being regulated by these multicopy suppressor gene products. Consistent with this hypothesis, double mutants ppe1-ppa2 and ppe1-pck1 are lethal at the permissive temperature.

Modeling nutrient effect on cell cycle regulation in fission yeast

2008 ◽  
Vol 136 ◽  
pp. S105
Author(s):  
Hu Teng ◽  
Xun Huang ◽  
Zhilong Xiu
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Cycle Regulation ◽  
Cycle Regulation
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