Studies on the Mechanism of Substrate Induction and L-Cyst(e)ine Repression of Alkaline Phosphatase in Mammalian Cell Cultures

1967 ◽  
Vol 2 (4) ◽  
pp. 545-555
Author(s):  
M. J. GRIFFIN ◽  
R. P. COX
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Cell Cultures ◽  
Phosphate Esters ◽  
Kidney Cell Line ◽  
Monkey Kidney Cell ◽  
African Green Monkey Kidney ◽  
Mammalian Cell Cultures ◽  
Green Monkey ◽  
Substrate Induction

The mechanisms of substrate induction and L-cyst(e)ine repression of alkaline phosphatase were studied in tissue culture using an established African green monkey kidney cell line (BS-C-I). L-Cyst(e)ine repression and substrate induction are mutually antagonistic. Evidence is presented which suggests that the increase in alkaline phosphatase levels induced by mono-phosphate esters may in part be due to protection of the enzyme from cellular degradation, while L-cyst(e)ine is believed to act either by repressing the synthesis of the enzyme or by selectively increasing its catabolism.

scholarly journals Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase

1967 ◽  
Vol 105 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Rody P. Cox ◽  
Paul Gilbert ◽  
Martin J. Griffin
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Inorganic Pyrophosphatase ◽  
Mammalian Cell Cultures ◽  
Single Protein ◽  
Pyrophosphatase Activity ◽  
Substrate Induction

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.

Detection of Hepatitis a Virus (HAV) in Drinking Water

1985 ◽  
Vol 17 (10) ◽  
pp. 23-38 ◽  
Author(s):  
M. D. Sobsey ◽  
S. E. Oglesbee ◽  
D. A. Wait ◽  
A. I. Cuenca
Keyword(s):  
Drinking Water ◽  
Cell Cultures ◽  
Kidney Cell ◽  
Hepatitis A ◽  
Tap Water ◽  
Enteric Viruses ◽  
Monkey Kidney ◽  
Monkey Kidney Cell ◽  
African Green Monkey Kidney ◽  
Green Monkey

Using recently developed cultivation and assay systems, currently available methods for concentrating enteric viruses from drinking water by adsorption to and subsequent elution from microporous filters followed by organic flocculation, were evaluated for their ability to recover HAV. Cell culture-adapted HAV (strain HMl75) in seeded tap water was efficiently adsorbed by both electronegative (Filterite) and electropositive (Virosorb lMDS) filters at pH and ionic conditions previously used for other enteric viruses. Adsorbed HAV was efficiently eluted from these filters by beef extract eluents at pH 9.5. Eluted HAV was further concentrated by acid precipitation (organic flocculation). Using optimum adsorption conditions for each type of filter, HAV was concentrated >100-fold from samples of seeded tap water with about 50% recovery of the initial infectious virus added to the samples. Electropositive filters were used to concentrate HAV from six samples of a fecally contaminated ground water supply implicated in an outbreak of hepatitis A in a rural community. HAV was detected and quantified in four of the six concentrate samples by radioimmunofocus assay in African green monkey kidney cell cultures. The presence of infectious HAV in water sample concentrates was further confirmed by experimental infection of chimpanzees and by virus isolation in African green monkey kidney cell cultures. The ability to recover and quantify HAV in contaminated drinking water with currently available methods should prove useful in further studies to determine the role of drinking water in HAV transmission.

scholarly journals VIRAL SUSCEPTIBILITY OF AN AFRICAN GREEN MONKEY KIDNEY CELL LINE-Vero

Uirusu ◽  
1968 ◽  
Vol 18 (3) ◽  
pp. 214-228 ◽  
Author(s):  
Heihachi ITOH ◽  
Yoshio MORIMOTO ◽  
Yutaka DOI ◽  
Tsuneo SANPE ◽  
Hiroshi TSUNODA
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cell Line ◽  
Monkey Kidney Cell ◽  
African Green Monkey Kidney ◽  
Viral Susceptibility ◽  
Green Monkey
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