Detection of small amounts of human DNA in human-rodent hybrids

1979 ◽  
Vol 38 (1) ◽  
pp. 391-403
Author(s):  
A. Rodgers
Keyword(s):  
Detection Limit ◽  
Hybrid Cell ◽  
Chinese Hamster ◽  
Filter Method ◽  
Somatic Cell Hybrids ◽  
Crna Probe ◽  
Reassociation Kinetics ◽  
Cell Hybrids ◽  
Human Dna ◽  
A Cell

A method of measuring semi-quantitatively small amounts of human DNA in irradiated human × mouse and irradiated human × Chinese-hamster somatic cell hybrids is described. One method uses molecular hybridization of cell DNA bound to nitrocellulose filters with a cRNA probe to Cot 0–1 human DNA. Alternatively hybrid cell DNA is reassociated in solution with a Cot 0–1 fraction of nick-translated human DNA. Formamide buffers give specificity to the reaction. The detection limit of the filter method is 0.2–0.5% equivalents and reassociation kinetics 0.005–0.1% equivalents of a human genome. Experiments with cell hybrids suggest that a fragment of repetitive DNA may be retained along with the selected genes after a cell fusion. In one case however, of a hybrid cell in which malignancy is suppressed, highly repetitious sequences were not found.

Transcription and translation of mitochondrial DNA in interspecific somatic cell hybrids

1986 ◽  
Vol 6 (6) ◽  
pp. 1951-1957
Author(s):  
F P Gillespie ◽  
T H Hong ◽  
J M Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Chinese Hamster ◽  
Mitochondrial Rna ◽  
Somatic Cell Hybrids ◽  
Rna Transcripts ◽  
Mitochondrial Rrna ◽  
Cell Hybrids

We examined the mitochondrial transcription and translation products of somatic cell hybrids constructed by the fusion of Chinese hamster and mouse cells. The hybrid cell lines OAC-k, OAC-l, and OAC-m contain approximately equal amounts of hamster and mouse mitochondrial DNA and produced mitochondrial rRNA from both parental species in the same ratio. Cell lines OAC-k, OAC-l, and OAC-m also produced poly(A)+ mouse mitochondrial RNA transcripts comparable in complexity and quantity to poly(A)+ RNA from the mouse parent. However, the overall level of poly(A)+ hamster mitochondrial RNA from these hybrids was significantly reduced compared with that from the hamster parent. The hybrid cells also lacked several poly(A)+ RNA species found in the hamster parent, but contained additional minor transcripts. The mitochondrially coded proteins of the OAC-k, OAC-l, and OAC-m cells were predominantly encoded by the mouse mitochondrial DNA.

scholarly journals Transcription and translation of mitochondrial DNA in interspecific somatic cell hybrids.

1986 ◽  
Vol 6 (6) ◽  
pp. 1951-1957 ◽  
Author(s):  
F P Gillespie ◽  
T H Hong ◽  
J M Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Chinese Hamster ◽  
Mitochondrial Rna ◽  
Somatic Cell Hybrids ◽  
Rna Transcripts ◽  
Mitochondrial Rrna ◽  
Cell Hybrids

We examined the mitochondrial transcription and translation products of somatic cell hybrids constructed by the fusion of Chinese hamster and mouse cells. The hybrid cell lines OAC-k, OAC-l, and OAC-m contain approximately equal amounts of hamster and mouse mitochondrial DNA and produced mitochondrial rRNA from both parental species in the same ratio. Cell lines OAC-k, OAC-l, and OAC-m also produced poly(A)+ mouse mitochondrial RNA transcripts comparable in complexity and quantity to poly(A)+ RNA from the mouse parent. However, the overall level of poly(A)+ hamster mitochondrial RNA from these hybrids was significantly reduced compared with that from the hamster parent. The hybrid cells also lacked several poly(A)+ RNA species found in the hamster parent, but contained additional minor transcripts. The mitochondrially coded proteins of the OAC-k, OAC-l, and OAC-m cells were predominantly encoded by the mouse mitochondrial DNA.

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

1973 ◽  
Vol 20 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Ans Jongsma ◽  
Harry Someren ◽  
Andries Westerveld ◽  
Ann Hagemeijer ◽  
Peter Pearson
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Retention of both parental mitochondrial DNA species in mouse-Chinese hamster somatic cell hybrids

1984 ◽  
Vol 10 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Steven H. Zuckerman ◽  
Joseph F. Solus ◽  
Frances P. Gillespie ◽  
Jerome M. Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Genetical Studies on the Multiple Forms of Human Guanylate Kinase in Man-Chinese Hamster Somatic Cell Hybrids

1974 ◽  
Vol 24 (5-6) ◽  
pp. 415-423 ◽  
Author(s):  
Meera Khan ◽  
W.R.T. Los ◽  
P.L. Pearson ◽  
A. Westerveld ◽  
D. Bootsma
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Multiple Forms ◽  
Guanylate Kinase ◽  
Cell Hybrids

5 Persistence of fragmented mouse HSR chromatin in mouse-chinese hamster somatic cell hybrids resistant to actinomycin D

1987 ◽  
Vol 28 (1) ◽  
pp. 29
Author(s):  
G. Levan ◽  
A.-H. Jakobsson ◽  
U. Arnason ◽  
A. Levan ◽  
T. Martinsson
Keyword(s):  
Somatic Cell ◽  
Actinomycin D ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids
Sign in / Sign up

Export Citation Format

Share Document