scholarly journals Cell cycle-dependent variations in RNA polymerase activity in the nuclear and cytoplasmic fractions of Physarum polycephalum.

1985 ◽  
Vol 10 (1) ◽  
pp. 55-62
Author(s):  
Fumio Sawada
Keyword(s):  
Cell Cycle ◽  
Rna Polymerase ◽  
Physarum Polycephalum ◽  
Polymerase Activity ◽  
Rna Polymerase Activity ◽  
Cycle Dependent

In vitro transcription of RNA in nuclei, nucleoli and chromatin from physarum polycephalum

1977 ◽  
Vol 26 (1) ◽  
pp. 267-279
Author(s):  
K.E. Davies ◽  
I.O. Walker
Keyword(s):  
Rna Polymerase ◽  
Physarum Polycephalum ◽  
Rna Synthesis ◽  
In Vitro Transcription ◽  
Polymerase Activity ◽  
Controlled Conditions ◽  
Rna Polymerase Activity ◽  
Alpha Amanitin

Methods for isolating nuclei, nucleoli and chromatin from Physarum polycephalum which retain high levels of endogenous RNA polymerase activity are described. Under carefully controlled conditions with respect to mono- and divalent cation concentrations RNA synthesis in nuclei displayed linear kinetics for at least 30 min and the RNA products had a similar size distribution to nuclear RNA synthesis observed in vivo. Chromatin showed 60% of the nuclear transcriptional activity but no conditions were found where faithful transcription of the template occurred. Isolated nucleoli were 5-fold more active than nuclei and the endogenous RNA polymerase activity was insensitive to alpha-amanitin. Under carefully controlled conditions, the nucleoli appeared to support the accurate transcription, re-initiation and processing of rRNA chains in vitro.

More evidence for replication-transcription-coupling in Physarum polycephalum

1980 ◽  
Vol 41 (1) ◽  
pp. 105-113
Author(s):  
G. Pierron ◽  
H.W. Sauer
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Physarum Polycephalum ◽  
Mitotic Cycle ◽  
Polymerase Activity ◽  
S Phase ◽  
Isolated Nuclei ◽  
Rna Polymerase Activity ◽  
High Level ◽  
Alpha Amanitin

Endogenous RNA polymerase activity of isolated nuclei from Physarum polycephalum was determined at high (400 mM KCl) and low (5–100 mM KCl) ionic strength. The activity of RNA polymerase B (alpha-amanitin-sensitive UMP incorporation) and of RNA polymerase A (plus C) (alpha-amanitin-resistant UMP incorporation) was compared in accurately sized nuclear samples derived from macroplasmodia at distinct points of the mitotic cycle. Minimum total RNA polymerase activity was detected in metaphase nuclei. A constant level of RNA polymerase B activity was detected at all other stages of the mitotic cycle, if nuclei were assayed at high ionic strength. However, a high level in S-phase, a low level in G2-phase and again a high level in early prophase were measured, if nuclei were assayed at low ionic strength. Inhibition of DNA synthesis by hydroxyurea in vivo had a selective and drastic effect on in vitro RNA polymerase activity of isolated nuclei derived from S-phase plasmodia, yielding up to 100% inhibition in early S-phase.

scholarly journals Cell cycle-dependent regulation of RNA polymerase II basal transcription activity

1995 ◽  
Vol 23 (20) ◽  
pp. 4050-4054 ◽  
Author(s):  
Masatomo Yonaha ◽  
Taku Chibazakura ◽  
Shigetaka Kitajima ◽  
Yukio Yasukochi
Keyword(s):  
Cell Cycle ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Basal Transcription ◽  
Transcription Activity ◽  
Cycle Dependent

scholarly journals Cell cycle dependent change in the endogenous phosphorylation of nucleolar proteins of Physarum polycephalum

FEBS Letters ◽  
1981 ◽  
Vol 124 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Toshiko Shibayama ◽  
Shouzou Sawai ◽  
Kazuyasu Nakaya ◽  
Yasuharu Nakamura
Keyword(s):  
Cell Cycle ◽  
Physarum Polycephalum ◽  
Nucleolar Proteins ◽  
Dependent Change ◽  
Cycle Dependent

Pharmaceutical interference of the EWS-FLI1-driven transcriptome by co-targeting H3K27ac and RNA polymerase activity in Ewing Sarcoma

2021 ◽  
pp. molcanther.MCT-20-0489-A.2020
Author(s):  
Daniel A. R. Heisey ◽  
Sheeba Jacob ◽  
Timonthy L Lochmann ◽  
Richard Kurupi ◽  
Maninderjit S. Ghotra ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Ewing Sarcoma ◽  
Polymerase Activity ◽  
Rna Polymerase Activity

RNA polymerase activity in virions from Ustilago maydis

1984 ◽  
Vol 4 (1) ◽  
pp. 188-194
Author(s):  
B S Ben-Tzvi ◽  
Y Koltin ◽  
M Mevarech ◽  
A Tamarkin
Keyword(s):  
Rna Polymerase ◽  
Viral Genome ◽  
Ustilago Maydis ◽  
Polymerase Activity ◽  
Reaction Products ◽  
Double Stranded Rna ◽  
Rna Molecules ◽  
Rna Transcripts ◽  
Rna Polymerase Activity

RNA polymerase activity is associated with the double-stranded RNA virions of Ustilago maydis. The reaction products of the polymerase activity are single-stranded RNA molecules. The RNA molecules synthesized are homologous to the three classes of double-stranded RNA molecules that typify the viral genome. The single-stranded RNA synthesized is released from the virions. The molecular weight of the single-stranded RNA transcripts is about half the size of the double-stranded RNA segments, and thus, it appears that in the in vitro reaction, full-length transcripts can be obtained.

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