scholarly journals Effect of insulin on murine megakaryocytopoiesis in a liquid culture system.

1987 ◽  
Vol 12 (3) ◽  
pp. 311-316 ◽  
Author(s):  
Yasuko Watanabe ◽  
Minako Kawada ◽  
Bonro Kobayashi
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System

scholarly journals A liquid culture system for murine megakaryocyte progenitor cells

Blood ◽  
1982 ◽  
Vol 59 (2) ◽  
pp. 250-257
Author(s):  
T Nagasawa ◽  
M Nakazawa ◽  
T Abe
Keyword(s):  
Progenitor Cells ◽  
Conditioned Medium ◽  
Culture System ◽  
Liquid Culture ◽  
Culture Medium ◽  
Pokeweed Mitogen ◽  
Plasma Clot ◽  
Liquid Culture System ◽  
Cell Conditioned Medium ◽  
Number Of Cells

A liquid culture system is described for murine megakaryocyte progenitor cells (CFU-M) in the presence of pokeweed-mitogen-stimulated spleen-cell conditioned medium. There were dose-related responses between the number of CFU-M developed and the number of cells cultured and the dosage of conditioned medium in this liquid culture system. Murine CFU-M were abundantly cloned in this system an the plating efficiency was similar in comparison with that in a plasma clot system. The acetylcholinesterase-positive colonies (more than 4 acetylcholinesterase-positive cells) were clearly seen on day 3 of culture, and they reached a maximum (60.5 +/- 10.7/2 x 10(5) cells) on day 7 of culture. Ultrastructural analyses of megakaryocytic maturation in this system showed that a few megakaryocytes produced platelets that were released in the culture medium on day 5 of culture. This liquid culture system is suitable for the study of the dynamic process of the megakaryocyte-platelet system.

scholarly journals Effect of gangliosides on murine megakaryocytopoiesis in a liquid culture system.

1988 ◽  
Vol 13 (4) ◽  
pp. 293-300
Author(s):  
Yasuko Watanabe ◽  
Masami Taniguchi ◽  
Nobuko Fukamachi ◽  
Bonro Kobayashi
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System

scholarly journals Liquid Culture System for Mature Pollen in Hyuganatsu (Citrus tamurana hort. ex Tanaka)

2012 ◽  
Vol 11 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Asuka Uchida ◽  
Akiha Abe ◽  
Yoichiro Hoshino ◽  
Hisato Kunitake
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Mature Pollen ◽  
Liquid Culture System

The development of a liquid culture system for the growth of human bone marrow

1980 ◽  
Vol 4 (5) ◽  
pp. 449-461 ◽  
Author(s):  
I.R.G. Toogood ◽  
T.M. Dexter ◽  
T.D. Allen ◽  
T. Suda ◽  
L.G. Lajtha
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Liquid Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Liquid Culture System
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