The development of a liquid culture system for the growth of human bone marrow

1980 ◽  
Vol 4 (5) ◽  
pp. 449-461 ◽  
Author(s):  
I.R.G. Toogood ◽  
T.M. Dexter ◽  
T.D. Allen ◽  
T. Suda ◽  
L.G. Lajtha
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Liquid Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Liquid Culture System

A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system

2006 ◽  
Vol 34 (10) ◽  
pp. 1353-1359 ◽  
Author(s):  
Raquel Gonçalves ◽  
Cláudia Lobato da Silva ◽  
Joaquim M.S. Cabral ◽  
Esmail D. Zanjani ◽  
Graça Almeida-Porada
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Feeder Layer ◽  
Hematopoietic Stem ◽  
Serum Free

Toxicity of Intravenous Catheters Tested in a Human Bone Marrow Culture System

2009 ◽  
Vol 202 (1-6) ◽  
pp. 103-106 ◽  
Author(s):  
B. Thing Mortensen ◽  
Lis E. Jensen ◽  
Seren Knudtzon ◽  
Nis I. Nissen
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Bone Marrow Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Intravenous Catheters

Characterization and phenotypic analysis of differentiating CD34+human bone marrow cells in liquid culture

2009 ◽  
Vol 59 (5) ◽  
pp. 318-326 ◽  
Author(s):  
Sherilyn Gross ◽  
Karen Helm ◽  
Jennifer J. Gruntmeir ◽  
Wayne S. Stillman ◽  
David W. Pyatt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Liquid Culture ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Phenotypic Analysis ◽  
Marrow Cells

scholarly journals Long-term human bone marrow cultures

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 118-124
Author(s):  
WG Hocking ◽  
DW Golde
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Murine Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures

Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4–9 wk. Morphologically recognizable granulopoiesis occurs for 4–6 wk and erythropoiesis for 1.5–2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adherent population is composed of macrophages, fibroblast-like cells, and flat pavement- like cells. Fat-containing cells are not prominant. This culture system provides a means to study human hematopoietic cell proliferation and differentiation in vitro, as well as to examine interactions between stromal cells and hematopoietic and lymphoid progenitors.

scholarly journals Correlation of Radioactive Hemin Formation with Morphologic Alterations in Cultures of Human Bone Marrow

Blood ◽  
1959 ◽  
Vol 14 (11) ◽  
pp. 1213-1222 ◽  
Author(s):  
EDWARD R. POWSNER ◽  
LAWRENCE BERMAN
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Culture System ◽  
In Vitro System ◽  
In Vitro Cell Culture ◽  
The Times

Abstract A method for measurement of radioactive hemin formation has been applied to the in vitro system for culture of human erythroblasts previously described. The results of these measurements have been shown to correlate with a morphologic parameter of maturation, the increase of orthochromic cells, in the same in vitro cell culture system. The significance of these findings as well as the differences in the times of formation of heme and the increase of orthochromic cells has been discussed. It is proposed that both the formation of radioactive hemin and the increase of orthochromic cells may be useful in the measurement of different aspects of the effects of various plasmas on the maturation of normal and abnormal human erythroblasts.

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