scholarly journals Extension of Long Cellular Processes of Hepatic Stellate Cells Cultured on Extracellular Type I Collagen Gel by Microtubule Assembly: Observation Utilizing Time-lapse Video-microscopy.

1997 ◽  
Vol 22 (4) ◽  
pp. 487-492 ◽  
Author(s):  
Mitsutaka Miura ◽  
Mitsuru Sato ◽  
Itaru Toyoshima ◽  
Haruki Senoo
Keyword(s):  
Hepatic Stellate Cells ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Stellate Cells ◽  
Time Lapse ◽  
Microtubule Assembly ◽  
Type I ◽  
Cellular Processes ◽  
Type I Collagen Gel ◽  
Time Lapse Video

scholarly journals EFFECT OF COLLAGEN I GEL ON APOPTOSIS OF RAT HEPATIC STELLATE CELLS

2013 ◽  
Vol 56 (2) ◽  
pp. 73-79
Author(s):  
Lenka Bittnerová ◽  
Alena Jiroutová ◽  
Emil Rudolf ◽  
Martina Řezáčová ◽  
Jiří Kanta
Keyword(s):  
Hepatic Stellate Cells ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Stellate Cells ◽  
Type I ◽  
Function Type ◽  
Fibrotic Liver ◽  
Normal Rat ◽  
And Function

Activated hepatic stellate cells (HSC) are a major source of fibrous proteins in cirrhotic liver. Inducing or accelerating their apoptosis is a potential way of liver fibrosis treatment. Extracellular matrix (ECM) surrounding cells in tissue affects their differentiation, migration, proliferation and function. Type I collagen is the main ECM component in fibrotic liver. We have examined how this protein modifies apoptosis of normal rat HSC induced by gliotoxin, cycloheximide and cytochalasin D in vitro and spontaneous apoptosis of HSC isolated from CCl4-damaged liver. We have found that type I collagen gel enhances HSC apoptosis regardless of the agent triggering this process.

Apical beta 1 integrin in polarized MDCK cells mediates tubulocyst formation in response to type I collagen overlay

1996 ◽  
Vol 109 (7) ◽  
pp. 1875-1889 ◽  
Author(s):  
A. Zuk ◽  
K.S. Matlin
Keyword(s):  
Plasma Membranes ◽  
Type I Collagen ◽  
Mdck Cells ◽  
Basolateral Membrane ◽  
Collagen Gel ◽  
Type I ◽  
Membrane Markers ◽  
Polarized Epithelia ◽  
Beta 1 Integrin ◽  
Type I Collagen Gel

A number of epithelia form tubulocysts in vitro when overlaid with type I collagen gel. Because collagen receptors are generally believed to be expressed on the basolateral domain, the mechanism by which collagen elicits this morphogenetic response from the apical surface is unclear. To investigate the role of beta 1 integrins, the major receptor family for collagen, in this process, we overlaid polarized monolayers of MDCK II cells grown on permeable supports with type I collagen gel and correlated integrin polarity with the polarity of other apical and basolateral membrane markers during tubulocyst formation. Polarized monolayers of one clone of MDCK II cells, referred to as Heidelberg MDCK, initially respond to collagen overlay by stratifying; within 48 hours, lumena develop between the cell layers giving rise to tubulocysts. Tight junctions remain intact during tubulocyst formation because transepithelial electrical resistance does not significantly change. Major alterations are observed, however, in the expression and localization of apical and basolateral membrane markers. beta 1 integrins are necessary for tubulocyst morphogenesis because a function-blocking antibody administered to the apical pole of the cells completely inhibits the formation of these structures. To determine how apical-cell collagen interactions elicit tubulocyst formation, we examined whether beta 1 integrins are mobilized to apical plasma membranes in response to collagen overlay. We found that in the absence of collagen, polarized monolayers of Heidelberg MDCK cells endogenously express on apical plasma membranes a small pool of the beta 1 family, including alpha 2 beta 1 and alpha 3 beta 1. Collagen overlay does not mobilize additional beta 1 integrins to apical domains. If beta 1 integrins are not already apically expressed, as in the C6 MDCK cell line (Schoenenberger et al. (1994) J. Cell Biol. 107, 527–541), beta 1 integrins are not directed apically and tubulocysts do not develop in response to collagen. Thus, interaction of beta 1 integrin pre-existing on apical plasma membranes of polarized epithelia with type I collagen gel is the mechanism by which apical application of collagen elicits the formation of tubulocysts. Depolarized integrins on apical plasma membranes of polarized epithelia may be relevant to the pathogenesis of disease and injury.

Coculture of Human Hair Follicles and Dermal Papillae in Type I Collagen Gel

2006 ◽  
Vol 642 (1) ◽  
pp. 454-456 ◽  
Author(s):  
SEIJI ARASE ◽  
YASUSHI SADAMOTO ◽  
SHOUJI KATOH
Keyword(s):  
Human Hair ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Hair Follicles ◽  
Type I ◽  
Dermal Papillae ◽  
Type I Collagen Gel

The Physicochemical Property of Shark Type I Collagen Gel and Membrane

2000 ◽  
Vol 48 (6) ◽  
pp. 2028-2032 ◽  
Author(s):  
Yoshihiro Nomura ◽  
Shinzi Toki ◽  
Yasuhiro Ishii ◽  
Kunio Shirai
Keyword(s):  
Physicochemical Property ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel

scholarly journals Reciprocal Modulation of Matrix Metalloproteinase-13 and Type I Collagen Genes in Rat Hepatic Stellate Cells

2003 ◽  
Vol 162 (6) ◽  
pp. 1771-1780 ◽  
Author(s):  
Benjamin Schaefer ◽  
Ana María Rivas-Estilla ◽  
Noemí Meraz-Cruz ◽  
Miguel Arturo Reyes-Romero ◽  
Zamira H. Hernández-Nazara ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Hepatic Stellate Cells ◽  
Type I Collagen ◽  
Stellate Cells ◽  
Type I ◽  
Matrix Metalloproteinase 13 ◽  
Collagen Genes

Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel

2008 ◽  
Vol 133 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Yuji Hirano ◽  
Naoki Ishiguro ◽  
Masahiro Sokabe ◽  
Masaharu Takigawa ◽  
Keiji Naruse
Keyword(s):  
Cell Line ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel

Bovine chondrocyte behaviour in three-dimensional type I collagen gel in terms of gel contraction, proliferation and gene expression

Biomaterials ◽  
2006 ◽  
Vol 27 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Laurent Galois ◽  
Sandrine Hutasse ◽  
Delphine Cortial ◽  
Cécile F. Rousseau ◽  
Laurent Grossin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel
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