scholarly journals Enzyme Labeling in Steroid Enzyme Immunoassays. The p-Nitrophenyl Ester Method for Alkaline Phosphatase and Glucose Oxidase Labelings.

1992 ◽  
Vol 40 (5) ◽  
pp. 1325-1326 ◽  
Author(s):  
Hiroshi HOSODA ◽  
Katsuyuki FUKUDA ◽  
Junichi GOTO
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Enzyme Immunoassays ◽  
Ester Method ◽  
Nitrophenyl Ester

Some Methodological Modifications of the Technicon "SMA 12/60 AutoAnalyzer" System

1972 ◽  
Vol 18 (7) ◽  
pp. 630-642 ◽  
Author(s):  
Tevfik K Bigat ◽  
Abraham Saifer
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Aspartate Aminotransferase ◽  
Inorganic Phosphate ◽  
New Method ◽  
Green Method ◽  
Bromcresol Green ◽  
Made In

Abstract Methodological modifications have been made in our "SMA 12/60 AutoAnalyzer" system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents used in the determination of protein, inorganic phosphate, and aspartate aminotransferase. A general approach that is useful for adapting a new method to a multichannel AutoAnalyzer system is discussed.

Utilizing ultrafiltration to remove alkaline phosphatase from clinical analyzer water

2006 ◽  
Vol 44 (5) ◽  
Author(s):  
Julien Bôle ◽  
Stéphane Mabic
Keyword(s):  
Alkaline Phosphatase ◽  
Water Samples ◽  
Bacterial Contamination ◽  
Pure Water ◽  
Experimental Conditions ◽  
Enzyme Immunoassays ◽  
Selection Of

AbstractAlkaline phosphatase (ALP) conjugated to antibodies is often used in enzyme immunoassays (EIAs). These assays are notably sensitive to experimental conditions. A possible source of interference is bacterial ALP, which is released when bacterial contamination occurs in clinical analyzers. Preliminary experiments led to the selection of a detection kit, ALP source, and specific types of tubes for collecting water samples and performing assays. The release of ALP from various strains of bacteria identified in pure water was demonstrated (10–30×10

Highly sensitive amperometric measurement of alkaline phosphatase activity with glucose oxidase amplification

1995 ◽  
Vol 382 (1-2) ◽  
pp. 129-135 ◽  
Author(s):  
Leopoldo Della Ciana ◽  
Giuliana Bernacca ◽  
Franco Bordin ◽  
Simona Fenu ◽  
Fulvio Garetto
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Highly Sensitive

The preparation of some sequential polypeptides by the p-Nitrophenyl ester method

1966 ◽  
Vol 19 (8) ◽  
pp. 1503 ◽  
Author(s):  
FHC Stewart
Keyword(s):  
Protecting Group ◽  
Active Ester ◽  
Ester Method ◽  
Nitrophenyl Ester

A number of sequential polypeptides have been obtained by polymerization of peptide p-nitrophenyl esters. The majority of the products contained γ-ethyl-L-glutamyl and S-benzyl-L-cysteinyl residues in various proportions. The active ester monomers were synthesized by the mixed carbonic anhydride procedure using p-nitrophenyl as a carboxyl-protecting group. Some of the limitations of this approach are discussed.

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