Some Methodological Modifications of the Technicon "SMA 12/60 AutoAnalyzer" System

1972 ◽  
Vol 18 (7) ◽  
pp. 630-642 ◽  
Author(s):  
Tevfik K Bigat ◽  
Abraham Saifer
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Aspartate Aminotransferase ◽  
Inorganic Phosphate ◽  
New Method ◽  
Green Method ◽  
Bromcresol Green ◽  
Made In

Abstract Methodological modifications have been made in our "SMA 12/60 AutoAnalyzer" system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents used in the determination of protein, inorganic phosphate, and aspartate aminotransferase. A general approach that is useful for adapting a new method to a multichannel AutoAnalyzer system is discussed.

Comparison of several methods for semiquantitative determination of urinary protein.

1977 ◽  
Vol 23 (5) ◽  
pp. 876-879 ◽  
Author(s):  
W L Gyure
Keyword(s):  
Serum Albumin ◽  
Salt Concentration ◽  
Urine Samples ◽  
Urine Protein ◽  
Sulfosalicylic Acid ◽  
Green Method ◽  
Semiquantitative Determination ◽  
Acid Method ◽  
Bromcresol Green

Abstract Two types of urine protein dipsticks and the sulfosalicylic acid method were compared for their accuracy and specificity, with use of urine samples supplemented with various proteins. Dipsticks yield accurate results when the protein under consideration is restricted to albumin; the sulfosalicylic acid method accurately determines many kinds of proteins in addition to albumin. Detergents affect each of the methods, but changes in salt concentration only affect results by dipstick procedures. Dipsticks, which are based on the protein-error principle for indicators, are subject to some of the conditions that apply to the bromcresol green method for serum albumin determination.

Improved continuous-flow (SMAC) determination of serum albumin.

1978 ◽  
Vol 24 (7) ◽  
pp. 1191-1193 ◽  
Author(s):  
G Cederblad ◽  
B E Hickey ◽  
A Hollender ◽  
G Akerlund
Keyword(s):  
Reaction Time ◽  
Serum Albumin ◽  
Acute Phase ◽  
Continuous Flow ◽  
Green Method ◽  
Acute Phase Reactants ◽  
Modified Method ◽  
Continuous Flow Method ◽  
Bromcresol Green

Abstract The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.

scholarly journals Enzyme Aggregation and Fragmentation Induced by Catalysis Relevant Species

2021 ◽  
Author(s):  
Kayla Gentile ◽  
Ashlesha Bhide ◽  
Joshua Kauffman ◽  
Subhadip Ghosh ◽  
Subhabrata Maiti ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Catalytic Activity ◽  
Glucose Oxidase ◽  
Inorganic Phosphate ◽  
Chemical Species ◽  
Low Ph ◽  
Native Structure ◽  
Native Form

It is usually assumed that enzymes retain their native structure during catalysis. However, the aggregation and fragmentation of proteins can be difficult to detect and sometimes conclusions are drawn based on the assumption that the protein is in its native form. We have examined three model enzymes, alkaline phosphatase (AkP), hexokinase (HK) and glucose oxidase (GOx). We find that these enzymes aggregate or fragment after addition of chemical species directly related to their catalysis. We used several independent techniques to study this behavior. Specifically, we found that glucose oxidase and hexokinase fragment in the presence of D-Glucose but not L-glucose, while hexokinase aggregates in the presence of Mg2+ ion and either ATP or ADP at low pH. Alkaline phosphatase aggregates in the presence of Zn2+ ion and inorganic phosphate. The aggregation of hexokinase and alkaline phosphatase does not appear to attenuate their catalytic activity. Our study indicates that specific multimeric structures of native enzymes may not be retained during catalysis and suggests pathways for different enzymes to associate or separate over the course of substrate turnover.

Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

1966 ◽  
Vol 12 (10) ◽  
pp. 701-708 ◽  
Author(s):  
J H Wilkinson ◽  
Ann V Vodden
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Concentration ◽  
Inorganic Phosphate ◽  
Clinical Laboratory ◽  
Serum Alkaline Phosphatase ◽  
Routine Procedure ◽  
Laboratory Conditions ◽  
Straight Line ◽  
Routine Clinical Laboratory

Abstract The procedure for serum alkaline phosphatase determination employing phenolphthalein monophosphate as substrate was evaluated under routine clinical laboratory conditions. A straight-line response to increasing serum concentration was observed, and the reaction was shown to be Mg++ dependent. The method proved to be extremely simple, and the results obtained correlated well with those given by a standard routine procedure involving determination of the liberated inorganic phosphate.

scholarly journals Evaluation of Serum Hepatocellular Enzymes in Nigerian with Goitre

2011 ◽  
Vol 2 (2) ◽  
pp. 79-81
Author(s):  
Johnkennedy Nnodim ◽  
Emejulu Adamma ◽  
Elendu Humphrey Ndubueze
Keyword(s):  
Cardiovascular Disease ◽  
Alkaline Phosphatase ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Normal Subjects ◽  
Medical Sciences ◽  
Free T4 ◽  
Imo State ◽  
And Control

Objective: The determination of serum gammaglutamyl transferase (GGT),alkaline phosphatase(ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in patient with goitre in Owerri, Imo state Nigeria were investigated.Material & Methods: Thirty confirmed patients with goitre age 50 to 70years with the following thyroid index (Total T4 > 140.65±7.28nmol/l , Total T3 > 2.43±0.96nmol/l, Free T4 < 50.24±9.11nmol/l and TSH > 4.12±1.00nmol/l) were selected for the study. Thirty normal subjects free from goiter age 50 to 70 years were used as control. Patients with complications such as cardiovascular disease, hypertension and diabetes were excluded.Results: The level of serum gamaglutamyl transferase in goitre subjects was significantly higher (50.32 ±4.27 iu/l) when compared with control (17.50±3.94 iu/l) at P< 0.05. In the same vein the level of alkaline phosphatase was significantly higher (110.9m/l±12.92 iu/l) when compared with the control (56.3±12.06 iu/l) P<0.05. The levels of AST and ALT in goitre and control were not significant when compared with the control.Conclusion: This observation shows that gammaglutamyl transferase and alkaline phosphatase are frequently in-creased in goitre. Hence, they are possibly thyroid dependent enzymes.Key Words: Gammaglutamyl transferase; alkaline phosphatase; aspartateaminotransferase; alanine aminotransferase; thyroid hormonesDOI: http://dx.doi.org/10.3126/ajms.v2i2.3870Asian Journal of Medical Sciences 2 (2011) 79-81

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