scholarly journals The Biological Behavior of Prostate Cancer Cells in 3D Culture Systems

2008 ◽  
Vol 128 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Masatoshi WATANABE ◽  
Akimitsu TAKAGI
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
3D Culture ◽  
Biological Behavior ◽  
Prostate Cancer Cells ◽  
Culture Systems

scholarly journals Development of EphA2 siRNA-loaded lipid nanoparticles and combination with a small‐molecule histone demethylase inhibitor in prostate cancer cells and tumor spheroids

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ezgi Oner ◽  
Mustafa Kotmakci ◽  
Anne-Marie Baird ◽  
Steven G. Gray ◽  
Bilge Debelec Butuner ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Transfection Efficiency ◽  
3D Culture ◽  
Lipid Nanoparticles ◽  
In Vivo Studies ◽  
Great Promise ◽  
Prostate Cancer Cells ◽  
Tumor Spheroids

Abstract Background siRNAs hold a great potential for cancer therapy, however, poor stability in body fluids and low cellular uptake limit their use in the clinic. To enhance the bioavailability of siRNAs in tumors, novel, safe, and effective carriers are needed. Results Here, we developed cationic solid lipid nanoparticles (cSLNs) to carry siRNAs targeting EphA2 receptor tyrosine kinase (siEphA2), which is overexpressed in many solid tumors including prostate cancer. Using DDAB cationic lipid instead of DOTMA reduced nanoparticle size and enhanced both cellular uptake and gene silencing in prostate cancer cells. DDAB-cSLN showed better cellular uptake efficiency with similar silencing compared to commercial transfection reagent (Dharmafect 2). After verifying the efficacy of siEphA2-loaded nanoparticles, we further evaluated a potential combination with a histone lysine demethylase inhibitor, JIB-04. Silencing EphA2 by siEphA2-loaded DDAB-cSLN did not affect the viability (2D or 3D culture), migration, nor clonogenicity of PC-3 cells alone. However, upon co-administration with JIB-04, there was a decrease in cellular responses. Furthermore, JIB-04 decreased EphA2 expression, and thus, silencing by siEphA2-loaded nanoparticles was further increased with co-treatment. Conclusions We have successfully developed a novel siRNA-loaded lipid nanoparticle for targeting EphA2. Moreover, preliminary results of the effects of JIB-04, alone and in combination with siEphA2, on prostate cancer cells and prostate cancer tumor spheroids were presented for the first time. Our delivery system provides high transfection efficiency and shows great promise for targeting other genes and cancer types in further in vitro and in vivo studies.

scholarly journals Repression of metadherin inhibits biological behavior of prostate cancer cells and enhances their sensitivity to cisplatin

2015 ◽  
Vol 12 (1) ◽  
pp. 226-232 ◽  
Author(s):  
YONG-BAO WEI ◽  
QIONG GUO ◽  
YUN-LIANG GAO ◽  
BIN YAN ◽  
ZHAO WANG ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Biological Behavior ◽  
Prostate Cancer Cells

Albumin–polymer conjugate nanoparticles and their interactions with prostate cancer cells in 2D and 3D culture: comparison between PMMA and PCL

2016 ◽  
Vol 4 (11) ◽  
pp. 2017-2027 ◽  
Author(s):  
Yanyan Jiang ◽  
Hongxu Lu ◽  
Aydan Dag ◽  
Gene Hart-Smith ◽  
Martina H. Stenzel
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
3D Culture ◽  
Prostate Cancer Cells ◽  
Polymer Conjugate ◽  
Self Assembled ◽  
Amphiphilic Nanoparticles ◽  
2D And 3D

Using proteins as the hydrophilic moiety can dramatically improve the biodegradability and biocompatibility of self-assembled amphiphilic nanoparticles in the field of nanomedicine.

279: Inhibition of Cyclooxygenase-2 Pathway Overcomes the Heat-Resistance in Prostate Cancer Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Makoto Sumitomo ◽  
Kenji Kuroda ◽  
Takako Asano ◽  
Akio Horiguchi ◽  
Keiichi Ito ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Heat Resistance ◽  
Cancer Cells ◽  
Cyclooxygenase 2 ◽  
Prostate Cancer Cells

574: Cellular Interaction Between Prostate Cancer and Bone Stroma: Effects of Radiation on the Growth and Colonization of Human Prostate Cancer Cells in Bone

2005 ◽  
Vol 173 (4S) ◽  
pp. 157-157
Author(s):  
Tohru Miyagi ◽  
Cynthia S. Anderson ◽  
Valerie A. Odero-Marah ◽  
Peter Johnstone ◽  
Leland W.K. Chung
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Human Prostate ◽  
Cellular Interaction ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Effects Of Radiation
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