Performance of 3 successive generations of specified-pathogen-free chickens maintained as a closed flock

1980 ◽  
Vol 14 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Kenji Furuta ◽  
Hitoshi Ohashi ◽  
Jitsuo Obana ◽  
Shizuo Sato
Keyword(s):  
Disease Virus ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Bronchitis ◽  
Avian Adenovirus ◽  
Bursal Disease ◽  
Salmonella Pullorum ◽  
Infectious Laryngotracheitis ◽  
Encephalomyelitis Virus ◽  
Laryngotracheitis Virus ◽  
Successive Generations

No antibodies against Salmonella pullorum, Mycoplasma gallisepticum, Mycoplasma synoviae, Haemophilus gallinarum, fowl pox virus, Marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and Newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. Antibodies against infectious bronchitis virus were detected in the sera of the 3rd generations at 66, 74 and 108 weeks of age. The performance of these chickens was nearly the same as that of conventional healthy chickens in the poultry industry, with no tendency to decline.

scholarly journals Use of degenerate oligonucleotide primed polymerase chain reaction for detection of chicken anaemia virus contamination in avian viral vaccines

2020 ◽  
Vol 23 (3) ◽  
pp. 304-309
Author(s):  
M. Jamshidian-Mojaver ◽  
S-E. Tabatabaeizadeh ◽  
M. Naeemipour ◽  
H. R. Farzin ◽  
M. R. Bassami
Keyword(s):  
Quality Control ◽  
Disease Virus ◽  
Specific Method ◽  
Infectious Laryngotracheitis Virus ◽  
Chicken Anaemia Virus ◽  
Degenerate Oligonucleotide ◽  
Bursal Disease ◽  
Infectious Laryngotracheitis ◽  
Viral Vaccines ◽  
Laryngotracheitis Virus

For quality control of biologicals of veterinary use, the absence of extraneous agents needs to be certified. One of the requirements for quality control of avian viral vaccines is to demonstrate freedom from extraneous and adventitious pathogenic agents, like chicken anaemia virus (CAV). In this study, a degenerate oligonucleotide primed PCR (DOP-PCR) for the detection of CAV was developed. Degenerate oligonucleotide primers were selected based on sequences corresponding to conserved regions of VP1 gene. After spiking of CAV genomic DNA to an infectious laryngotracheitis virus (ILTV) vaccine, detection limit for the test was 3.056×10-9 ng/µl. To evaluate the performance of the test, 11 avian viral vaccines including infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and ILTV vaccines from 5 manufacturers were screened for CAV and no contamination was detected. The test described here may provide a rapid, sensitive and specific method for contamination detection of avian viral vaccines with CAV, and may be applied for quality control of live and killed commercial vaccines.

scholarly journals A two-year prospective study of small poultry flocks in Ontario, Canada, part 1: prevalence of viral and bacterial pathogens

2019 ◽  
Vol 31 (3) ◽  
pp. 327-335 ◽  
Author(s):  
Nancy M. Brochu ◽  
Michele T. Guerin ◽  
Csaba Varga ◽  
Brandon N. Lillie ◽  
Marina L. Brash ◽  
...  
Keyword(s):  
Influenza A ◽  
Bacterial Pathogens ◽  
Animal Health ◽  
Salmonella Spp ◽  
Infectious Laryngotracheitis Virus ◽  
Zoonotic Pathogens ◽  
Increased Risk ◽  
Bursal Disease ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus

In Ontario, within the past few years, there has been a marked increase in the number of non-commercial poultry flocks (referred to as “small flocks”). Small poultry flocks may act as a reservoir of avian and zoonotic pathogens, given the flocks’ limited access to veterinary services, inadequate biosecurity practices, and increased risk of contact with wild birds. Despite these potential risks, there is a scarcity of data concerning the prevalence of poultry and zoonotic pathogens among these flocks. To assess the baseline prevalence of bacterial and viral infectious pathogens, prospective surveillance of small flock postmortem submissions to the Animal Health Laboratory was conducted over a 2-y period. With the owner’s consent, a postmortem examination and pre-set tests for infectious agents were conducted. A total of 160 submissions, mainly chickens (84%), were received. Among bacterial pathogens, Brachyspira spp., Mycoplasma synoviae, Campylobacter spp., Mycoplasma gallisepticum, and Salmonella spp. were detected in 37%, 36%, 35%, 23%, and 3% of tested submissions, respectively. Among viral pathogens, infectious bronchitis virus, fowl adenovirus, infectious laryngotracheitis virus, avian reovirus, and infectious bursal disease virus were detected in 39%, 35%, 15%, 4%, and 1% of submissions, respectively. We detected non-virulent avian avulavirus 1 from two chickens in a single submission, and low-pathogenic H10N8 influenza A virus from a single turkey submission. Our study provides baseline prevalence of viral and bacterial pathogens circulating in Ontario small flocks and may help animal and human health professionals to educate small flock owners about disease prevention.

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