scholarly journals Effect of the Polyvinylpyrrolidone Concentration of Cryoprotectant on Mouse Embryo Development and Production of Pups: 7.5% of PVP is Beneficial for In Vitro and In Vivo Development of Frozen-Thawed Mouse Embryos

2008 ◽  
Vol 54 (4) ◽  
pp. 250-253 ◽  
Author(s):  
Chi-Gu KIM ◽  
Hwanyul YONG ◽  
Gene LEE ◽  
Jaejin CHO
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryos ◽  
Mouse Embryo Development

Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

Zygote ◽  
1995 ◽  
Vol 3 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Elena Ibánez ◽  
Francesca Vidal ◽  
Juan Hidalgo
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Normal Mouse ◽  
Polyclonal Antibodies ◽  
Experimental Treatment ◽  
Mouse Embryos ◽  
Mouse Embryo Development ◽  
Morula Stage ◽  
Early Mouse

SummaryPolyclonal antibodies that cross-react with rodent metallothionein I (MT I) and metallothionein II (MT II) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes.

In vivo cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells

1989 ◽  
Vol 1 (2) ◽  
pp. 127 ◽  
Author(s):  
D Sakkas ◽  
AO Trounson ◽  
I Kola
Keyword(s):  
Embryo Development ◽  
Calf Serum ◽  
Mouse Embryos ◽  
Early Cleavage ◽  
Cleavage Rate ◽  
Cell Stage ◽  
Mouse Embryo Development ◽  
Embryo Culture Medium

The cleavage rate and development of two-cell mouse embryos to the morulae stage in co-culture with mouse oviduct cells was studied in vitro and compared with those achieved in vivo. Embryos were cultured in Whittingham's T6 (T6), T6 supplemented with fetal calf serum (FCS) and in co-culture with either Dulbecco's Modified Eagles Medium supplemented with sodium lactate (DMEM + 1a) or a modification of T6 medium containing vitamins and amino acids (T6 + v + aa). Co-culture of oviductal cells with DMEM + la medium supported two-cell mouse embryo development to eight cells at a rate significantly better (P less than 0.001) than T6, but the rate of embryo development was not equivalent to that in vivo. DMEM + la alone was inadequate as an embryo culture medium. Co-cultures using T6 + v + aa with mouse oviductal cells were prepared from mice at days 1, 2 or 3 of pseudopregnancy. Day 2 and 3 co-cultures allowed two-cell embryos to develop at a rate comparable to that in vivo up to the mid eight-cell stage (68 h after hCG), but by 76 h after hCG embryos were retarded. Transfer to pseudopregnant recipients of embryos co-cultured with day 2 oviductal cells until 68 h after hCG resulted in a rate of fetal development equivalent to that of embryos grown in vivo. Our results show that co-culture of early cleavage-stage embryos with mouse oviductal cells allows embryos to retain cleavage rates and viability comparable to in vivo development.

Comparative effects of essential and nonessential metals on preimplantation mouse embryo development in vitro

Toxicology ◽  
1997 ◽  
Vol 116 (1-3) ◽  
pp. 123-131 ◽  
Author(s):  
Lynn A. Hanna ◽  
Jeffrey M. Peters ◽  
Lynn M. Wiley ◽  
Michael S. Clegg ◽  
Carl L. Keen
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

scholarly journals Effects of peritoneal fluid on preimplantation mouse embryo development and apoptosis in vitro

2005 ◽  
Vol 11 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Navid Esfandiari ◽  
Tommaso Falcone ◽  
Jeffrey M Goldberg ◽  
Ashok Agarwal ◽  
Rakesh K Sharma
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Peritoneal Fluid ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

In vitro effect of levonorgestrel on sperm fertilizing capacity and mouse embryo development

Contraception ◽  
2005 ◽  
Vol 72 (1) ◽  
pp. 71-76 ◽  
Author(s):  
María José Munuce ◽  
Josiane A.A. Nascimento ◽  
Germán Rosano ◽  
Anibal Faúndes ◽  
Karen Saboya-Brito ◽  
...  
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Vitro Effect
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