Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis

2007 ◽  
Vol 103 (6) ◽  
pp. 563-567 ◽  
Author(s):  
Ping Wang ◽  
Xiuting Li ◽  
Mufei Xiang ◽  
Qian Zhai
Keyword(s):  
16S Rrna ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
16S Rrna Analysis

Characterization of oral bacteria in the tongue coating of patients with halitosis using 16S rRNA analysis

2020 ◽  
Vol 78 (7) ◽  
pp. 541-546 ◽  
Author(s):  
Akiko Oshiro ◽  
Takashi Zaitsu ◽  
Masayuki Ueno ◽  
Yoko Kawaguchi
Keyword(s):  
16S Rrna ◽  
Oral Bacteria ◽  
Tongue Coating ◽  
16S Rrna Analysis

scholarly journals Seasonal Dynamics of the Activated Sludge Microbiome in Sequencing Batch Reactors, Assessed Using 16S rRNA Transcript Amplicon Sequencing

2020 ◽  
Vol 86 (19) ◽  
Author(s):  
Juliet Johnston ◽  
Sebastian Behrens
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
16S Rrna ◽  
Activated Sludge ◽  
Seasonal Dynamics ◽  
Activity Patterns ◽  
Amplicon Sequencing ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Copy Numbers

ABSTRACT Activated sludge is comprised of diverse microorganisms which remediate wastewater. Previous research has characterized activated sludge using 16S rRNA gene amplicon sequencing, which can help to address questions on the relative abundance of microorganisms. In this study, we used 16S rRNA transcript sequencing in order to characterize “active” populations (via protein synthesis potential) and gain a deeper understanding of microbial activity patterns within activated sludge. Seasonal abundances of individual populations in activated sludge change over time, yet a persistent group of core microorganisms remains throughout the year which are traditionally classified on presence or absence without monitoring of their activity or growth. The goal of this study was to further our understanding of how the activated sludge microbiome changes between seasons with respect to population abundance, activity, and growth. Triplicate sequencing batch reactors were sampled at 10-min intervals throughout reaction cycles during all four seasons. We quantified the gene and transcript copy numbers of 16S rRNA amplicons using real-time PCR and sequenced the products to reveal community abundance and activity changes. We identified 108 operational taxonomic units (OTUs) with stable abundance, activity, and growth throughout the year. Nonproliferating OTUs were commonly human health related, while OTUs that showed seasonal abundance changes have previously been identified as being associated with floc formation and bulking. We observed significant differences in 16S rRNA transcript copy numbers, particularly at lower temperatures in winter and spring. The study provides an analysis of the seasonal dynamics of microbial activity variations in activated sludge based on quantifying and sequencing 16S rRNA transcripts. IMPORTANCE Sequencing batch reactors are a common design for wastewater treatment plants, particularly in smaller municipalities, due to their low footprint and ease of operations. However, like for most treatment plants in temperate/continental climates, the microbial community involved in water treatment is highly seasonal and its biological processes can be sensitive to cold temperatures. The seasonality of these microbial communities has been explored primarily in conventional treatment plants and not in sequencing batch reactors. Furthermore, most studies often only address which organisms are present. However, the activated sludge microbial community is very diverse, and it is often hard to discern which organisms are active and which organisms are simply present. In this study, we applied additional sequencing techniques to also address the issues of which organisms are active and which organisms are growing. By addressing these issues, we gained new insights into seasonal microbial populations dynamics and activity patterns affecting wastewater treatment.

Taxonomic Studies of Predatory Bdellovibrios Based on 16S rRNA Analysis, Ribotyping and the hit Locus and Characterization of Isolates from the Gut of Animals

2001 ◽  
Vol 24 (3) ◽  
pp. 385-394 ◽  
Author(s):  
Dominik Schwudke ◽  
Eckhard Strauch ◽  
Monika Krueger ◽  
Bernd Appel
Keyword(s):  
16S Rrna ◽  
16S Rrna Analysis ◽  
Taxonomic Studies

scholarly journals Functional Metagenomics Characterization of an Anaerobic Saltwater Bioreactor

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
D. Derilus ◽  
A. Forestil ◽  
J. Fortuné ◽  
O. Polyanska ◽  
C. Louime ◽  
...  
Keyword(s):  
Renewable Energy ◽  
16S Rrna ◽  
Microbial Communities ◽  
Common Property ◽  
Marine Macroalgae ◽  
Functional Metagenomics ◽  
16S Rrna Analysis ◽  
Potential Applications ◽  
Tree Building

Methanogens are restricted to a few genera of Archaea, however they have great importance in the carbon cycle, impacting climactic considerations, and also find a role in renewable energy in the form of biogas. Here, we examine the microbial contribution to the production of methane in a sargassum fed anaerobic saltwater bioreactor, which are poorly characterized compared to fresh water bioreactors, using a comprehensive functional metagenomics approach. Despite abundant production of methane, we detected a low proportion of Archaea in the system using 16S rRNA community profile analyses. We address the low representation using an additional 16S rRNA analysis of shotgun data and a consideration of CO2:CH4production. Using a novel network alignment and tree building approach, we measured similarity between the meta-metabolic capabilities of different anaerobic microbial communities. The saltwater bioreactor samples clustered together, validating the approach and providing a method of determining meta-metabolic similarity between microbial communities, with a range of potential applications. We also introduce a number of additional approaches for examining and interpreting meta-metabolic network topology. The low abundance of methanogens appears as a common property of such anaerobic systems and likely reflects the relatively poor energetics of methanogens, while examination of key enzymes confirms that hydrogen producing bacteria are the major fermentative guild. Our results indicate that the use of readily available seawater and marine macroalgae is a promising approach to the production of biogas as a source of renewable energy.

scholarly journals Molecular characterization of nocardioform actinomycetes in activated sludge by 16S rRNA analysis

Microbiology ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 513-521 ◽  
Author(s):  
M. Schuppler ◽  
F. Mertens ◽  
G. Schon ◽  
U. B. Gobel
Keyword(s):  
16S Rrna ◽  
Activated Sludge ◽  
Molecular Characterization ◽  
16S Rrna Analysis

Model-based characterization of endogenous maintenance, cell death and predation processes of activated sludge in sequencing batch reactors

2011 ◽  
Vol 66 (4) ◽  
pp. 747-754 ◽  
Author(s):  
Bing-Jie Ni ◽  
Guo-Ping Sheng ◽  
Han-Qing Yu
Keyword(s):  
Cell Death ◽  
Activated Sludge ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Model Based

COMPARISON OF DIFFERENT DURATION OF ANAEROBIC AND AEROBIC PHASES ON ACID RED 18 REMOVAL IN SEQUENCING BATCH REACTORS

2016 ◽  
Vol 15 (11) ◽  
pp. 2529-2535
Author(s):  
Mohammad Reza Alavi Moghaddam ◽  
Mohammad Hakimelahi ◽  
Seyed Hossein Hashemi
Keyword(s):  
Batch Reactors ◽  
Sequencing Batch Reactors

IMPLEMENTATION AND EVALUATION OF ENERGY SAVINGS FOCUSED AERATION DESIGN AND CONTROL OF SEQUENCING BATCH REACTORS IN WASHINGTON

2016 ◽  
Vol 2016 (9) ◽  
pp. 743-749
Author(s):  
Gregory J Duffy ◽  
Peter Burgoon ◽  
Steven Kestel
Keyword(s):  
Energy Savings ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
And Control

Molecular characterization of the bacterial communities present in sheep's milk and cheese produced in South Brazilian Region via 16S rRNA gene metabarcoding sequencing

LWT ◽  
2021 ◽  
Vol 147 ◽  
pp. 111579
Author(s):  
Creciana M. Endres ◽  
Ícaro Maia S. Castro ◽  
Laura D. Trevisol ◽  
Juliana M. Severo ◽  
Michele B. Mann ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Bacterial Communities ◽  
Rrna Gene

scholarly journals Effect of magnetic field on biomass properties and their role in biodegradation under condition of low dissolved oxygen

2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Nur Syamimi Zaidi ◽  
Johan Sohaili ◽  
Khalida Muda ◽  
Mika Sillanpää ◽  
Norelyza Hussein
Keyword(s):  
Magnetic Field ◽  
Dissolved Oxygen ◽  
Biomass Concentration ◽  
Aerobic Bacteria ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Great Chance ◽  
Sludge Bulking ◽  
The Magnetic Field ◽  
High Biomass Concentration

AbstractLow condition of dissolved oxygen (DO) is commonly associated with sludge bulking problem that was able to disrupt the efficiency of wastewater treatment performances. Relatively, very little attention was paid to the possibility of applying magnetic field in controlling the bulking problem. Hence, this study aims to investigate the performance of magnetic field on biomass properties and its effect on biodegradation under low condition of DO. Two continuous laboratory-scale sequencing batch reactors—Reactor A (SBRA) and Reactor B (SBRB)—were setup. SBRA was equipped with the magnetic device to exhibit magnetic field of 88 mT, while SBRB acted as a control system. The results showed that the biomass concentration in SBRA was higher compared to SBRB. High biomass concentration in SBRA resulted to better settleability with mean SVI of less than 30 mL/g. SBRA also showed consistently high removal performances of organic and inorganic contents compared to SBRB. These observations confirmed that the magnetic field was able to enhance the biomass properties, which further enhance the biodegradation ability of the aerobic bacteria under low DO condition. This also indicates that under the sludge bulking circumstances, the use of magnetic field stands a great chance in maintaining high biodegradation of the treatment system.

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