Genetic control of fission yeast cell wall synthesis: the genes involved in wall biogenesis and their interactions in Schizosaccharomyces pombe.

1. A study of wall synthesis has been made by following the incorporation of radioactive glucose and threonine into the cytoplasm and wall of yeast. 2. Both glucose and threonine are incorporated into a mannan glycopeptide. The glucose is also synthesized into a structural glucan of the wall. 3. The mannan glycopeptide contains high-molecular-weight mannan and low-molecular-weight mannose and oligosaccharide units composed of mannose. Both types of carbohydrate are attached to the peptide. The extent of radioactive incorporation into these different carbohydrate constituents of the glycopeptide remained constant during a pulse-chase experiment. No evidence of a sequential synthesis of oligosaccharides and high-molecular-weight mannan was obtained. 4. Cycloheximide inhibits the incorporation of threonine into the wall but only partially inhibits the incorporation of glucose. Thus not all the polysaccharide deposited into the wall is dependent on a simultaneous peptide synthesis and incorporation. 5. Protoplasts grown in an iso-osmotic medium secreted a mannan polymer that was probably a glycopeptide.

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Effect of cycloheximide on yeast cell wall synthesis

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(69)90672-x ◽

1969 ◽

Vol 36 (5) ◽

pp. 741-747 ◽

Cited By ~ 28

Author(s):

M.V. Elorza ◽

R. Sentandreu

Keyword(s):

Cell Wall ◽

Yeast Cell ◽

Yeast Cell Wall ◽

Cell Wall Synthesis

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Domains determining the functional distinction of the fission yeast cell cycle "start" molecules Res1 and Res2.

Molecular Biology of the Cell ◽

10.1091/mbc.7.12.1967 ◽

1996 ◽

Vol 7 (12) ◽

pp. 1967-1976 ◽

Cited By ~ 8

Author(s):

S Sturm ◽

H Okayama

Keyword(s):

Cell Cycle ◽

Yeast Cell ◽

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Yeast Cell Cycle ◽

Functional Specificity ◽

Conserved Motifs ◽

Fission Yeast Cell ◽

Discrete Domains ◽

Meiotic Cycle

In Schizosaccharomyces pombe the "start" of the cell cycle is regulated by two parallel, functionally overlapping complexes composed of Res1-Cdc10 and Res2-Cdc10. Res1 and Res2 are structurally very homologous and are required for the start of the mitotic and meiotic cycle, respectively. We have addressed the question which parts of the proteins are essential for function and determine the functional specificity. Several discrete domains in the nonconserved C-terminal region are essential for the mitotic and meiotic start function and determine the functional specificity independently of the structurally conserved motifs at the N-terminal end and in the center. One of these domains in Res2 restricts Res2 to interact only with Rep2. Res2 without this domain behaves like a functional chimera having the properties of Res2 and Res1. Likewise, internally truncated forms of Res1 lacking the centrally located ankyrin repeats and adjacent sequences can partially suppress the meiotic defect in res2- cells. These truncated Res1 molecules behave like functional chimeras with the properties of Res1 and Res2.

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