scholarly journals A DIFFERENTIAL STAINING METHOD FOR A- AND B-CELLS IN THE PANCREATIC ISLETS OF LANGERHANS

1977 ◽  
Vol 10 (1) ◽  
pp. 10-13 ◽  
Author(s):  
YASUMITSU KIKUI ◽  
HARUMICHI SEGUCHI ◽  
HUMIO MIZOGUTI
Keyword(s):  
B Cells ◽  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Staining Method ◽  
Differential Staining ◽  
Pancreatic Islets Of Langerhans ◽  
A And B Cells

scholarly journals CYTOCHEMICAL LOCALIZATION OF ADENYL CYCLASE ACTIVITY IN RAT ISLETS OF LANGERHANS

1972 ◽  
Vol 20 (11) ◽  
pp. 873-879 ◽  
Author(s):  
S. L. HOWELL ◽  
MARGARET WHITFIELD
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Adenosine Triphosphate ◽  
Adenyl Cyclase ◽  
Cyclase Activity ◽  
Cytochemical Localization ◽  
Adenyl Cyclase Activity ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
A And B Cells

A cytochemical method has been used to investigate the localization of adenyl cyclase activity in A and B cells of isolated rat islets of Langerhans. Adenosine triphosphate was initially utilized as substrate, the pyrophosphate liberated being precipitated by lead ions at its site of production. The specificity of the method was increased by the use of adenylyl-imidodiphosphate as an alternative substrate; this adenosine triphosphate analogue was not hydrolyzed by adenosine triphosphatase but provided an effective substrate for adenyl cyclase. Adenyl cyclase activity, which was found to retain its glucagon and fluoride sensitivity in glutaraldehyde-fixed tissue, was found exclusively and almost uniformly in the plasma membranes of A and B cells. Storage granule membrane, incorporated into the plasma membrane during secretion of the granule content by exocytosis, appeared to be devoid of adenyl cyclase activity.

Altered TNF-α, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system

2001 ◽  
Vol 280 (1) ◽  
pp. E92-E102 ◽  
Author(s):  
Brian W. Tobin ◽  
Sandra K. Leeper-Woodford ◽  
Brian B. Hashemi ◽  
Scott M. Smith ◽  
Clarence F. Sams
Keyword(s):  
Cell Culture ◽  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Three Dimensional ◽  
Model System ◽  
Culture Methods ◽  
Static Culture ◽  
Pancreatic Islets Of Langerhans ◽  
Tnf Α ◽  
Factor Α

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 ± 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-α (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group ( P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture ( P< 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system ( P < 0.05). These studies demonstrate alterations in LPS-induced TNF-α production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.

A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans

Metabolism ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Jack Noel ◽  
Alexander Rabinovitch ◽  
Les Olson ◽  
George Kyriakides ◽  
Joshua Miller ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Large Scale ◽  
High Yield ◽  
Pancreatic Islets Of Langerhans

SURVIVAL OF CRYOPRESERVED ISOLATED ADULT HUMAN PANCREATIC ISLETS OF LANGERHANS

1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
GARTH L. WARNOCK ◽  
DEREK W. R. GRAY ◽  
PHILIP MCSHANE ◽  
MAUREEN PETERS ◽  
PETER J. MORRIS
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Human Pancreatic Islets ◽  
Adult Human ◽  
Pancreatic Islets Of Langerhans
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