scholarly journals DNA barcoding of twelve shrimp species (Crustacea: Decapoda) from Turkish seas reveals cryptic diversity

2014 ◽  
Vol 16 (1) ◽  
pp. 36 ◽  
Author(s):  
R. BILGIN ◽  
M. A. UTKAN ◽  
E. KALKAN ◽  
S. U. KARHAN ◽  
M. BEKBÖLET

DNA barcoding is a useful tool for the identification and potential discovery of new species. In this study, DNA barcoding was employed by sequencing the mitochondrial cytochrome oxidase subunit I gene (COI) to characterize the genetic diversity of 12 shrimp species inhabiting Turkish coastal waters and, when possible, to compare with the genetic data available from different parts of the Mediterranean and eastern Atlantic. This study also comprises the first DNA barcoding study performed in the Turkish Seas using COI. A total of 40 shrimp specimens were collected and analyzed from 9 sites. Generally, the barcoding gap criterion was successful at identifying species; hence COI appeared to be a good marker of choice for DNA barcoding in this group. Out of the 12 species investigated, five were barcoded for the first time. In six species two intraspecific clades were retrieved after the analyses. The results suggest the presence of cryptic diversity in a genetically understudied marine area, Turkish coastal waters, and further investigation in these species using population genetics, taxonomic approaches and nuclear markers is likely to result in designation of new species.


Zootaxa ◽  
2020 ◽  
Vol 4778 (2) ◽  
pp. 396-400
Author(s):  
HASSAN NAVEED ◽  
XIU-DAN WANG ◽  
CHENGQUAN CAO ◽  
YALIN ZHANG

The genus Olidiana McKamey, 2006 is reported for the first time from Pakistan based on the new species Olidiana pakistanica sp. nov. Photographic illustrations and a description are provided for the habitus and male genitalia of this new species. A partial mitochondrial cytochrome oxidase I gene (COI) sequence is also provided. 



Crustaceana ◽  
2021 ◽  
Vol 94 (10) ◽  
pp. 1201-1220
Author(s):  
Qinghua Chen ◽  
Wenjian Chen ◽  
Yuanwei Hu ◽  
Ka Yan Ma ◽  
Zhaoliang Guo

Abstract This study dealt with three species of ornamental palaemonid freshwater prawns of the genus Macrobrachium, based on morphological and molecular analysis. Macrobrachium pentazona He, Gao & Guo, 2009; M. laevis Zheng, Chen & Guo, 2019; and M. bilineare sp. nov. are distinguishable from closely related species by segmental ratios, spination of the second pereiopods and the slender scaphocerite. Macrobrachium bilineare sp. nov. can easily be recognized in the field by its bright colour pattern. Molecular evidence of mitochondrial cytochrome oxidase subunit I (COI), also supports the characterization of this new species, raising the total number of Macrobrachium spp. known from China to 41. Detailed description, illustrations, colour photographs, habitat information, distribution maps and features of conservation significance are also briefly discussed.



Zootaxa ◽  
2019 ◽  
Vol 4585 (3) ◽  
pp. 547
Author(s):  
VALENTINA A. TESLENKO ◽  
DMITRY M. PALATOV ◽  
ALEXANDER A. SEMENCHENKO

Leuctra adjariae sp. n. and Leuctra georgiae sp. n. (Plecoptera: Leuctridae) are described as two new apterous stonefly species from the Meskheti Range (Lesser Caucasus) in southwestern Georgia. Descriptions and illustrations are provided for both sexes and diagnostic characters are discussed. Males and females of the two species are associated by DNA barcodes. Comparisons with corresponding regions of COI between L. adjariae sp. n. and L. georgiae sp. n. produced K2P genetic distances of 8.38%, values well associated with interspecific variation. The well-supported monophyly as well as results of an ABGD analysis confirms the validity of both new species. Capnioneura gouanerae Vinçon & Sivec, 2011, previously described and known only from Turkey, is reported for the first time for the Caucasus. 



Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 



Zootaxa ◽  
2019 ◽  
Vol 4560 (2) ◽  
pp. 375
Author(s):  
SHI-JING CHEN ◽  
ZUO-GANG PENG

A new species of Nemacheilidae, Triplophysa sanduensis sp. nov., is described here based on specimens collected from a small cave in Dengguang Village, Zhonghe Town, Sandu County, Guizhou Province, China. The new species can be distinguished from its congeners by the following combination of characters: branched dorsal-fin rays 8–9; branched pectoral-fin rays 8–9; branched pelvic-fin rays 5; branched anal-fin rays 5; vertebrae 4+37; snout length 45.5–50.1% of head length; postdorsal length 39.0–42.8% of standard length; caudal-peduncle depth/caudal-peduncle length range from 56.4% to 71.9%; the posterior chamber of the gas bladder degenerated; body elongated, covered by scattered scales; eyes present and normal in size, not reduced or absent; and a complete lateral line. A phylogenetic analysis based on the mitochondrial cytochrome oxidase subunit 1 (COI) gene resolved Triplophysa sanduensis as sister to Triplophysa longliensis. Kimura's 2-parameter genetic distance of the COI gene sequences between these two species was 1.9%. 



Author(s):  
Catalina T. Pastor de Ward ◽  
Virginia Lo Russo

This paper focuses on Diplolaimella and Diplolaimelloides two very cosmopolitan genera inhabiting lotic freshwater to salty water inland, groundwater and coastal waters systems, providing a review of their taxonomy with new keys, and their distribution on the east coast of Patagonia and inland Patagonia. Two new species belonging to the genus Diplolaimelloides of the family Monhysteridae are described from Patagonian salty lagoons and coastal areas, and the Chubut and Santa Cruz provinces of Argentina: Diplolaimelloides tehuelchus sp. nov. has four bursal and three postcloacal papillae, short gubernaculum, and spicules longer than 50 μm; it has been found in Patagonian salty lagoons and in high coastal salt marsh areas. Diplolaimelloides warwicki sp. nov. is characterized by the angular shape of the spicules and the long gubernaculum. Diplolaimella ocellata is recorded for the first time in Patagonian salty lagoons and new localities in coastal waters are recorded for Diplolaimella gerlachi Pastor de Ward, 1984.



Zootaxa ◽  
2012 ◽  
Vol 3273 (1) ◽  
pp. 28 ◽  
Author(s):  
MICHEL P. VALIM ◽  
RICARDO L. PALMA

Redescriptions of Bizarrifrons magus (Nitzsch [in Giebel], 1866), the type species of Bizarrifrons, and B. picturatus Car-riker & Díaz-Ungría, 1961 are given based on material from their type hosts. The nymphal instars of these two species aredescribed and illustrated for the first time. Also, three new species are named and described: B. latifrons, from the russet-backed oropendola, Psarocolius angustifrons alfredi (Des Murs, 1856); B. wecksteini, from the Amazonian oropendola,Psarocolius b. bifasciatus (Spix, 1824); and B. quasisymmetricus, from the solitary cacique, Cacicus solitarius (Vieillot,1816) (Passeriformes: Icteridae). Two species-groups are proposed, and a checklist and a key for the species of Bizarri-frons are also included. Sequences of a portion of the mitochondrial cytochrome oxidase I (COI) and the nuclear elongation factor 1α (EF-1α) genes for two species are given for the first time in this genus.





2019 ◽  
Vol 11 (1) ◽  
pp. 111-120 ◽  
Author(s):  
M. F. Akinwunmi ◽  
A. O. Lawal-Are

Species identification by morphological approach requires a high degree of experience, which is difficult and not practical enough for those interested in surveying a broad diversity of organisms. However, molecular or genetic approaches to identify species have been proposed and widely used by most scientists. DNA barcoding was used to identify Macrobrachium species occurring in three coastal lagoons (Badagry, Lagos and Epe) in South-western Nigeria. Samples of prawns for the DNA studies were collected from May to July 2015. The DNA of the Macrobrachium species was extracted using the phenol-chloroform protocol and Norgen tissue kit. Amplification and sequencing of the Macrobrachium species were carried out and further identification was done by comparing with sequenced data in the Genbank. New species of Macrobrachium were obtained in Badagry and Epe Lagoons having a close match with M. asperulum and M. nipponense at 85% and 84% respectively. M. asperulum and M. nipponense are reported in Badagry and Epe Lagoons for the first time.



Zootaxa ◽  
2017 ◽  
Vol 4227 (4) ◽  
pp. 563 ◽  
Author(s):  
BEHNAM MOTAMEDINIA ◽  
CHRISTIAN KEHLMAIER ◽  
AZIZOLLAH MOKHTARI ◽  
EHSAN RAKHSHANI ◽  
EBRAHIM GILASIAN

The genus Claraeola Aczél is recorded from Iran for the first time. Two new species, Claraeola parnianae Motamedinia & Kehlmaier sp. nov. and Claraeola khorshidae Motamedinia & Kehlmaier sp. nov., are described and illustrated. An updated identification key to the Western Palaearctic species of the genus Claraeola is provided. Both species were characterized morphologically and by DNA barcoding of the mitochondrial COI gene. 



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